Effect of Yanghe Huayan Tang Combined with PI3K Inhibitor on Expressions of HER-2, PI3K and p-Akt in PI3K/Akt Pathway of High-expression HER-2 Breast Cancer Cell Lines

Nan WANG; Xiao-fei LIU; Yang ZHANG; Qing ZHU

doi:10.13422/j.cnki.syfjx.20190425

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Effect of Yanghe Huayan Tang Combined with PI3K Inhibitor on Expressions of HER-2, PI3K and p-Akt in PI3K/Akt Pathway of High-expression HER-2 Breast Cancer Cell Lines

Pharmacology | 更新时间：2021-02-09

- Effect of Yanghe Huayan Tang Combined with PI3K Inhibitor on Expressions of HER-2, PI3K and p-Akt in PI3K/Akt Pathway of High-expression HER-2 Breast Cancer Cell Lines
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 5, Pages: 98-104(2019)
- 作者机构：
  
  山东中医药大学　第一临床医学院，中医学院，济南　 250014
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190425
  CLC： R22;R242;R2-031;R285.5;R273
- Received：17 October 2018，
  
  Published Online：05 November 2018，
  
  Published：05 March 2019
- 稿件说明：
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Nan WANG, Xiao-fei LIU, Yang ZHANG, et al. Effect of Yanghe Huayan Tang Combined with PI3K Inhibitor on Expressions of HER-2, PI3K and p-Akt in PI3K/Akt Pathway of High-expression HER-2 Breast Cancer Cell Lines[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 98-104.
DOI：

Nan WANG, Xiao-fei LIU, Yang ZHANG, et al. Effect of Yanghe Huayan Tang Combined with PI3K Inhibitor on Expressions of HER-2, PI3K and p-Akt in PI3K/Akt Pathway of High-expression HER-2 Breast Cancer Cell Lines[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 98-104. DOI： 10.13422/j.cnki.syfjx.20190425.

摘要

目的：

观察阳和化岩汤与磷脂酰肌醇-3羟基激酶(PI3K)抑制剂LY294002对乳腺癌细胞株SK-BR-3[人表皮生长因子受体-2(HER-2)高表达型]PI3K/蛋白激酶B(Akt)信号通路中HER-2，PI3K，磷酸化蛋白激酶B(p-Akt)表达的影响，研究阳和化岩汤的干预机制。

方法：

制备阳和化岩汤肠吸收液，将乳腺癌细胞株SK-BR-3分为空白组，阳和化岩汤组，LY294002组，LY294002＋阳和化岩汤组，阳和化岩汤(125g·L

－1

)，LY294002(0.05g·L

－1

)，阳和化岩汤＋LY294002（阳和化岩汤125g·L

－1

，LY294002 0.05g·L

－1

），用药干预24h后通过免疫组化、蛋白免疫印迹法(Western blot)检测HER-2，PI3K，p-Akt蛋白表达，通过逆转录聚合酶链式反应(RT-PCR)检测HER-2，PI3K，p-AktmRNA表达。

结果：

与空白组比较，LY294002组，LY294002＋阳和化岩汤组均能抑制乳腺癌细胞株HER-2，PI3K，p-Akt蛋白及mRNA表达(

0.05)；阳和化岩汤组能抑制乳腺癌细胞株HER-2，p-Akt蛋白及mRNA表达(

0.05)，但对PI3K的抑制作用不明显；联用LY294002较单用LY294002具有进一步抑制PI3K表达的作用(

0.05)。与阳和化岩汤组，LY294002组比较，LY294002＋阳和化岩汤组能够明显抑制HER-2，PI3K，p-Akt蛋白及mRNA表达(

0.05)。

结论：

阳和化岩汤能够抑制乳腺癌脉管生成和侵袭，主要作用机制为通过干预PI3K/Akt通路，降低通路中HER-2，PI3K，p-Akt表达。

Abstract

Objective:

To investigate effect of Yanghe Huayan Tang and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 in intervening expressions of human epidermal growth factor receptor human epidermal growth factor receptor-2(HER-2)

PI3K and phosphorylated serine/threonine kinase (p-Akt)in PI3K/Akt pathway of breast cancer cell line SK-BR-3 (HER-2 high expression)

in vitro

and study intervention mechanism of Yanghe Huayan Tang.

Method:

Yanghe Huayan Tang intestinal absorption fluid was prepared

breast cancer cell strain SK-BR-3 was divided into blank group

Yanghe Huayan Tang group

LY294002 group

LY294002+ Yanghe Huayan Tang group. The concentration was respectively 125 g·L

-1

for Yanghe Huayan Tang

0.05 g·L

-1

for LY294002

125 g·L

-1

for Yanghe Huayan Tang+ LY294002.The expressions of HER-2

PI3K and p-Akt protein were detected by immunohistochemistry and Western blot after 24 h. The most appropriate and effective concentration was obtained through extensive experiments. The expressions of HER-2

PI3K and p-Akt were detected by reverse transcription polymerase chain reaction (RT-PCR).

Result:

Compared with blank group

LY294002 group

and LY294002+ Yanghe Huayan Tang group could inhibit protein and mRNA expressions of HER-2

PI3K

p-Akt in breast cancer cell lines(

0.05). Yanghe Huayan Tang group could inhibit expressions of HER-2

p-Akt and mRNA (

0.05) in breast cancer cell lines

but inhibitory effect on PI3K was not obvious; combination with LY294002 could further inhibit PI3K expression compared with single administration with LY294002 (

0.05). Compared with LY294002 group

LY294002+ Yanghe Huayan Tang group had a more obvious inhibitory effect. The combined administration of Yanghe Huayan Tang and LY294002 could inhibit protein and mRNA expressions of HER-2

PI3K

p-Akt significantly (

0.05).

Conclusion:

Yanghe Huayan Tang can inhibit angiogenesis and invasion of breast cancer. Its main mechanism is expressed by intervening PI3K/Akt pathway

reducing expressions of HER-2

PI3K and p-Akt in pathway.

关键词

Keywords

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