Effect of Houpu Sanwu Tang on Small Intestine Interstitial Cells of Cajal and iNOS in Postoperative Ileus Rats

Xiu-rong YANG; Xiao-hong ZHAO; Hui CHEN; Su-ning CHEN; Fei YU

doi:10.13422/j.cnki.syfjx.20190507

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Effect of Houpu Sanwu Tang on Small Intestine Interstitial Cells of Cajal and iNOS in Postoperative Ileus Rats

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Houpu Sanwu Tang on Small Intestine Interstitial Cells of Cajal and iNOS in Postoperative Ileus Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 5, Pages: 57-62(2019)
- 作者机构：
  
  中国医科大学　附属盛京医院，沈阳　 110004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190507
  CLC： R2-0;R22;R285.5;R289
- Received：05 August 2018，
  
  Published Online：26 November 2018，
  
  Published：05 March 2019
- 稿件说明：
移动端阅览
Xiu-rong YANG, Xiao-hong ZHAO, Hui CHEN, et al. Effect of Houpu Sanwu Tang on Small Intestine Interstitial Cells of Cajal and iNOS in Postoperative Ileus Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 57-62.
DOI：

Xiu-rong YANG, Xiao-hong ZHAO, Hui CHEN, et al. Effect of Houpu Sanwu Tang on Small Intestine Interstitial Cells of Cajal and iNOS in Postoperative Ileus Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 57-62. DOI： 10.13422/j.cnki.syfjx.20190507.

摘要

目的：

以Cajal间质细胞(ICC)及诱导型一氧化氮合酶(iNOS)表达为切入点，观察厚朴三物汤对术后肠梗阻大鼠小肠炎症反应与神经反馈的影响，并探讨其对胃肠道运动作用的相关机制。

方法：

健康成年雄性SD大鼠87只，随机分为假手术组、模型组、厚朴三物汤低、中、高剂量组。采用手术方法造模，造模后分别给予生理盐水或不同剂量的厚朴三物汤灌胃，低、中、高剂量组给药量分别为2.25，4.5，9g·kg

－1

。给药后观察各组大鼠术后不同时间的小肠推进率及小肠形态学变化，免疫组织化学法检测ICC细胞及iNOS的表达情况。

结果：

与假手术组比较，造模后大鼠小肠推进率明显下降(

0.01)，造模后可见小肠肠管明显扩张、胀气，苏木素-伊红(HE)染色可见小肠绒毛破裂，结构不完整，黏膜下可见血管充血水肿。与盐水组比较，厚朴三物汤中、高剂量组大鼠24，48h小肠推进率显著提高(

0.01)，而且厚朴三物汤治疗后3组在48h内大鼠小肠推进率均随时间延长而增加(

0.01)。HE染色可见大鼠肠黏膜绒毛脱落及组织水肿程度较盐水组明显减轻。免疫组化显示造模后大鼠小肠Cajal间质细胞表达下降，iNOS阳性表达显著升高；而厚朴三物汤治疗后3组Cajal间质细胞在24，48h均显著升高，中、低剂量组12，24h iNOS阳性表达明显降低(

0.05，

0.01)。

结论：

厚朴三物汤能够显著提高术后肠梗阻模型大鼠的小肠推进率，促进大鼠术后胃肠动力的恢复，其作用机制与抑制iNOS的产生，减轻炎症反应，同时增加ICC数量，保证其正常功能，从而改善肠动力障碍有关。

Abstract

Objective:

To evaluate the effect of Houpu Sanwu Tang on the postoperative ileus (POI)

and observe its underlying mechanisms of action on interstitial cells of cajal (ICC) and inducible nitric oxide synthase(iNOS) regulation of POI.

Method:

Totally 87 healthy adult male SD rats were randomly divided into sham operation group

saline control group and Houpu Sanwu Tang group at low

medium and high doses. Houpu Sanwu Tang low

middle and high dose groups received orally Houpu Sanwu Tang(2.25

4.5

9g·kg

-1

); Sham operation group (Sham operation) and saline control group (Saline control) received orally normal saline. Surgical procedure was used to induce the postoperative ileus. Changes in intestinal propulsion rate

intestinal mucosal injury and small intestine expression of c-kit and iNOS among these groups were detected.

Result:

Intestinal propulsion rate was significantly higher in Houpo Sanwu Tang group than that in Saline control group (

0.01). Rats in Houpo Sanwu Tang group showed reduced intestinal mucosal injury compared with those in Saline control group. C-kit in Houpo Sanwu Tang groups was significantly increased (

0.01) at 24 h and 48 h

and iNOS in low-dose group and middle-dose group was significantly reduced (

0.05

0.01) at 12 h and 24 h compared with those in the Saline control group.

Conclusion:

Houpu Sanwu Tang can improve the intestinal propulsion rate and the recovery in POI rats. The mechanisms are related to the inhibition of the generation of iNOS

the alleviation of inflammatory response

and increase of the number of ICC

so as to ensure its normal function

and improve the intestinal dynamic disorder.

关键词

Keywords

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