Effect of Sanshencao Essential Oil on Rat Model of Thromboangiitis Obliterans

Jing LIU; Shuo TIAN; Ming-san MIAO

doi:10.13422/j.cnki.syfjx.20190438

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Effect of Sanshencao Essential Oil on Rat Model of Thromboangiitis Obliterans

Topical Study of TCM for External Use | 更新时间：2021-02-09

- Effect of Sanshencao Essential Oil on Rat Model of Thromboangiitis Obliterans
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 4, Pages: 18-22(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190438
  CLC： R26;R285.5;R364.5
- Received：04 October 2018，
  
  Published Online：08 November 2018，
  
  Published：20 February 2019
- 稿件说明：
移动端阅览
Jing LIU, Shuo TIAN, Ming-san MIAO. Effect of Sanshencao Essential Oil on Rat Model of Thromboangiitis Obliterans[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 18-22.
DOI：

Jing LIU, Shuo TIAN, Ming-san MIAO. Effect of Sanshencao Essential Oil on Rat Model of Thromboangiitis Obliterans[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 18-22. DOI： 10.13422/j.cnki.syfjx.20190438.

摘要

目的：

观察三参草精油对大鼠血栓闭塞性脉管炎模型的疗效。

方法：

按体质量将Wistar雄性大鼠随机分为5组：假手术组，模型组，通塞脉片组(1.05 g·kg

－1

)，三参草精油大、小剂量组(0.125 mL·cm

－2

，2次/d；0.125 mL·cm

－2

，1 次/d)，每组10只。将10 g·L

－1

月桂酸钠溶液注入大鼠左下肢动脉造闭塞型脉管炎模型，假手术组于同位置注射等量的生理盐水。三参草精油大、小剂量直接涂抹患处，余下各组灌服相应药物或纯净水，持续10 d。末次给药1 h后，对各组动物病变程度分级；放射免疫法测血清血栓素B

(TXB

)，白细胞介素-1(IL-1)，6-酮-前列腺素(6-K-PGF

)，内皮素(ET)含量；苏木素-伊红(HE)染色观察大鼠左下肢股动脉的病理变化。

结果：

与假手术组比较，模型组患肢病变程度、范围、炎症和血栓明显增多(

0.05，

0.01)；与模型组比较，三参草精油大、小剂量组均可明显减轻患肢病变程度、范围、炎症和血栓(

0.05，

0.01)；降低血清中TXB

，ET，IL-1水平(

0.05，

0.01)，升高6-K-PGF

水平(

0.05，

0.01)。

结论：

三参草精油对大鼠血栓闭塞性脉管炎模型有较好的治疗作用，与其抗氧化、减轻血管壁炎症反应有关。

Abstract

Objective:

To observe the effect of Sanshencao essential oil on the model of thromboangiitis obliterans in rats.

Method:

Male Wistar rats were randomly divided into five groups according to body weight: sham operation group

model group

Tongsaimai group (1.05 g·kg

-1

)

large and small-dose Sanshencao essential oil groups (0.125 mL·cm

-2

twice a day

0.125 mL·cm

-2

once a day)

with 10 in each group. The model of occlusive vasculitis was established by injecting sodium laurate solution (10 g·L

-1

) into the left lower limb artery of rats. Sham operation group was injected with the same amount of normal saline at the same position. Large and small doses of Sanshencao essential oil were applied directly to the affected area

and the remaining groups were given corresponding drugs or pure water for 10 days. One hour after the last administration

the pathological changes were graded; the levels of thromboxane B

(TXB

)

interleukin-1 (IL-1)

6-oxo-prostaglandinF1

(6-K-PGF

)

endothelin (ET) were measured by radioimmunoassay. Hematoxylin-eosin(HE) staining was used to observe the pathological changes of the femoral arteries in the left lower limbs.

Result:

Compared with sham operation group

the degree

extent

inflammation and thrombosis of the affected limbs in model group increased significantly (

0.05

0.01). Compared with model group

large and small-dose Sanshencao essential oil could significantly alleviate the extent

inflammation and thrombosis of the affected limbs (

0.05

0.01)

decrease the levels of TXB

ET and IL-1 in serum (

0.05

0.01)

and increase the level of 6-K-PGF

(

0.05

0.01).

Conclusion:

Sanshencao essential oil has a good therapeutic effect on thrombosis angiitis obliterans model in rats

which related to its anti-oxidation and alleviating inflammation of vascular wall.

关键词

Keywords

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