Effect of Zingiberis Rhizoma Recens on Hemorrhoids Model in Rats and Mice

Xin LOU; Ming BAI; Shuo TIAN; Xiang-ping WEI; Ming-san MIAO

doi:10.13422/j.cnki.syfjx.20190440

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Effect of Zingiberis Rhizoma Recens on Hemorrhoids Model in Rats and Mice

Topical Study of TCM for External Use | 更新时间：2021-02-09

- Effect of Zingiberis Rhizoma Recens on Hemorrhoids Model in Rats and Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 4, Pages: 23-28(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190440
  CLC： R26;R285.5;R657.1+8
- Received：04 October 2018，
  
  Published Online：20 November 2018，
  
  Published：20 February 2019
- 稿件说明：
移动端阅览
Xin LOU, Ming BAI, Shuo TIAN, et al. Effect of Zingiberis Rhizoma Recens on Hemorrhoids Model in Rats and Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 23-28.
DOI：

Xin LOU, Ming BAI, Shuo TIAN, et al. Effect of Zingiberis Rhizoma Recens on Hemorrhoids Model in Rats and Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 23-28. DOI： 10.13422/j.cnki.syfjx.20190440.

摘要

目的：

探讨生姜外用对大、小鼠痔疮模型的疗效。

方法：

SPF级SD大鼠60只，分为空白组，模型组，马应龙麝香痔疮膏组(7.5 g·kg

－1

)，生姜糊大、小剂量组(10，5 g·kg

－1

)，采用生姜糊外敷涌泉穴方法，观察对由75%乙酸溶液0.05 mL注射于大鼠肛门外周皮下所致大鼠痔疮模型的影响，酶联免疫吸附测定(ELISA)检测血清白细胞介素-1

(IL-1

)，白细胞介素-6(IL-6)，肿瘤坏死因子-

(TNF-

)，一氧化氮(NO)水平，苏木素-伊红(HE)染色评价直肠组织病理形态的改变；SPF级KM小鼠60只，分为空白组，模型组，马应龙麝香痔疮膏组(7.5 g·kg

－1

)，生姜精油大、小剂量组(0.06，0.03 mL，每天3次)，采用生姜精油外涂肛周方法，观察对由20%乙酸溶液0.05 mL内推入小鼠肛门(保持1 min)所致小鼠痔疮模型的影响，观察肛周局部肿胀溃疡程度并进行评分，ELISA检测血清IL-1

，TNF-

水平，HE染色观察直肠局部组织形态的影响。

结果：

生姜精油外敷大鼠涌泉穴治疗痔疮实验，与正常组比较，模型组血清IL-1

，TNF-

，IL-6，NO水平显著升高(

0.01)，直肠组织病理损伤严重；与模型组比较，各给药组可降低血清IL-6，NO，IL-1

，TNF-

水平(

0.05，

0.01)，减轻直肠组织损伤程度(

0.01)；生姜精油外涂肛周治疗痔疮实验，与正常组比较，模型组肛周肿胀、溃疡程度损伤严重(

0.01)，血清IL-1

，TNF-

水平显著升高(

0.01)，直肠组织病理损伤严重；与模型组比较，各给药组可改善肛周的病理状况(

0.01)，降低血清IL-1

，TNF-

水平(

0.01)，减轻直肠组织损伤程度(

0.01)。

结论：

生姜外用可有效抑制肛周肿胀和溃疡程度，对大、小鼠痔疮模型有很好的治疗作用。

Abstract

Objective:

To explore the effect of Zingiberis Rhizoma Recens(ZRR) on hemorrhoids in mice and rats.

Method:

Sixty SPF-grade SD rats were divided into blank group

model group

Ma Yinglong Shexiang hemorrhoid ointment group (7.5 g·kg

-1

)

and large and small-dose ZRR paste groups (10

5 g·kg

-1

). ZRR paste was applied in Yongquan acupoint to observe the effect of 0.05 mL injection with 75% acetic acid solution on hemorrhoids induced by subcutaneous anus in rats

the levels of interleukin-1

(IL-1

)

interleukin-6 (IL-6)

tumor necrosis factor-

(TNF-

)

nitric oxide (NO) in the serum were detected by enzyme linked immunosorbent assay(ELISA)

and the rectal histopathology was detected by hematoxylin-eosin(HE). Sixty SPF-grade KM mice were divided into blank group

model group

MA Ying-long Shexiang hemorrhoid ointment group (7.5 g·kg

-1

)

and large and small-dose ginger essential oil groups (0.06

0.03 mL

three times a day). ZRR paste was applied in crissum to observe the effect of injection of 20% acetic acid solution 0.05 mL (maintaining for 1 min) on hemorrhoids in mice induced by anus. The degree of local swelling ulcer around the anus and score was observed

levels of IL-1

TNF-

in the serum were determined by ELISA

and the rectal histopathology was detected by HE staining.

Result:

In the experimental study on treating hemorrhoids with ZRR paste applied on Yongquan point of rats

compared with normal group

serum levels of IL-1

IL-6

TNF-

NO in model group were significantly higher (

0.01). The pathological injury of rectum was serious. Compared with model group

serum levels of IL-1

IL-6

TNF-

NO were decreased in each administration group (

0.05

0.01)

and the degree of rectal tissue injury was relieved (

0.01). In the experiment of external application of ZRR essential oil around the anus for treating hemorrhoids

compared with normal group

perianal swelling and severe ulcer injury were observed in the model group (

0.01). The serum levels of IL-1

TNF-

in model group were significantly higher (

0.01)

and the pathological injury of rectum was serious. Compared with model group

each administration group showed the alleviation in perianal pathological conditions (

0.01)

the reduction in serum IL-1

TNF-

levels (

0.01)

and the relief in the extent of rectal tissue injury (

0.01).

Conclusion:

External application of ZRR can effectively inhibit perianal swelling and ulcer degree

with a good therapeutic effect on hemorrhoids model in rats and mice.

关键词

Keywords

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