Effect of Intestinal Flora in Rats on Metabolism and Transformation of Effective Components in Polygonum orientale Inflorescences

Shu-ting YANG; Si-ying CHEN; Hui-yuan SUN; Hao CHEN; Yong HUANG; Yue-ting LI; Yong-jun LI; Lin ZHENG; Yong-lin WANG

doi:10.13422/j.cnki.syfjx.20190447

您当前的位置：

首页 >

文章列表页 >

Effect of Intestinal Flora in Rats on Metabolism and Transformation of Effective Components in Polygonum orientale Inflorescences

Drug Metabolism | 更新时间：2021-02-09

- Effect of Intestinal Flora in Rats on Metabolism and Transformation of Effective Components in Polygonum orientale Inflorescences
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 142-149(2019)
- 作者机构：
  
  贵州医科大学　贵州省药物制剂重点实验室，省部共建药用植物功效与利用国家重点实验室，民族药与中药开发应用教育部工程研究中心，药学院，贵阳　 550004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190447
  CLC： R22; R969.1; O657; R378.2; R28; C37
- Received：15 September 2018，
  
  Published Online：02 November 2018，
  
  Published：20 July 2019
- 稿件说明：
移动端阅览
Shu-ting YANG, Si-ying CHEN, Hui-yuan SUN, et al. Effect of Intestinal Flora in Rats on Metabolism and Transformation of Effective Components in Polygonum orientale Inflorescences[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(14): 142-149.
DOI：

Shu-ting YANG, Si-ying CHEN, Hui-yuan SUN, et al. Effect of Intestinal Flora in Rats on Metabolism and Transformation of Effective Components in Polygonum orientale Inflorescences[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(14): 142-149. DOI： 10.13422/j.cnki.syfjx.20190447.

摘要

目的：

考察荭草花有效组分在大鼠肠道菌群中的代谢特征。

方法：

采用超高效液相色谱串联电喷雾四级杆飞行时间质谱联用技术(UPLC-ESI-Q-TOF-MS/MS)检测荭草花有效组分在大鼠体外肠道菌群中的孵育样品，流动相0.1%甲酸水溶液-0.1%甲酸乙腈溶液梯度洗脱，流速0.3 mL·min

－1

，电喷雾离子源(ESI)，正、负离子模式扫描，使用Metabolite Tools™和质量亏损过滤(MDF)等代谢产物分析技术，并结合化合物精确相对分子质量、碎片离子信息及文献数据，分析荭草花有效组分在大鼠体外肠道菌群中的代谢产物，推测其可能的生物转化途径。

结果：

荭草花有效组分经离体大鼠肠道菌群孵育后共检出18个代谢产物色谱峰，其生物转化途径主要是以还原、氧化、水解为主的Ⅰ相反应，甲基化为主的Ⅱ相反应。

结论：

荭草花有效组分能够在大鼠肠道菌群作用下转化为多种代谢产物，提示荭草花用药时，应考虑代谢产物是否为其生物活性成分。

Abstract

Objective:

To observe the metabolic characteristics of effective components from

Polygonum orientale

inflorescences in intestinal flora of rats.

Method:

The incubating samples of effective components from

orientale

inflorescences in rat intestinal flora

in vitro

were detected by UPLC-ESI-Q-TOF-MS/MS

the mobile phase was consisted of 0.1%formic acid solution-0.1%formic acid acetonitrile solution and eluted in gradient mode at a flow rate of 0.3 mL·min

-1

.The mass spectral analysis was detected with electrospray ionization under positive ion mode and negative ion mode.The metabolites and possible biotransformation pathways of effective components form

orientale

inflorescences in rat intestinal flora

in vitro

was analyzed by Metabolite Tools™

mass defect filtration(MDF) and other metabolite analysis techniques and combined with the accurate relative molecular weight of the compounds

the fragment ion information and the literature data.

Result:

Eighteen metabolites were detected after incubation of effective components from

orientale

inflorescences in rat intestinal flora.The main biotransformation pathways were reduction

oxidation

hydrolysis in Ⅰ phase reaction and methylation in Ⅱ phase reaction.

Conclusion:

The effective components of

orientale

inflorescences can be transformed into a variety of metabolites under the action of intestinal flora in rats.It is suggested that whether the metabolites are bioactive components should be considered when

orientale

inflorescences is used as medicine.

关键词

Keywords

references

向文英，梅朝叶，孙慧园，等．基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质 [J]．中国实验方剂学杂志， 2017 ， 23 ( 12 )： 90 - 95 ．

董莉，陈鹏程，唐丽，等．离体外翻肠囊法研究荭草花提取物中9个成分的肠吸收特征 [J]．中国医药工业杂志， 2014 ， 45 ( 9 )： 858 - 862 ．

