Chemical Compositions and Antioxidant Activity of Volatile Oils from Doellingeria scaber

Bin ZHANG; Lei ZHANG; Fen TAN; Hong-quan ZHANG; Tian WU

doi:10.13422/j.cnki.syfjx.20190316

您当前的位置：

首页 >

文章列表页 >

Chemical Compositions and Antioxidant Activity of Volatile Oils from Doellingeria scaber

Resource and Quality Evaluation | 更新时间：2021-02-09

- Chemical Compositions and Antioxidant Activity of Volatile Oils from Doellingeria scaber
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 4, Pages: 187-192(2019)
- 作者机构：
  
  湖北第二师范学院　化学与生命科学学院，植物抗癌活性物质提纯与应用湖北省重点实验室，武汉　 430205
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190316
  CLC： R284.1;R289;R22
- Received：30 March 2018，
  
  Published Online：18 November 2018，
  
  Published：20 February 2019
- 稿件说明：
移动端阅览
Bin ZHANG, Lei ZHANG, Fen TAN, et al. Chemical Compositions and Antioxidant Activity of Volatile Oils from Doellingeria scaber[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 187-192.
DOI：

Bin ZHANG, Lei ZHANG, Fen TAN, et al. Chemical Compositions and Antioxidant Activity of Volatile Oils from Doellingeria scaber[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 187-192. DOI： 10.13422/j.cnki.syfjx.20190316.

摘要

目的：

对产于大别山高海拔地区的野生东风菜挥发油进行化学成分鉴定及相对含量分析，并研究其抗氧化能力。

方法：

采用同时蒸馏萃取法(SDE)提取东风菜全草挥发油，通过气相色谱-质谱联用(GC-MS)技术鉴定其中化学成分，采用峰面积归一化法确定各组分的相对百分含量。采用铁离子还原法，1，1-二苯基-2-三硝基苯肼(DPPH)法，

-胡萝卜素漂白法测定东风菜挥发油体外抗氧化活性，

结果：

东风菜挥发油共鉴定出50种化学成分，占总相对含量的86.91%，主要化学成分为反式-

-金合欢烯(20.21%)，吉马烯D(9.94%)，棕榈酸(8.66%)，

-萜品烯(7.82%)，石竹烯(6.9%)和对伞花烃-8-醇(4.48%)，其中烯类56.57%，醇类12.24%，脂肪酸类11.24%，环氧化物2.93%，酯类1.82%，醛酮类1.60%和芳香烃类0.51%。挥发油对铁离子的还原能力随浓度增加而增大，但还原能力远弱于维生素C，对DPPH自由基和在

-胡萝卜素/亚油酸脂质体系中的半抑制浓度(IC

)分别为0.72 g·L

－1

和0.10 g·L

－1

，表明挥发油对DPPH自由基有一定清除能力，且具有较好的脂质过氧化抑制能力。

结论：

通过GC-MS鉴别了东风菜挥发油化学成分，体外抗氧化实验表明其具有一定的抗氧化能力，为东风菜的开发利用提供实验依据。

Abstract

Objective:

To identify the chemical compositions and conduct relative content analysis of the essential oil from wild

Doellingeria scaber

in high altitude area of Dabie mountains

and investigate its antioxidant capacity.

Method:

The volatile oil of

scaber

was extracted by simultaneous distillation extraction (SDE) method. Its chemical compositions were analyzed by gas chromatography-mass spectometry (GC-MS)

and the relative percentage content of each composition was determined by peak area normalization method. The antioxidant activities of the oil were evaluated by reducing ability method

1-diphenyl-2-picrylhydrazyl (DPPH) method and

-carotene bleaching assay.

Result:

A total of 50 components representing 86.91% in this plant were identified. The main chemical compositions were (

-farnesene(20.21%)

germacrene D(9.94%)

hexadecanoic acid(8.66%)

-terpinene(7.82%)

caryophyllene(6.9%) and

-cymen-8-ol(4.48%). The main components of volatile oils include alkenes (56.57%)

alcohols (12.24%)

fatty acids (11.24%)

epoxides(2.93%)

esters(1.82%)

aldehydes and ketones (1.60%)

and aromatics (0.51%). The reduction ability of volatile oil to iron ions increases with the increase of concentration

but the reduction ability is far weaker than that of vitamin C. The 50% inhibiting concentration (IC

) of volatile oil on DPPH free radicals and in

-carotene/linoleic acid lipid system was 0.72 g·L

-1

and 0.10 g·L

-1

respectively

indicating that the volatile oil had a certain scavenging capacity on DPPH free radicals and a good inhibition effect on lipid peroxidation.

Conclusion:

GC-MS was used to identify the chemical compositions of the volatile oil from

scaber

and antioxidant test in vitro showed that

scaber

had certain antioxidant capacity

providing experimental basis for its development and utilization.

