Objective:

2

To study the correlation between the fingerprint of different polar parts of Huanbei Zhike recipe and its antitussive effect

identify the chromatographic peaks greatly contributing to its pharmacological effect

and ascribe the chromatographic peaks.

Method:

2

Gradient elution method was used to establish the chromatographic peaks of different polar parts of Huanbei Zhike recipe. The common peaks and corresponding peak areas of each part were obtained by referring to the water extract chromatogram and after being processed by chromatographic workstation. The cough frequency

the latent period of cough and the contents of interleukin (IL)-4

interferon-

γ

(IFN-

γ

)

immunoglobulin (Ig)E and IL-5 in lung lavage fluid of mice were used as the detection indexes to investigate the antitussive effect of different polar parts of Huanbei Zhike recipe. The correlation analysis among cough frequency

cough latent period and common peak area was carried out

and the peaks greatly contributing to the effect of medicine were pointed out

which were assigned according to the fingerprint of single drug.

Result:

2

Bivariate correlation analysis showed that seven chromatographic peaks

i. e. No.12

13

29

32

33

35 and 39

contributed significantly to the antitussive effect. The higher the content of the compounds represented by these peaks

the stronger the antitussive effect. There was a significant correlation between No. 32 peak and the latent period of cough

while No. 13 and No. 32 peaks were significantly correlated with cough frequency. These chromatographic peaks were assigned and found to be present in

Scutellaria baicalensis

Crepis Herba

Aurantii Fructus

Glycyrrhiza uralensis

etc.

Conclusion:

2

The chromatographic peaks of No.13 and No.32 peaks contribute greatly to the antitussive effect of Huanbei Zhike recipe

while

Crepis turczaniowii

S

.

baicalensis

and Aurantii Fructus are of significant significance for the whole recipe.