Quality Evaluation of Standard Decoction of Zingiberis Rhizoma

Liang-liang GAO; Yan-lin ZHANG; Peng ZHANG; Xi-wen LI; Shi-lin CHEN; Qi LI; Zi-quan FAN; Dan-dan WANG; Yi SUN

doi:10.13422/j.cnki.syfjx.20190317

您当前的位置：

首页 >

文章列表页 >

Quality Evaluation of Standard Decoction of Zingiberis Rhizoma

Resource and Quality Evaluation | 更新时间：2021-02-09

- Quality Evaluation of Standard Decoction of Zingiberis Rhizoma
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 5, Pages: 161-166(2019)
- 作者机构：
  
  1.中国中医科学院　中药研究所，北京　 100700；
  2.安徽科技学院　生命与健康科学学院，安徽　凤阳　 233100；
  3.天津科技大学　生物工程学院，天津　 300457；
  4.上海市药材有限公司，上海　 200002；
  5.沃特世科技（上海）有限公司，上海　 201206
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190317
  CLC： R284.1;R289;R22;R2-031
- Received：18 May 2018，
  
  Published Online：16 November 2018，
  
  Published：05 March 2019
- 稿件说明：
移动端阅览
Liang-liang GAO, Yan-lin ZHANG, Peng ZHANG, et al. Quality Evaluation of Standard Decoction of Zingiberis Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 161-166.
DOI：

Liang-liang GAO, Yan-lin ZHANG, Peng ZHANG, et al. Quality Evaluation of Standard Decoction of Zingiberis Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 161-166. DOI： 10.13422/j.cnki.syfjx.20190317.

摘要

目的：

建立干姜饮片标准汤剂的质量控制方法。

方法：

对不同产地药材进行DNA条形码基原鉴定；根据中药饮片标准汤剂制备原则，将鉴定为干姜的饮片制备成标准汤剂并进行分析，同时对提取方法、分析方法进行方法学验证，计算出膏率和6-姜辣素的转移率等参数。建立干姜饮片标准汤剂的质量标准；采用UPLC-Q-TOF-MS对主要色谱峰进行结构确认，明确干姜标准汤剂中的主要化学成分。

结果：

所有药材经鉴定均为干姜(

Zingiber offcinale

)，在该文所建立的制备条件下，6-姜辣素的标准曲线为

＝661.56

＋2.493 3(

＝0.999 3)，精密度试验测得RSD为0.5%，表明仪器精密度良好。重复性试验测得RSD为0.3%，结果表明该方法重复性良好。稳定性试验测得RSD为0.4%，结果表明供试品溶液在24h内稳定性良好。加样回收率为97.2%，RSD为0.6%，表明该方法准确可靠。转移率范围为31.8%～57.4%，出膏率范围为9.6%～23.1%，12批干姜饮片标准汤剂指纹图谱相似度

90.0%。

结论：

建立的系统评价干姜标准汤剂的质量评价方法稳定可行，同时建立了规范的干姜标准汤剂制备方法及其质量评价体系。

Abstract

Objective:

To establish the quality control methods for the standard decoction of Zingiberis Rhizoma.

Method:

DNA barcode primitives were identified for the medicinal materials from different origins; according to the standard of Chinese herbal medicine decoction preparation principle

the identified Zingiberis Rhizoma was prepared into standard decoction for analysis. Meanwhile

the extraction method and analysis method were validated from methodologies

and the transfer rate of 6-gingerol as well as the extraction rate of standard decoction of Zingiberis Rhizoma were calculated. In addition

the quality standard of standard decoction of Zingiberis Rhizoma was also established based. The structures of main chromatographic peaks were identified by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to clarify the main chemical constituents in the standard decoction of Zingiberis Rhizoma.

Result:

All the samples were identified as Zingiberis Rhizoma. Under the conditions established in this paper

the standard curve of 6-gingerol was

=661.56

+ 2.493 3(

=0.999 3)

and the RSD was 0.5%in precision test

indicating that the instrument precision was good. The repeatability test showed that the RSD was 0.3%

indicating that the method had good repeatability. The stability test showed that the RSD was 0.4%

indicating that the test solution had good stability within 24h. The recovery rate was 97.2%and the RSD was 0.6%

indicating that the method was accurate and reliable. 6-gingerol's transfer rate ranged from 31.8%to 57.4%and the extraction rate was within the range of 9.6%-23.1%. The fingerprint similarity of 12 batches of Zingiberis Rhizoma standard decoction was

90%.

