Inhibitory Effect of Cinnamaldehyde on Proliferation, Migration and Tube Formation of VEGF-induced Endothelial Cells via JAK2/STAT3 Pathway

Ming-xia CHEN; Jian-xun LIU; Qu-xing WU; Pan WANG; Zeng-lin LIAN; Ye LIU; Zhi-gang LI; Jun-guo REN

doi:10.13422/j.cnki.syfjx.20190704

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Inhibitory Effect of Cinnamaldehyde on Proliferation, Migration and Tube Formation of VEGF-induced Endothelial Cells via JAK2/STAT3 Pathway

Pharmacology | 更新时间：2021-02-09

- Inhibitory Effect of Cinnamaldehyde on Proliferation, Migration and Tube Formation of VEGF-induced Endothelial Cells via JAK2/STAT3 Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 8, Pages: 28-33(2019)
- 作者机构：
  
  1.中国中医科学院　西苑医院　基础医学研究所，中药药理北京市重点实验室，北京　 100091；
  2.北京汉典制药有限公司　中药研究所，北京　 100103
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190704
  CLC： R2-0;R22;R285.5
- Received：30 August 2018，
  
  Published Online：18 December 2018，
  
  Published：20 April 2019
- 稿件说明：
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Ming-xia CHEN, Jian-xun LIU, Qu-xing WU, et al. Inhibitory Effect of Cinnamaldehyde on Proliferation, Migration and Tube Formation of VEGF-induced Endothelial Cells via JAK2/STAT3 Pathway[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 28-33.
DOI：

Ming-xia CHEN, Jian-xun LIU, Qu-xing WU, et al. Inhibitory Effect of Cinnamaldehyde on Proliferation, Migration and Tube Formation of VEGF-induced Endothelial Cells via JAK2/STAT3 Pathway[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 28-33. DOI： 10.13422/j.cnki.syfjx.20190704.

摘要

目的：

为探讨桂皮醛对糖尿病视网膜病变新生血管的作用及机制，观察了桂皮醛对血管内皮生长因子(VEGF)诱导EA.hy926细胞增殖、迁移、成管以及Janus激酶2/信号传导与转录激活因子3(JAK2/STAT3)通路的影响。

方法：

将EA.hy926细胞分成空白组、模型组(7 μg·L

-1

VEGF)，VEGF+桂皮醛(60

120

150 μmol·L

-1

)组，分别采用噻唑蓝(MTT)比色法和划痕实验检测桂皮醛对VEGF诱导EA.hy 926细胞增殖和迁移作用的影响；将EA.hy 926细胞分成空白组，模型组(7 μg·L

-1

VEGF)，VEGF+桂皮醛(90

150 μmol·L

-1

)组，采用管腔形成实验检测桂皮醛对VEGF诱导EA.hy 926细胞成管作用的影响；将EA.hy 926细胞分成空白组，模型组(7 μg·L

-1

VEGF)，VEGF+AG490(50 μmol·L

-1

)组，VEGF+桂皮醛(90 μmol·L

-1

)组，VEGF+桂皮醛(150 μmol·L

-1

)组，VEGF+桂皮醛(150 μmol·L

-1

)+AG490(50 μmol·L

-1

)组，采用蛋白免疫印迹法(Western blot)检测桂皮醛对VEGF诱导EA.hy 926细胞JAK2/STAT3通路的影响。

结果：

与空白组比较，模型组能够显著地促进EA.hy 926细胞增殖和迁移(

0.01)。与模型组比较，桂皮醛(60

120

150 μmol·L

-1

)组能显著抑制VEGF诱导EA.hy 926细胞的增殖和迁移(

0.01)。与空白组比较，VEGF对EA.hy 926细胞成管具有一定的促进作用，成管的节点数、交叉点数、网眼数和血管分支数均有增加，但无统计学差异。与模型组比较，桂皮醛(90