李勇军，何迅，刘志宝，等．荭草花水溶性化学成分的研究 [J]．时珍国医国药， 2010 ， 21 ( 1 )： 14 - 15 ．

李勇军，何迅，刘志宝，等．荭草花化学成分的研究 [J]．中国中药杂志， 2009 ， 34 ( 20 )： 2613 - 2615 ．

张宇瑶，张慧，佟苗苗，等．响应面法优化荭草花提取工艺研究 [J]．中药材， 2014 ， 37 ( 2 )： 324 - 327 ．

黄勇，郑林，王爱民，等．荭草不同药用部位抗心肌缺血作用及化学成分比较研究 [J]．时珍国医国药， 2010 ， 21 ( 10 )： 2520 - 2522 ．

佟苗苗，张宇瑶，初正云，等．荭草花提取物体外抗肿瘤活性研究 [J]．中国民族民间医药， 2012 ， 22 ( 24 )： 7 - 8 ．

郑林，杨武，向文英，等． UPLC-MS/MS同时测定静脉注射荭草花提取物大鼠血浆中3种成分及其药代动力学研究 [J]．中药材， 2016 ， 39 ( 7 )： 1574 - 1577 ．

黄勇，胡杰，陆苑，等． UPLC-MS法测定大鼠血浆中荭草花的3个指标成分及其药动学研究 [J]．中国药理学通报， 2015 ， 31 ( 9 )： 1314 - 1318 ．

马祥雪，王凤云，符竣杰，等．基于肠道菌群的中医健脾方剂作用机制的研究现状与思考 [J]．中国实验方剂学杂志， 2017 ， 23 ( 5 )： 210 - 215 ．

林文振，李坤平，曾玉冰，等． UPLC-ESI-Q-TOF-MS/MS分析异鼠李素-3-O-β-D-芸香糖苷大鼠肠道菌群代谢产物的研究 [J]．中国实验方剂学杂志， 2012 ， 18 ( 20 )： 140 - 143 ．

覃小丽，孙慧园，杨武，等． UPLC-ESI-Q-TOF-MS/MS分析槲皮苷在大鼠肠道菌群中的代谢 [J]．中国中药杂志， 2017 ， 42 ( 2 )： 357 - 362 ．

刘亚丽，宋永贵，魏韶锋，等． UPLC-ESI-Q-TOF-MS/MS分析白头翁皂苷B3在大鼠肠道菌群中的代谢产物 [J]．中国实验方剂学杂志， 2015 ， 21 ( 23 )： 72 - 76 ．

CUI Q , PAN Y , XU X , et al . The metabolic profile of acteoside produced by human or rat intestinal bacteria or intestinal enzyme in vitro employed UPLC-Q-TOF-MS [J]. Fitoterapia , 2016 , 109 : 67 - 74 .

李勇军，李翠兵，何迅，等．荭草化学成分研究Ⅱ [J]．中国中药杂志， 2011 ， 36 ( 4 )： 458 - 461 ．

罗媛，杨武，孙慧园，等．没食子酸和原儿茶酸在大鼠肠道菌群中的代谢研究 [J]．沈阳药科大学学报， 2017 ， 34 ( 1 )： 43 - 47 ．

唐丽．贵州苗药头花蓼有效组分的体内外代谢研究 [D]．贵阳：贵阳医学院， 2015 ．

肖娟，王莹，王新宏，等．中药化学成分肠道菌群代谢的研究进展 [J]．中国实验方剂学杂志， 2012 ， 18 ( 5 )： 247 - 251 ．

门薇，陈颖，李玉洁，等．肠道菌群对中药有效成分的生物转化研究进展 [J]．中国实验方剂学杂志， 2015 ， 21 ( 2 )： 229 - 234 ．

邓颖，郭志刚，曾兆麟，等．藏红花的药理研究进展 [J]．中国中药杂志， 2002 ， 34 ( 8 )： 8 - 11 ．

刘亭，吴琼，刘香香，等．荭草花提取物对H9c2心肌细胞缺氧复氧损伤的保护作用 [J]．中药材， 2018 ， 41 ( 1 )： 184 - 188 ．

郑林，陈慧，王爱民，等．超高效液相色谱测定大鼠口服荭草花后入血成分 [J]．中国医院药学杂志， 2012 ， 32 ( 2 )： 91 - 93 ．

谢玉敏，梅朝叶，陈浩，等．水荭花提取物的药动学特性及绝对生物利用度研究 [J]．中国抗生素杂志， 2017 ， 42 ( 7 )： 611 - 615 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis of in Vivo and in Vitro Metabolites of Coptisine in Rats by HPLC-MS/MS

Mechanism of Paeonol in Alleviating Alcohol-induced Liver Injury in Mice Through Regulating SCFAs-GPR43/MAPK Signaling Pathway Mediated by Intestinal Flora

Effect of Huangling Jidu Xizhuo Granules on NLRP3， Intestinal Flora and Short-term Prognosis in Patients with Gout

Mechanism and Combination Therapy of Berberine in Treatment of Nonalcoholic Fatty liver Disease：A Review

Mechanisms of Intestinal Microecology in Hyperuricemia and Traditional Chinese Medicine Intervention：A Review

Related Author

Hong-ying CHEN

Yun-fang HUANG

Qi-hua LIU

Wei ZHENG

Zhi-hui LI

Jian-hua CHEN

Jing QI

Ting ZHANG

Related Institution

School of Chinese Materia Medica, Beijing University of Chinese Medicine

Guang'anmen Hospital, China Academy of Chinese Medical Sciences

College of Pharmacy，Anhui University of Chinese Medicine

School of Integrated Chinese and Western Medicine，Anhui University of Chinese Medicine

Geriatric Care and Health Laboratory，Anhui University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