Conclusion:

The established quality control method for Zingiberis Rhizoma was stable and feasible; meanwhile

the standard preparation method for Zingiberis Rhizoma and its quality evaluation system were also established in this study.

关键词

Keywords

references

鲁文静，周密，梁宗锁．猪苓药材质量影响因素及质量评价的研究进展 [J]. 中国实验方剂学杂志， 2013 , 19 ( 17 ): 366 - 370 .

国家药典委员会．中华人民共和国药典．一部 [M]. 北京：中国医药科技出版社， 2015 ： 14 .

孙凤娇，李振麟，钱士辉，等．干姜化学成分和药理作用研究进展 [J]. 中国野生植物资源， 2015 , 34 ( 3 ): 34 - 37 .

崔婉华，王彦志，李泽之．干姜化学成分的分离鉴定 [J]. 中药材， 2018 , 41 ( 2 ): 334 - 337 .

QIAO X , LIN X H , JI S , et al . Global profiling and novel structure discovery using multiple neutral loss/precursor ion scanning combined with substructure recognition and statistical analysis (MNPSS): characterization of terpene-conjugated curcuminoids in curcuma longa as a case study [J]. Anal Chem , 2016 , 88 ( 1 ): 703 - 710 .

叶刚飒，余书洪，杨卫芳，等．生姜的有效成分与药理作用研究进展 [J]. 浙江树人大学学报：自然科学版， 2011 , 11 ( 3 ): 24 - 27 .

董艳，姚魁武，王阶．辨姜及其炮制品药理和临床运用特点 [J]. 中国中药杂志， 2018 , 43 ( 10 ): 2020 - 2024 .

吴智高．浅谈中药制剂的工艺与质量 [J]. 海峡药学， 2008 , 20 ( 12 ): 87 - 88 .

陈士林，刘安，李琦，等．中药饮片标准汤剂研究策略 [J]. 中国中药杂志， 2016 , 41 ( 8 ): 1367 - 1375 .

陈士林，姚辉，韩建萍，等．中药材DNA条形码分子鉴定指导原则 [J]. 中国中药杂志， 2013 , 38 ( 2 ): 141 - 148 .

祝明，陈碧莲，石上梅．中药指纹图谱技术在中国药典2015年版一部中的应用 [J]. 中国现代应用药学， 2016 , 33 ( 5 ): 611 - 614 .

张鹏，邬兰，李西文，等．人参饮片标准汤剂的评价及应用探讨 [J]. 中国实验方剂学杂志， 2017 , 23 ( 7 ): 2 - 11 .

于小红，赵嵘，代云桃，等．党参标准汤剂质量评价的建立 [J]. 中国实验方剂学杂志， 2017 , 23 ( 7 ): 24 - 29 .

李琦，章军，崔文金，等．黄芩饮片标准汤剂的制备和质量标准评价 [J]. 中国实验方剂学杂志， 2017 , 23 ( 7 ): 36 - 40 .

焦梦姣，邓哲，章军，等．含挥发性成分中药饮片标准汤剂的制备和质量标准研究——以牡丹皮为例 [J]. 中国中药杂志， 2018 , 43 ( 5 ): 891 - 896 .

徐姣，赵嵘，代云桃，等．中药饮片标准汤剂的质量评价案例——连翘 [J]. 中国中药杂志， 2018 , 43 ( 5 ): 868 - 872 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Quality Evaluation of Standard Decoction of Forsythiae Fructus

Quality Evaluation System of Standard Decoction of Puerariae Thomsonii Radix

HPLC Characteristic Fingerprint of Jiagasong Decoction

Quality Evaluation of Standard Decoction of Gardeniae Fructus

Quality Evaluation of Standard Decoction of Codonopsis Radix

Related Author

CAO Jing-ya

LI Xiao

SONG Meng-jiao

WEI Yue

ZHANG Li-xian

LI Zhi-ning

LI Zi-hong

CHEN Ling

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

Tianjin University of Traditional Chinese Medicine

Modern Research Center for Traditional Chinese Medicine，Shanxi University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