Effect of Shenling Baizhu San on Expressions of NLPR3, NLPR6 Protein and Related Inflammatory Factors in Mice with Ulcerative Colitis

Jia-jun ZHANG; Jia-xi SHI; Juan HUANG; Xu-guang SHI; Cui-ying LIU; Qi WANG

doi:10.13422/j.cnki.syfjx.20190401

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Effect of Shenling Baizhu San on Expressions of NLPR3, NLPR6 Protein and Related Inflammatory Factors in Mice with Ulcerative Colitis

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- Effect of Shenling Baizhu San on Expressions of NLPR3, NLPR6 Protein and Related Inflammatory Factors in Mice with Ulcerative Colitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 4, Pages: 36-41(2019)
- 作者机构：
  
  1.广州中医药大学　中药学院，广州　 510006；
  2.广州中医药大学　第一临床医学院，广州　 510006；
  3.佛山市中医院，广东　佛山　 528000；
  4.广州中医药大学　临床药理研究所，广州　 510405
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190401
  CLC： R2-0;R22;R285.5;R289
- Received：16 July 2018，
  
  Published Online：06 November 2018，
  
  Published：20 February 2019
- 稿件说明：
移动端阅览
Jia-jun ZHANG, Jia-xi SHI, Juan HUANG, et al. Effect of Shenling Baizhu San on Expressions of NLPR3, NLPR6 Protein and Related Inflammatory Factors in Mice with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 36-41.
DOI：

Jia-jun ZHANG, Jia-xi SHI, Juan HUANG, et al. Effect of Shenling Baizhu San on Expressions of NLPR3, NLPR6 Protein and Related Inflammatory Factors in Mice with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 36-41. DOI： 10.13422/j.cnki.syfjx.20190401.

摘要

目的：

探讨参苓白术散对3%葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠的治疗作用及相关的作用机制。

方法：

60只雄性SPF级C57BL/6小鼠随机分为正常组，模型组，柳氮磺吡啶组(0.52 g·kg

－1

)，参苓白术散高、中、低(31.2，15.6，7.8 g·kg

－1

)剂量组。除正常组外小鼠饮用3%葡聚糖硫酸钠蒸馏水1周造成UC模型。造模结束后每日给药1次，正常组和模型组按20 mL·kg

－1

给予0.9%生理盐水，共14 d。每日观察小鼠体质量、粪便性状及隐血情况并计成疾病活动指数(DAI)评分。给药结束后取血收集血清，取小鼠结肠称质量测量长度并制作病理切片。酶联免疫吸附测定(ELISA)检测血清白细胞介素-18(IL-18)，IL-1

含量；苏木素-伊红(HE)及阿利新蓝-过碘酸雪夫氏(AB/PAS)染色观察结肠病理情况；蛋白免疫印迹法(Western blot)检测结肠核苷酸结合寡聚化结构域样受体3(NLPR3)，NLPR6蛋白表达水平。

结果：

与正常组比较，模型组小鼠DAI评分显著升高(

0.01)，结肠质量增加、长度变短(

0.01)；结肠黏膜病理受损严重；血清中IL-1

含量明显增高(

0.05)；NLRP3蛋白表达升高，NLRP6表达降低(

0.01)。给药后，与模型组比较，SASP组与高剂量组DAI评分降低(

0.05)，结肠质量、长度、病理情况均改善；血清中IL-1

含量降低(

0.05)；NLRP3蛋白表达降低(

0.01)，NLRP6蛋白表达上调(

0.05，

0.01)。

结论：

参苓白术散有治疗DSS诱导UC小鼠的作用，其作用机制可能与其调节NLRP3，NLRP6蛋白及相关炎症因子，从而减轻肠道炎症反应，缓解肠黏膜损伤有关。

Abstract

Objective:

To investigate the therapeutic effect and related mechanism of Shenling Baizhu San on 3%dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice.

Method:

Sixty male SPF C57BL/6 mice were randomly divided into control group

salazosulfapyridine (SASP

0.52 g·kg

-1

)

and low

medium and high-dose (31.2

15.6

7.8 g·kg

-1

) Shenling Baizhu San groups. Except for the control group

mice in the other groups were given distilled water containing 3%dextran sulfate sodium salt for a week to establish UC models. The drug was administered once a day for 14 days. The normal group and the model group were administered with 0.9%physiological saline at 20 mL·kg

-1

. The mice's body weight

fecal traits

and occult blood were observed daily

and the disease activity index (DAI) was scored. After the end of the administration

the blood was collected

mice colons were collected

weighed and measured for length

and pathological sections were prepared. Enzyme-linked immunosorbent assay (ELISA) kit was used to detect the serum levels of interleukin-18 (IL-18) and IL-1

in mice; htoxylin eosin (HE) and alixin blue/schiff periodic acid shiff(AB/PAS) staining were used to observe the pathological changes of colon tissues; Western blot was used to detect the colon tissue of mice nucleotide binding oligomerization domain-like receptor 3 (NLPR3)

NLPR6 protein expression levels.

Result:

Compared with the normal group

the DAI score of the model group was increased (

0.01)

the colon weight was increased

and the length was shortened (

0.01); the pathological damage of the colonic mucosa was severe; the serum IL-1

content increased (

0.05); NLRP3 protein expression increased

while NLRP6 expression decreased (

0.01). Compared with the model group

the DAI scores of the SASP group and the high-dose group were decreased (

0.05)

the colon weight

length

and pathological conditions were improved; the serum IL-1

concentration was decreased (

0.05); NLRP3 protein expression decreased (

0.01)

and NLRP6 expression was up-regulated (

0.05

0.01).

Conclusion:

Shenling Baizhu San has the effect in treating DSS-induced UC mice

which may be related to the regulation of NLRP3

NLRP6 protein and related inflammatory factors

so as to reduce intestinal inflammation and alleviate intestinal mucosal damage.

关键词

Keywords

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