Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β<sub>1</sub>

Jing PAN; Yue-guang DU; Yi GUO

doi:10.13422/j.cnki.syfjx.20190636

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Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β₁

Pharmacology | 更新时间：2021-02-09

- Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β₁
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 6, Pages: 89-94(2019)
- 作者机构：
  
  浙江中医药大学　基础医学院，杭州　 310053
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190636
  CLC： R285.5;R973+.6;R973+.1;S941.42+7
- Received：20 October 2018，
  
  Published Online：12 July 2018，
  
  Published：20 March 2019
- 稿件说明：
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Jing PAN, Yue-guang DU, Yi GUO. Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β₁[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 89-94.
DOI：

Jing PAN, Yue-guang DU, Yi GUO. Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β₁[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 89-94. DOI： 10.13422/j.cnki.syfjx.20190636.

摘要

目的：

研究三七皂苷(PNS)对转化生长因子-

(TGF-

)诱导的大鼠肾小管细胞上皮-间充质转化(EMT)的干预作用，并从沉默信息调节因子2相关酶1(SIRT1)/TGF-

/Smad通路分析其可能机制。

方法：

在DMEM培养基中，用10%胎牛血清培养大鼠肾小管上皮细胞(NRK-52E)，分为正常组，TGF-

组(5 μg·L

－1

)，白藜芦醇组(50 mg·L

－1

)，SIRT1阻断剂EX527组(10 μmol·L

－1

)，PNS组(100 mg·L

－1

)，EX527＋PNS组(EX527 10 μmol·L

－1

，PNS 100 mg·L

－1

)，48 h后收集细胞；采用实时荧光定量聚合酶链式反应(Real-time PCR)检测SIRT1，

-平滑肌肌动蛋白(

-SMA)，E-钙黏附蛋白(E-cadherin)，TGF-

，Smad3，Smad4 mRNA表达；蛋白免疫印迹法(Western blot)检测SIRT1，

-SMA，E-cadherin，TGF-

蛋白表达。

结果：

与正常组比较，TGF-

组

-SMA mRNA及蛋白表达明显增多(

0.05)，E-cadherin表达显著减少(

0.01)；与TGF-

组比较，PNS，白藜芦醇组，E-cadherin mRNA及蛋白表达均显著增加(

0.01)，

-SMA表达显著减少(

0.01)，EMT发生被抑制，同时，SIRT1表达显著增加(

0.01)，TGF-

，Smad3，Smad4表达显著降低(

0.01)。在SIRT1阻断剂EX527的干预下，PNS则不能发挥明显抑制作用。

结论：

PNS可以阻止TGF-

诱导的肾小管上皮细胞EMT的发生，其机制可能是通过激活SIRT1进而抑制TGF-

/Smad通路实现的。

Abstract

Objective:

To investigate the intervention effect of panax notoginseng saponins (PNS) on epithelial-mesenchymal transition (EMT) of rat renal tubular epithelial cells (NRK-52E) induced by transforming growth factor-

(TGF-

)

and analyze the mechanism based on the silent information regulation 2 homolog 1(SIRT1)/TGF-

/Smad signaling pathway.

Method:

NRK-52E were cultured in DMEM medium with 10%fetal bovine serum

and divided into normal control group

TGF-

group (5 μg·L

-1

)

resveratrol (RSV) group (50 mg·L

-1

)

EX527 group (10 μmol·L

-1

)

Panax notoginseng saponins (PNS) group (100 mg·L

-1

)

and EX527+ PNS group (10 μmol·L

-1

+ 100 mg·L

-1

). Then cells were collected after drug intervention for 48 h. The expressions of

-SMA

E-cadherin

SIRT1

TGF-

Smad3

Smad4 mRNA in each group were detected by Real-time PCR. The protein expressions of

-SMA

E-cadherin

SIRT1 and TGF-

were detected by Western blot.

Result:

Compared with normal group

mRNA and protein expressions of

-SMA increased obviously(

0.05)

but E-cadherin decreased significantly(

0.01)in TGF-

group. Compared with TGF-

group

mRNA and protein expressions of

-SMA decreased significantly(

0.01)

while E-cadherin increased(

0.01)in resveratrol and PNS groups

and EMT was inhibited. Meanwhile

mRNA and protein expressions of SIRT1 increased significantly(

0.01)

while mRNA expressions of TGF-

Smad3

and Smad4 decreased(

0.01). Under the intervention of SIRT1 blocker EX527

PNS could not play a significant inhibitory effect on the cells.

Conclusion:

PNS can prevent the occurrence of EMT of renal tubular epithelial cells induced by TGF-

and the mechanism may be related to active SIRT1 to inhibit TGF-

/Smad pathway.

关键词

Keywords

references

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Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β1

DOI：10.13422/j.cnki.syfjx.20190636

摘要

Abstract

关键词

Keywords

references

Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β₁