Effect of Dry-cold Environment on Metabolic Components in Urine and Plasma of Rats

Aheerzhama-ni MAIMAITI; Mirea-yi YALIKUN; Tuer-hong TAILAITI; Dina-la QIAREFUHAN; Batur MAMTIMIN

doi:10.13422/j.cnki.syfjx.201906

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Effect of Dry-cold Environment on Metabolic Components in Urine and Plasma of Rats

Drug Metabolism | 更新时间：2021-02-09

- Effect of Dry-cold Environment on Metabolic Components in Urine and Plasma of Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 11, Pages: 115-120(2019)
- 作者机构：
  
  新疆医科大学　维吾尔医学院，药学院，中心实验室，乌鲁木齐　 830011
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.201906
  CLC： R22; R24; R28; C37; R969.1
- Received：21 September 2018，
  
  Published Online：05 December 2018，
  
  Published：05 June 2019
- 稿件说明：
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Aheerzhama-ni MAIMAITI, Mirea-yi YALIKUN, Tuer-hong TAILAITI, et al. Effect of Dry-cold Environment on Metabolic Components in Urine and Plasma of Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 115-120.
DOI：

Aheerzhama-ni MAIMAITI, Mirea-yi YALIKUN, Tuer-hong TAILAITI, et al. Effect of Dry-cold Environment on Metabolic Components in Urine and Plasma of Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 115-120. DOI： 10.13422/j.cnki.syfjx.201906.

摘要

目的：

通过核磁共振氢谱(

H-NMR)分析方法探讨干寒环境及饮食对大鼠尿液和血浆代谢物的影响，寻找可能的生物标记物及其相关代谢途径。

方法：

利用干寒环境及饮食作为应激源干预大鼠3周，采集其尿液和血浆样本，检测

H-NMR，结合多变量数据分析方法筛选与鉴定相关代谢标志物，并分析代谢通路。

结果：

干寒属性环境及饮食导致尿液23种代谢物，血浆中15种代谢物发生了显著变化；这些代谢物与多种代谢通路的变化密切相关，包括三羧酸循环，丙酮酸代谢，糖酵解或糖异生，缬氨酸、亮氨酸和异亮氨酸生物合成，甘氨酸、丝氨酸和苏氨酸代谢，组氨酸代谢，乙醛酸和二羧酸代谢，丙氨酸、天冬氨酸和谷氨酸代谢通路。

结论：

干寒环境及饮食条件可导致体内多种代谢通路的变化，基于

H-NMR的代谢组学研究方法具有解释机体病理生理动态性，整体性变化的优势，对于探索非确定性疾病因素发病机制有着重要的意义。

Abstract

Objective:

Nuclear magnetic resonance hydrogen spectrum(

H-NMR) was adopted to investigate the effect of dry-cold environment and diet on urine and plasma metabolites of rats

in order to find possible biomarkers and their related metabolic pathways.

Method:

The dry-cold environment and diet were used as a stressors to intervene rats for 3 weeks

urine and plasma samples were collected.

H-NMR combined with multivariate data analysis were used to identify relevant metabolic markers and analyze their metabolic pathways.

Result:

The dry-cold environment and diet resulted in significant changes of 23 metabolites in urine and 15 metabolites in plasma; these metabolites were closely related to changes of various metabolic pathways

namely the tricarboxylic acid(TCA) cycle

pyruvate metabolism

glycolysis or gluconeogenesis

valine

leucine and isoleucine biosynthesis

glycine

serine and threonine metabolism

histidine metabolism; glyoxylic acid and dicarboxylic acid metabolism

alanine

aspartic acid and glutamate metabolism.

Conclusion:

Dry-cold environment and diet conditions can lead to the changes of various metabolic pathways in the body.The metabolomics based on

H-NMR has the advantage of explaining the pathophysiological dynamics and overall changes of the body

which is of great significance for exploring the pathogenesis of non-deterministic disease factors.

