Effect of Huangqi Guizhi Wuwu Tang on Angiogenesis in Osteoarthritis

Le ZHAO; Yan-yan LI; Yong-hui WANG; Xiao-jun DONG; Ran ZHOU; Ruo-nan ZHANG; Li-wei SUN

doi:10.13422/j.cnki.syfjx.20190338

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Effect of Huangqi Guizhi Wuwu Tang on Angiogenesis in Osteoarthritis

Pharmacology | 更新时间：2021-02-09

- Effect of Huangqi Guizhi Wuwu Tang on Angiogenesis in Osteoarthritis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 3, Pages: 87-93(2019)
- 作者机构：
  
  1.成都中医药大学，成都　 611137
  2.山西中医药大学，太原　 030024
  3.遵义医药高等专科学校，贵州　遵义　 563006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190338
  CLC： R2-0;R22;R285.5
- Received：12 July 2018，
  
  Published Online：16 November 2018，
  
  Published：05 February 2019
- 稿件说明：
移动端阅览
Le ZHAO, Yan-yan LI, Yong-hui WANG, et al. Effect of Huangqi Guizhi Wuwu Tang on Angiogenesis in Osteoarthritis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(3): 87-93.
DOI：

Le ZHAO, Yan-yan LI, Yong-hui WANG, et al. Effect of Huangqi Guizhi Wuwu Tang on Angiogenesis in Osteoarthritis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(3): 87-93. DOI： 10.13422/j.cnki.syfjx.20190338.

摘要

目的:

研究黄芪桂枝五物汤对阳虚寒凝骨关节炎(osteoarthritis

OA)模型大鼠血管内皮生长因子(VEGF)及相关因子的影响。

方法:

60只SPF级SD大鼠随机分为正常组，模型组，塞来昔布组(20.82 mg·kg

-1

)，黄芪桂枝五物汤低、中、高剂量组(3.24，6.48，12.96 g·kg

-1

)。除正常组外，其余各组通过冷固法复合寒冷环境刺激42 d，复制OA模型。造模后，给药组分别灌胃相应药物，正常组和模型组给同等剂量蒸馏水，每天1次，连续28 d。末次给药24 h后，酶联免疫吸附测定(ELISA)检测血清中VEGF，前列腺素E

(PGE

)，转化生长因子

(TGF-

)水平；免疫组化染色检测左膝关节滑膜和软骨内VEGF表达；实时荧光定量聚合酶链式反应(Real-time PCR)检测右膝关节滑膜白细胞介素-17(IL-17)和VEGF mRNA表达。

结果:

与正常组比较，模型组血清、软骨和滑膜组织中VEGF表达均显著性升高(

0.01)，同时，血清中PGE

和TGF-

，滑膜组织IL-17水平均显著升高(

0.01)；与模型组比较，各用药组血清、软骨和滑膜组织中VEGF表达均显著性降低(

0.05)，同时，血清中PGE

和TGF-

水平，滑膜组织中IL-17水平亦显著降低(

0.05)；各用药组间比较，黄芪桂枝五物汤高剂量组膝关节滑膜组织IL-17较塞来昔布组升高(

0.05)，黄芪桂枝五物汤中、高剂量组VEGF较塞来昔布组显著升高(

0.01)，其余各组间比较均无显著性差异。

结论:

黄芪桂枝五物汤可能通过减轻骨关节炎大鼠血管新生相关细胞因子PGE

和TGF-

等的表达而作用于VEGF，从而抑制膝骨关节处血管新生，减轻软骨损伤。

Abstract

Objective:

To study the effect of Huangqi Guizhi Wuwu Tang on angiogenesis of osteoarthritis with Yang deficiency and cold coagulation.

Method:

Totally 60 female SD rats were randomly divided into control group

model group

celecoxib group (20.82 mg·kg

-1

) and low

medium

high-dose Huangqi Guizhi Wuwu Tang groups (3.24

6.48

12.96 g·kg

-1

). All groups

except for control group

were involved in duplicating the osteoarthritis(OA) model through frozen and knee fixation

as well as 42-day cold environmental stimulation. After modeling

all drug-group rats were respectively administrated with corresponding drugs for 28 days

once a day. Meanwhile

control group and model group were given equivalent distilled water by gavage. 24 hours after the last gavage

vascular endothelial growth factor (VEGF)

prostaglandin E

(PGE

) and transforming growth factor-

(TGF-

) in serum were detected with enzyme linked immunosorbent assay(ELISA) method

VEGF expressions in cartilage and synovial with immunohistochemical method

and interleukin-17(IL-17) and VEGF levels in synovial with Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR).

Result:

Compared with normal group

the expression of VEGF in serum

cartilage and synovial were significantly increased (

0.01)

and PGE

and TGF-

expressions

IL-17 level were increased significantly (

0.01) as well. Compared with model group

VEGF in serum

cartilage and synovial were significantly decreased in the 4 drug groups. Meanwhile

PGE

TGF-

and IL-17 level were decreased significantly (

0.05). Among drug groups

IL-17 of high-dose group in synovial was higher than that of celecoxib group (

0.05)

and VEGF of medium and high-dose Huangqi Guizhi Wuwu Tang groups in synovial was higher than that of celecoxib group (

0.05). There was no significant difference among other drug groups.

Conclusion:

Huangqi Guizhi Wuwu Tang has an effect in suppressing angiogenesis of knee and alleviate cartilage lesion by regulating VEGF and its upstream cytokines PGE

and TGF-

关键词

Keywords

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