Mechanism of Nephrotoxicity of Aloe Emodin in Mice

Yan-qiao LI; Wan-yi HUANG; Yu-sheng LIANG; Yu LUO; Qing JIANG; Yong ZENG; Ping WANG; Xian-li MENG

doi:10.13422/j.cnki.syfjx.20190424

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Mechanism of Nephrotoxicity of Aloe Emodin in Mice

Topic on Toxicity of Anthraquinones in Rhei Radix et Rhizoma | 更新时间：2021-02-09

- Mechanism of Nephrotoxicity of Aloe Emodin in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 11, Pages: 48-53(2019)
- 作者机构：
  
  成都中医药大学，成都　 611137
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190424
  CLC： R22; R242; R2-031; R285.5
- Received：06 August 2018，
  
  Published Online：05 November 2018，
  
  Published：05 June 2019
- 稿件说明：
移动端阅览
Yan-qiao LI, Wan-yi HUANG, Yu-sheng LIANG, et al. Mechanism of Nephrotoxicity of Aloe Emodin in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 48-53.
DOI：

Yan-qiao LI, Wan-yi HUANG, Yu-sheng LIANG, et al. Mechanism of Nephrotoxicity of Aloe Emodin in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 48-53. DOI： 10.13422/j.cnki.syfjx.20190424.

摘要

目的：

研究长期给予不同剂量的芦荟大黄素导致小鼠肾毒性，并探讨其毒性机制。

方法：

将30只昆明种小鼠随机分为雌雄空白组、雌雄芦荟大黄素低、高剂量组(0.8，1.6 g·kg

－1

)。芦荟大黄素各剂量组连续灌胃给药11周，每日早晚各1次。采用生化试剂盒检测小鼠血清尿素氮(urea nitrogen，BUN)，肌酐(creatinine，SCr)，超氧化物歧化酶(superoxide dismustase，SOD)和丙二醛(malondialdehyde，MDA)含量，肾脏总谷胱甘肽(total glutathione，T-GSH)/氧化性谷胱甘肽试剂盒(oxidized glutathione，GSSG)和谷胱甘肽过氧化物酶(glutathione peroxidase，GSH-Px)水平；酶联免疫吸附试验(ELISA)检测血清中肿瘤坏死因子-

(tumor necrosis factor alpha，TNF-

)和白细胞介素-6(interleukins-6，IL-6)水平；苏木素-伊红(HE)染色检测肾脏病理变化；免疫组化检测肾脏半胱氨酸蛋白酶-3(cysteine aspartic acid specific protease-3，Caspase-3)和转化生长因子-

(transforming growth factor-

，TGF-

)蛋白表达。

结果：

与同性别空白组比较，芦荟大黄素高剂量组雌、雄小鼠血清中BUN含量升高(

0.05，

0.01)，芦荟大黄素高剂量组雄性小鼠血清中SCr含量升高(

0.05)，芦荟大黄素低剂量组肾小管轻度损伤，高剂量肾小管和肾小球中度损伤；与空白组比较，芦荟大黄素高剂量组雌、雄小鼠血清MDA含量升高(

0.05)，SOD活性降低(

0.05)，芦荟大黄素高剂量组雌雄小鼠肾脏中GSH/GSSG含量降低(

0.05)，雄性小鼠Caspase-3蛋白表达增加(

0.05)；与空白组比较，芦荟大黄素高剂量组雄性小鼠TNF-

和IL-6含量升高(

0.05)，芦荟大黄素低、高剂量组雌雄小鼠肾脏TGF-

蛋白表达均增加(

0.05)。

结论：

芦荟大黄素1.6 g·kg

－1

给药11周，对小鼠肾脏有毒性作用，其机制与机体氧化应激、细胞凋亡和TGF-

蛋白表达有关。

Abstract

Objective:

To study nephrotoxicity induced by long-term administration of different doses of aloe-emodin in mice

and explore its mechanism.

Method:

A total of 30 male and female Kunming mice were randomly divided into normal control group

and low-dose aloe-emodin group

high-dose aloe-emodin group (0.8

1.6 g·kg

-1

). Every dose of group was administered intragastrically for 11 weeks

twice daily. effect of serum urea nitrogen (BUN)

creatinine (SCr)

superoxide dismutase (SOD)

malondialdehyde (MDA)

Glutathione (GSH/GSSG) and Glutathione Peroxidase (GSH-Px) levels were detected by biochemical kits according to manufacturer's instruction. Enzyme-linked immune assay was used to determine serum tumor necrosis factor (TNF)-

and interleukins(IL)-6 levels. Hematoxylin eosin(HE) staining was used to detect renal pathological changes in kidney tissues

and cysteine aspartic acid specific protease(Caspase)-3 and transforming growth factor(TGF)-

proteins were determined by immunohistochemistry.

Result:

According to results

compared with normal control group

the levels of BUN and SCr in serum with high-dose aloe-emodin were increased. The renal tubules in low-dose group were mildly injured

while renal tubules and glomeruli of high-dose group were moderately damaged. Compared with normal control group

the level of SOD was significant decreased (

0.05)

MDA was increased (

0.05)

the levels of GSH/GSSG in kidneys of high-dose groups were decreased (

0.05). In high-dose group

the expression of Caspase-3 protein was increased in kidneys

especially in males (

0.05). Compared with normal control group

the levels of TNF-

and IL-6 were increased

the expression of TGF-

protein in kidneys was increased in low-dose and high-dose groups (

0.05).

Conclusion:

results show that 1.6 g·kg

-1

aloe-emodin was administered intragastrically for 11 weeks

which had toxic effects on kidney in mice. The mechanism may be related to oxidative stress

apoptosis and TGF-

protein expression.

关键词

Keywords

references

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