关键词

Keywords

references

中国科学院《中国植物志》编辑委员会．中国植物志 [M]. 北京：科学出版社， 1985 ： 128 .

南京中医药大学．中药大词典 [M]. 2版．上海：上海科学技术出版社， 2006 ： 891 - 892 .

刘淑兰．东风菜的药用研究概况 [J]. 中医药信息， 2007 , 24 ( 3 ): 18 - 20 .

刘祝安，杜一新．东风菜资源的保护和开发利用思考 [J]. 中国园艺文摘， 2014 , 30 ( 5 ): 66,208 .

白素平，范秉琳，闫福林．东风菜中甾体成分研究 [J]. 新乡医学院学报， 2005 , 22 ( 3 ): 185 - 187 .

匡海学，张鹏，杨炳友，等．东风菜根生物活性成分的研究 [J]. 中医药学报， 1999 ( 2 ): 54 - 55 .

Kwon H C , Jung C M , Shin C G , et al . A new caffeoyl quinic acid from Aster Scaber and its inhibitory activity against human immunodeficiency virus-1(HIV-1) integrase [J]. Chem Pharm Bull , 2000 , 48 ( 11 ): 1796 - 1798 .

蒋金和，邓雪琳，王利勤，等．东风菜化学成分及药理活性研究进展 [J]. 中成药， 2008 , 30 ( 10 ): 1517 - 1520 .

Cho Y O . Antioxidative activity of the korean wild leafy vegetables：Aster Scaber and Ligularia Fischeri [J]. J Food Sci Nutr , 2002 , 7 ( 2 ): 146 - 150 .

Sok D E , Oh S H , Kim A T , et al . Neuroprotective effect of rough aster butanol fraction against oxidative stress in the brain of mice challenged with kainic acid [J]. J Agric Food Chem , 2004 , 51 ( 16 ): 4570 - 4575 .

Mcreynolds W O . Gas Chromatographic Retention Data [M]. New York : Technical Abstracts Co,Evanston Ⅲ , 1966 .

张婷婷，罗丽萍，黄学勇，等．辛夷挥发油GC-MS分析及其抗氧化、抗菌活性 [J]. 食品科学， 2016 , 37 ( 10 ): 144 - 150 .

WANG D C , SUN S H , SHI L N , et al . Chemical composition，antibacterial and antioxidant activity of the essential oils of Metaplexis Japonica and their antibacterial components [J]. Int J Food Sci Technol , 2015 , 50 ( 2 ): 449 - 457 .

Taga M S , Miller E E , Pratt D E. Chia seeds as a source of natural lipids antioxidants [J]. J Am Oil Chem Soc , 1984 , 61 ( 5 ): 928 - 931 .

逄楠楠，于勇，毕开顺，等． GC法同时测定芫花中棕榈酸与亚油酸的含量 [J]. 沈阳药科大学学报， 2011 , 28 ( 1 ): 47 - 50 .

陶冉，王成章，孔振武．银杏叶类脂成分与聚戊烯醇的协同抑菌作用 [J]. 中国实验方剂学杂志， 2013 , 19 ( 17 ): 203 - 210 .

刘晓宇，陈旭冰，陈光勇． β-石竹烯及其衍生物的生物活性与合成研究进展 [J]. 林产化学与工业， 2012 , 32 ( 1 ): 104 - 110 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Optimization of Inclusion Process of Volatile Oil from Acori Tatarinowii Rhizoma and Hydroxypropyl--cyclodextrin by Central Composite Design-response Surface Methodology

Chemical Components of Essential Oils from and Their Antioxidant Activity

GC-MS Analysis of Effect of Different Processing Conditions on Irritation of Magnoliae Officinalis Cortex

Volatile Oil of Linderae Radix Induces Apoptosis and Autophagy of Gastric Cancer AGS Cells via AMPK/mTOR Signaling Pathway

Analysis of Quality Value Transmitting of Volatile Oil in Bran-processed Atractylodes lancea and Its Standard Decoction with Different Physicochemical Forms

Related Author

WANG Yue-liang

Chen Kai

LI Hui

ZHANG Xiao-li

LIU Yu-ling

WANG Jia-qi

LIU Wen-cong

XU Hai-tang

Related Institution

State Key Laboratory of Southwestern Chinese Medicine Resources， Chengdu University of Traditional Chinese Medicine

College of Pharmacy， Heilongjiang University of Chinese Medicine

Basic Medical College， Heilongjiang University of Chinese Medicine

Hubei Research Center of Chinese Materia Medica Processing Engineering and Technology， School of Pharmacy，Hubei University of Chinese Medicine

National Engineering Laboratory for Quality Control Technology of Chinese Materia Medica， Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