Effect of Gandou Decoction on Immune Function of Spleen in TX Mice of Wilson's Disease Model

Sheng ZHOU; Yi-jie WANG; Wen-bin HU; Xu-en YU; Nan CHENG; Jun-xia WU

doi:10.13422/j.cnki.syfjx.20190703

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Effect of Gandou Decoction on Immune Function of Spleen in TX Mice of Wilson's Disease Model

Experimental Study on Treatment of Wilson's Disease with Gandou Decoction | 更新时间：2021-02-09

- Effect of Gandou Decoction on Immune Function of Spleen in TX Mice of Wilson's Disease Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 68-74(2019)
- 作者机构：
  
  1.安徽中医药大学　研究生院，合肥　 230038；
  2.安徽中医药大学　神经病学研究所　附属医院，合肥　 230061
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190703
  CLC： R2-0;R22;R285.5;R289
- Received：09 October 2018，
  
  Published Online：19 December 2018，
  
  Published：05 April 2019
- 稿件说明：
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Sheng ZHOU, Yi-jie WANG, Wen-bin HU, et al. Effect of Gandou Decoction on Immune Function of Spleen in TX Mice of Wilson's Disease Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 68-74.
DOI：

Sheng ZHOU, Yi-jie WANG, Wen-bin HU, et al. Effect of Gandou Decoction on Immune Function of Spleen in TX Mice of Wilson's Disease Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 68-74. DOI： 10.13422/j.cnki.syfjx.20190703.

摘要

目的：

探讨肝豆汤对Wilson病模型TX小鼠脾脏免疫功能的调节作用，并探讨相关的作用机制。

方法：

将小鼠分为正常组、模型组、四硫钼酸铵组、肝豆汤低、中、高剂量组，每组20只。正常组选取DL小鼠常规饲养30 d；模型组和肝豆汤低、中、高剂量组选取TX小鼠分别予生理盐水、肝豆汤(22，44，66 g·kg

－1

)各2 mL·kg

－1

·d

－1

灌胃，四硫钼酸铵组予四硫钼酸铵2 mg·kg

－1

·d

－1

灌胃，每日2次，连续30 d。上述4组采用电感耦合等离子体-质谱法(ICP-MS)测脾组织微量元素，流式细胞术检测小鼠脾组织T淋巴细胞亚群CD4

＋

，CD8

＋

，CD4

＋

/CD8

＋

；蛋白免疫印迹法(Western blot)检测白细胞介素-2(interleukin-2，IL-2)，白细胞介素-8(interleukin-8，IL-8)，白细胞介素-17(interleukin-17，IL-17)，白细胞介素-18(interleukin-18，IL-18)，

-干扰素(interferon-

，IFN-

)，肿瘤坏死因子-

(tumor necrosis factor-

，TNF-

)的蛋白表达量。

结果：

ICP-MS结果显示：与模型组比较，肝豆汤可以降低TX小鼠脾组织铜含量(

0.01)，流式细胞术检测显示，与正常组比较，模型组脾组织内CD4

＋

，CD8

＋

增高(

0.01)，CD4

＋

/CD8

＋

降低(

0.01)，与模型组比较，肝豆汤中、高剂量组脾组织内CD4

＋

，CD8

＋

降低(

0.05)，CD4

＋

/CD8

＋

增高，但差异无统计学意义，肝豆汤低剂量组与模型组比较CD4

＋

，CD8

＋

降低，CD4

＋

/CD8

＋

增高，但差异均无统计学意义；Western blot检测显示，与正常组比较，模型组脾组织内IL-2，IL-8，IL-17，IL-18，TNF-

，IFN-

的蛋白表达量增高(

0.01)，与模型组比较，肝豆汤中、高剂量组及四硫钼酸铵组IL-2，IL-8，IL-17，IL-18，TNF-

，IFN-

蛋白表达量均降低(

0.05，

0.01)；与模型组比较，低剂量肝豆汤组IL-2，IL-8，IL-17，TNF-

相对表达量降低(

0.05)。

结论：

TX小鼠脾脏存在免疫功能紊乱，且以负向免疫调节为主。肝豆汤对TX小鼠脾脏免疫功能紊乱具有一定的改善作用。

Abstract

Objective:

To discuss the effect of Gandou decoction (GDD) on the immune index of spleen in TX mice of Wilson's disease model.

Method:

The mice were divided into normal group

model group and GDD or tetrathiomolybdate(TM)treatment group

with 20 mice in each group. Each group was fed in various ways for 30 successive days. Normal group: 10 normal DL mice were randomly selected and feed normally. Model group: 20 TX mice were randomly selected and feed with 2 mL·kg

-1

·d

-1

saline by gavage twice per day. GDD or TM treatment group: 80 TX mice were randomly selected and feed with 2 mL·kg

-1

·d

-1

Gandou decoction 22

66 g·kg

-1

or tetrathiomolybdate by gavage twice per day. ICP-MS was used to compare the expressions of trace elements inside the mice's spleens

flow cytometry was applied to detect the mice T lymphocyte subsets of splenic tissue CD4

CD8

CD4

/CD8

and Western blot was used to detect the ex

ressions of interferon-

(IFN-

)

tumor necrosis factor-

(TNF-

)

interleukin-2 (IL-2)

interleukin-8 (IL-8)

interleukin-17 (IL-17) and interleukin-18 (IL-18).

Result:

Flow ICP-MS results showed that GDD can reduce Cu of mice's spleen

flow cytometry results showed that CD4

and CD8

in model group were increased than those in normal group (

0.01)

and CD4

/CD8

was decreased (

0.01); compared with model group

CD4

and CD8

in middle and high-dose GDD groups were decreased (

0.01)

and CD4

/CD8

was increased. According to Western blot detection

compared with normal group

the expressions of IL-2

IL-8

IL-17

IL-18

TNF-

and IFN-

in the model group were increased (

0.01); compared with model group

the expressions of TNF-

IFN-

IL-2

IL-8

IL-17 and IL-18 in the GDD middle and high or TM group were decreased (

0.05

0.01). Compared with model group

the expressions of IL-2

IL-8

IL-17 and TNF-

in the GDD low were decreased (

0.05).

Conclusion:

Spleen of TX mice shows the cellular immunity hyperfunction

which is mainly dominated by the negative immunoloregulation. GDD has a certain effect in regulating cellular immunity hyperfunctional state of TX mice

but it's difficult to thoroughly change the negative immune regulation.

关键词

Keywords

references

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