关键词

Keywords

references

谢宁．中医学基础 [M]． 9 版．北京：中国中医药出版社， 2012 ： 5，106 ．

王洋，蔡志钢，张永煜．湿热环境对CCl4诱导大鼠肝炎模型的影响及代谢组学研究 [J]．世界科学技术—中医药现代化， 2018 ， 20 ( 1 )： 101 - 108 ．

Lean M E , Leslie W S , Bames A C , et al . Primary care-led weight management for remission of type 2 diabetes(DiRECT): an open-label, cluster-randomised trial [J]. Lancet , 2017 , 391 ( 10120 ): 541 - 551 .

吴家睿． “健康”饮食需要关注的因素 [J]．科学(上海) ， 2018 ， 70 ( 4 )： 23 - 26 ．

王静，陈悦，袁子民，等．基于尿液代谢组学分析肉豆蔻麸煨炮制前后对大鼠长期毒性的作用差异 [J]．中国实验方剂学杂志， 2018 ， 24 ( 4 )： 8 - 13 ．

郭慧，崔扬，王秋红，等．基于代谢组学技术的中药复方研究近况 [J]．中国实验方剂学杂志， 2017 ， 23 ( 1 )： 213 - 219 ．

《中医堂》编委会．中华中草药图谱全典 [M]．哈尔滨：黑龙江科学技术出版社， 2015 ： 14 ．

李时珍．本草纲目彩色图典 [M]．易磊，牛林敬，译．北京：人民军医出版社， 2013 ： 98 ．

XIA J , Sinelnikov I V , HAN B , et al . MetaboAnalyst 3.0——making metabolomics more meaningful [J]. Nucleic Acids Res , 2015 , 43 ( W1 ): W251 - W257 .

SHAN L , LIAO F , JIN H , et al . Plasma metabonomic profiling of lumbar disc herniation and is traditional Chinese medicine subtypes in patients by using gas chromatography coupled with mass spectrometry [J]. Mol Biosyst , 2014 , 10 ( 11 ): 2965 - 2973 .

张铭．稳态应激——变化的稳态 [J]．生理科学进展， 2015 ， 46 ( 4 )： 269 - 272 ．

严进，路长林，刘振全．现代应激理论概述 [M]．北京：科学出版社， 2008 ， 3 - 26 ．

Carter J R , Goldstein D S . Sympathoneural and adrenomedullary responses to mental stress [J]. Compr Physiol , 2015 , 5 ( 1 ): 119 - 146 .

郭峰，谢榆，袁滨．基于中医体质学说的运动疗法在干预高尿酸血症中的临床观察 [J]．贵州医药， 2018 ， 42 ( 1 )： 65 - 67 ．

Amelio I , Cutruzzolá F , Antonov A , et al . Serine and glycine metabolism in cancer [J]. Trends Biochem Sci , 2014 , 39 ( 4 ): 191 - 198 .

沈淑洁，水素芳，肖炳坤，等．基于1H-NMR及LC-MS技术研究金铃子散对炎症大鼠模型调节机制的影响 [J]．中国中药杂志， 2017 ， 42 ( 2 )： 363 - 369 ．

So P W , Fuller B J . Enhanced energy metabolism during cold hypoxic organ preservation: studies on rat liver after pyruvate supplementation [J]. Cryobiology , 2003 , 46 ( 3 ): 295 - 300 .

Stanko R T , Tietze D L , Arch J E . Body composition, energy utilization, and nitrogen metabolism with a severely restricted food supplemented with dihydroxyacetone and pyruvate [J]. Am J Clin Nutr , 1992 , 55 ( 4 ): 771 - 776 .

牛倩芸，刘月涛，李震宇，等．基于核磁代谢组学技术的阿霉素肝毒性研究 [J]．药学学报， 2015 ， 50 ( 6 )： 708 - 713 ．

Patti M E , Corvera S . The role of mitochondria in the pathogenesis of type 2 diabetes [J]. Endocr Rev , 2010 , 31 ( 3 ): 364 - 395 .

Holmes E , Nicholls A W , Lindon J C , et al . Chemometric models for toxicity classification based on NMR spectra of biofluids [J]. Chem Res Toxicol , 2000 , 13 ( 6 ): 471 - 478 .

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