Objective:

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To study the chemical constituents of Bletillae Formosanae Rhizoma and the distribution characteristics of active components in the fingerprint by establishing its high performance liquid chromatography (HPLC) fingerprints.

Method:

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HPLC was used to establish the fingerprint of Bletillae Formosanae Rhizoma. Four reference substances

i.e. militarine

coelonin

4-methoxy-9

10-dihydrophenanthrene-1

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7-triol and batatasin Ⅲ were used to identify chromatographic peaks. The fingerprints of 17 batches of Bletillae Formosanae Rhizoma fingerprints were analyzed and compared by "Computer-aided-similarity evaluation soft" and stoichiometry

and then compared with the fingerprint of Bletillae Rhizoma.

Result:

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The established HPLC fingerprint method of Bletillae Formosanae Rhizoma showed good repeatability and stability. 20 common peaks were marked

four of which were identified by reference substances; militarine was No.8 common peak

and others corresponded to No. 10

No. 14 and No. 18 common peaks. Results showed that the similarities of samples except S4 were higher than 0.85

but the relative peak area of common peaks was quite different. Within the cluster distance 10

the samples are clustered into 5 categories

reflecting certain origin correlation. Principal component analysis (PCA) showed that the difference in samples was mainly caused by the common peaks located after No. 9 peak

where chemical constituents such as bibenzyl and dihydrophenanthrene were distributed. Bletillae Formosanae Rhizoma and Bletillae Rhizoma showed similar chemical constituents.

Conclusion:

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The method provided a theoretical basis for the further clinical application and quality control of Bletillae Formosanae Rhizoma

as a substitute for Bletillae Rhizoma.