Angiogenesis Activity of Morindae Officinalis Radix from Different Origins Based on Zebrafish Model

Zhi-fang RAN; Xiao-tong YANG; Zi-xin XU; Qing-xiu HAO; Jie ZHOU; Lan-ping GUO; Yi YU

doi:10.13422/j.cnki.syfjx.20190318

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Angiogenesis Activity of Morindae Officinalis Radix from Different Origins Based on Zebrafish Model

Resource and Quality Evaluation | 更新时间：2021-02-09

- Angiogenesis Activity of Morindae Officinalis Radix from Different Origins Based on Zebrafish Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 4, Pages: 175-179(2019)
- 作者机构：
  
  1.山东中医药大学　药学院，济南　 250355；
  2.济南大学　生物科学与技术学院，济南　 250022；
  3.中国中医科学院　中药资源中心　无限极中草药品质研究联合实验室，北京　 100700；
  4.无限极(中国)有限公司，广州　 510627
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190318
  CLC： R284.1;R285.5;R289;R22
- Received：07 June 2018，
  
  Published Online：16 November 2018，
  
  Published：20 February 2019
- 稿件说明：
移动端阅览
Zhi-fang RAN, Xiao-tong YANG, Zi-xin XU, et al. Angiogenesis Activity of Morindae Officinalis Radix from Different Origins Based on Zebrafish Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 175-179.
DOI：

Zhi-fang RAN, Xiao-tong YANG, Zi-xin XU, et al. Angiogenesis Activity of Morindae Officinalis Radix from Different Origins Based on Zebrafish Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 175-179. DOI： 10.13422/j.cnki.syfjx.20190318.

摘要

目的：

通过研究不同产地巴戟天提取物对斑马鱼破损节间血管生长的影响，探讨不同产地巴戟天的地域性差异。

方法：

建立PTK787诱导的节间血管损伤斑马鱼模型，研究来自广东、广西和福建等12个不同产地巴戟天提取物对斑马鱼节间血管生长的影响，检测给药组斑马鱼节间血管生成数、生成长度，计算血管相对生成率，并在主成分分析的基础上以血管相对生成率为指标，采用SPSS 17.0统计软件对实验数据进行聚类分析。

结果：

巴戟天提取物对斑马鱼破损节间血管的生长具有修复促进作用，且广东高要、广东郁南等地巴戟天提取物促斑马鱼血管生长效果明显优于其他产区，节间血管生成数平均达到9条，生成长度平均在448～504 μm，相对生成率达7.9%～8.86%。聚类分析结果显示，12个不同产地巴戟天被聚为四大类，广东德庆与广东高要，广西苍梧与广西岑溪，广东郁南与广西苍梧等地理位置相近的产地均能较好的聚到一起。

结论：

不同产地巴戟天在促血管生成作用上存在明显的地域性差异，相关研究为巴戟天道地药材的质量评价提供基础数据。

Abstract

Objective:

To study the effect of Morindae Officinalis Radixe extract from different origins on the vascular growth of damaged internodes of zebra fish

so as to discuss regional differences of Morindae Officinalis Radix from different origins.

Method:

A zebra fish model of internode vascular injury induced by PTK787 was established to study the effect of Morindae Officinalis Radix extract from Guangdong

Guangxi and Fujian on the growth of internode vascular in zebra fish

and detect the number of internode angiogenesis in zebra fish treated with PTK787.On the basis of principal component analysis

relative angiogenesis rate was taken as the index

and the experimental data was analyzed by SPSS 17.0 statistical software.

Result:

The extract of Morindae Officinalis Radix had a repairing effect on the growth of damaged internode blood vessels of zebra fish

and the extracts of Morindae Officinalis Radix from Guangdong Gaoyao

and Guangdong Yunan

were better than those from other producing areas. The average number of internode angiogenesis was 9

the average length of angiogenesis was 448-504 μm

and the relative rate of angiogenesis was between 7.9% and 8.86%. The results of cluster analysis showed that Morindae Officinalis Radix of 12 different origins were classified into four categories

Guangdong Deqing

and Guangdong Gaoyao

Guangxi Cangwu and Guangxi Cenxi

and Guangdong Yunan

and Guangxi Cangwu could be better clustered.

Conclusion:

There are obvious regional differences in the pro-angiogenic effect of Morindae Officinalis Radix from different origins. Relevant studies provide basic data for the quality assessment of Morindae Officinalis Radix medicinal materials.

关键词

Keywords

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