Separation Identification of Chemical Constituents of Lysimachia capillipes

Kun-yan ZHANG; Ting HONG; Yuan QIAN; Min-yu WU; Guang-wen ZHANG; Dong WANG; Yi-sheng YANG

doi:10.13422/j.cnki.syfjx.20190413

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Separation Identification of Chemical Constituents of Lysimachia capillipes

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- Separation Identification of Chemical Constituents of Lysimachia capillipes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 6, Pages: 156-162(2019)
- 作者机构：
  
  1.江西中医药大学　药学院，南昌　 330004；
  2.江西省药品检验检测研究院　江西省药品与医疗器械质量工程技术研究中心，南昌　 330029；
  3.江西省食品药品监督管理局，南昌　 330029；
  4.江西省食品检验检测研究院　江西国家果蔬产品及加工食品质量监督检验中心，南昌　 330001
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190413
  CLC： R284.2;R22;R2-03;R289
- Received：24 May 2018，
  
  Published Online：11 May 2018，
  
  Published：20 March 2019
- 稿件说明：
移动端阅览
Kun-yan ZHANG, Ting HONG, Yuan QIAN, et al. Separation Identification of Chemical Constituents of Lysimachia capillipes[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 156-162.
DOI：

Kun-yan ZHANG, Ting HONG, Yuan QIAN, et al. Separation Identification of Chemical Constituents of Lysimachia capillipes[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 156-162. DOI： 10.13422/j.cnki.syfjx.20190413.

摘要

目的：

对细梗香草正丁醇部位进行系统的化学成分研究。

方法：

取细梗香草药材粉碎，用70%乙醇回流提取，减压回收溶剂得浸膏，浸膏经水溶解后，用正丁醇萃取，得到正丁醇部位，采用大孔树脂柱，中压ODS柱色谱，硅胶柱色谱，LH-20型羟丙基葡聚糖凝胶(Sephadex LH-20)柱色谱和制备高效液相色谱等技术手段进行分离纯化，分离得到单体化合物，并经波谱数据分析和文献数据鉴定化合物的结构。

结果：

从细梗香草正丁醇提取物中分离得到15个化合物，其中6个皂苷类和9个黄酮苷类化合物，分别鉴定为细梗香草皂苷B(

)，细梗香草皂苷C(

)，kaempferol-3-

-xylopyranosyl(1→3)-[4-

-coumaroyl-

-rhamnopyranosyl(1→2)][

-glucopyranosyl(1→6)]-

-galactopyranoside-7-

-rhamnopyranoside(

)，kaempferol-3-

-{[

-xylopyranosyl(1→3)-

-rhamnopyranosyl(1→6)][

-rhamnopyranosyl(1→2)]}-

-3-trans-

-coumaroylgalactopyranoside(

)，细梗香草皂苷K(

)，3

-rhamnopyranosyl-(1→2)-

-glucopyranosyl-(1→4)-[

-glucopyranosyl-(1→2)]-

-arabinopyranosyl}-16

-hydroxyolean-28，13

-olide (

)，细梗香草皂苷I(

)，quercetin-3-

-(2″，6″-di-

-rhamnopyranosyl)-

-galactopyranoside(

)，kaempferol-3-

-{[

-xylopyranosyl(1→3)-

-rhamnopyranosyl(1→6)][

-rhamnopyranosyl-(1→2)]}-

-galactopyranoside(

)，kaempferol-3-

-[2-glucopyranosyl(1→3)rhamnopyranosyl-6-rhamnopyranosyl]-

-galactopyranoside(

)，kaempferol-3-

-rhamnopyranosy-(1→2)-[

-rhamnopyranosy-(1→6)]-

-galactopyranoside(

)，capilliposide I(

)，kaempferol-3-

-{(

-glucopyranosyl-(1→3)-[4-

-coumaroyl)]-α-

-rhamnopyranosyl-(1→6)-(

-galactopyranoside)}-7-

-rhamnopyranoside (

)，kaempferol-3-

-{[

-glucopyranosyl(1→3)]-4-

-coumaroyl)}-

-rhamnopyranosyl(1→6)-

-glucopyranoside-7-

(4-

-acetyl)-

-rhamnopyranoside (

)，(3

，20

，23

，24

)-3，20，23，24，25，29-hexahydroxydammaran-21-oic acid-21，23-lactone 3-

-glucopyranosyl-(1→6)-

-gluco-pyranoside(

)。

结论：

化合物

3，4，6，9，10，13～15

为首次在该植物中分离得到。

Abstract

Objective:

To systemically study the chemical constituents of

-butanol fraction from

Lysimachia capillipes

Method:

The whole plant of

capillipes

was crushed into power

extracted by 70%methanol

concentrated under reduced pressure

and then its

-butanol extract was obtained by fractional extraction. The compounds from

-butanol fraction were isolated and purified by macroporous resin column chromatography

medium pressure ODS

silica gel

Sephadex LH-20 and preparative HPLC. Their structures were identified on the basis of spectral analysis and comparison with literature data.

Result:

Fifteen compounds including 6 saponins and 9 flavonoid glycosides were isolated from

capillipes

and were identified as ascapilliposide B(

) and capilliposide C(

)

kaempferol-3-

-xylopyranosyl(1→3)-[4-

-coumaroyl-

-rhamnopyranosyl(1→2)][

-glucopyranosyl(1→6)]-

-galactopyranoside-7-

-rhamnopyranoside(

)

kaempferol-3-

-{[

-xylopyranosyl(1→3)-

-rhamnopyranosyl(1→6)][

-rhamnopyranosyl(1→2)]}-

-3-trans-

-coumaroylgalactopyranoside (

)

capilliposide K (

)

-rhamnopyranosyl-(1→2)-

-glucopyranosyl-(1→4)-[

-glucopyranosyl-(1→2)]-

-arabinopyranosyl)}-16

-hydroxyolean-28

-olide (

)

capilliposide I(

)

quercetin-3-

-(2″

6″-di-

-rhamnopyranosyl)-

-galactopyranoside(

)

kaempferol-3-

-{[

-xylopyranosyl(1→3)-

-rhamnopyranosyl(1→6)][

-rhamnopyranosyl-(1→2)]}-

-galactopyranoside(

)

kaempferol-3-

-[2-glucopyranosyl(1→3)rhamnopyranosyl-6-rhamnopyranosyl]-

-galactopyranoside(

)

kaempferol-3-

-rhamnopyranosy-(1→2)-[

-rhamnopyranosy-(1→6)]-

-galactopyranoside(

)

capilliposide I(

)

kaempferol-3-

-{(

-glucopyranosyl-(1→3)-[4-

-coumaroyl)]-α-

-rhamnopyranosyl-(1→6)-(

-galactopyranoside)}-7-

-rhamnopyranoside (

)

kaempferol-3-

-{[

-glucopyranosyl(1→3)]-4-

-coumaroyl)}-

-rhamnopyranosyl(1→6)-

-glucopyranoside-7-

(4-

-acetyl)-

-rhamnopyranoside (

)

and (3

)-3

29-hexahydroxydammaran-21-oic acid-21

23-lactone 3-

-glucopyranosyl-(1→6)-

-gluco-pyranoside(

Conclusion:

The compounds

13-15

were isolated from this plant for the first time.

关键词

Keywords

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