150 μmol·L

-1

)组对成管的节点数、交叉点数和网眼数均有明显抑制作用(

0.05，

0.01)。与空白组比较，模型组p-JAK2，p-STAT3，STAT3蛋白表达明显升高(

0.05，

0.01)。与模型组比较，桂皮醛(150 μmol·L

-1

)能够显著抑制VEGF引起的p-JAK2，p-STAT3，STAT3蛋白表达升高(

0.01)，桂皮醛(90 μmol·L

-1

)能够显著抑制VEGF引起的p-STAT3，STAT3蛋白表达升高(

0.05，

0.01)。

结论：

桂皮醛对VEGF诱导EA.hy 926细胞的增殖、迁移、成管具有明显的抑制作用，该作用与抑制JAK2/STAT3通路的激活有关。

Abstract

Objective:

To investigate the effect and mechanism of cinnamaldehyde on the angiogenesis of diabetic retinopathy

and the effect of cinnamaldehyde on vascular endothelial growth factor (VEGF) induced proliferation

migration

tube formation and Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway of EA.hy 926 cells were observed.

Method:

EA.hy 926 cells were divided into normal control group

model group (7 μg·L

-1

VEGF)

and VEGF+ cinnamaldehyde group (60

120

150 μmol·L

-1

). The methyl thiazolyl tetrazolium (MTT) assay and scratch test were used to observe the effect of cinnamaldehyde on the proliferation and migration of EA.hy 926 cells induced by VEGF. EA.hy 926 cells were divided into normal control group

model group (7 μg·L

-1

VEGF)

and VEGF+ cinnamaldehyde group (90

150 μmol·L

-1

). The tube formation experiment was used to observe the effect of cinnamaldehyde on the tube formation of EA.hy 926 cells induced by VEGF. EA.hy 926 cells were divided into normal control group

model group (7 μg·L

-1

VEGF)

VEGF+ AG490 group (50 μmol·L

-1

)

VEGF+ cinnamaldehyde group (90 μmol·L

-1

)

VEGF+ cinnamaldehyde group (150 μmol·L

-1

)

and VEGF+ cinnamaldehyde group (150 μmol·L

-1

)+ AG490 group (50 μmol·L

-1

). Western Blot method was used to explore the effect of cinnamaldehyde on the JAK2/STAT3 signaling pathway in EA.hy 926 cells induced by VEGF.

Result:

Compared with the control group

model group obviously promoted the proliferation and migration of EA.hy 926 cells(

0.01). Compared with the model group

cinnamaldehyde (60

120

150 μmol·L

-1

) significantly suppressed VEGF-induced proliferation and migration of EA.hy 926 cells (

0.01). Compared with the control group

VEGF group could promote the tube formation of EA.hy 926 cells. The number of nodes

junctions

meshes and vascular branches were increased

but with no statistical difference. Compared with the model group

cinnamaldehyde (90

150 μmol·L

-1

) showed an obvious inhibitory effect on the number of nodes

junctions and meshes of tubules (

0.05

0.01). Compared with the control group

the expressions of p-JAK2

p-STAT3

and STAT3 in the model group were significantly increased (

0.05

0.01). Compared with the model group

Cinnamaldehyde (150 μmol·L

-1

) significantly reduced the expressions of P-JAK2

P-STAT3

STAT3 proteins (

0.01). Cinnamaldehyde (90 μmol·L

-1

) obviously reduced the expressions of p-STAT3 and STAT3 proteins (

0.05

0.01).

Conclusion:

Cinnamaldehyde showed a significantly inhibitory effect on the proliferation

migration and tube formation of VEGF-induced EA.hy 926 cells

which was related to the inhibition of the activation of JAK2/STAT3 pathway.

关键词

Keywords

references

冯宇梁，李杰，刘巾男，等．视网膜脉络膜新生血管性疾病机制及治疗研究进展 [J]. 西部医学， 2016 , 28 ( 9 ): 1328 - 1333 .

宋剑涛，高健生，接传红，等．密蒙花方干预早期糖尿病视网膜病变初步疗效报告 [J]. 中国中医眼科杂志， 2010 , 20 ( 5 ): 255 - 257 .

严京，高健生，接传红，等．密蒙花方改善早期糖尿病视网膜病变中医症状及其用药安全性的研究 [J]. 北京中医药大学学报， 2010 , 33 ( 11 ): 773 - 776 .

严京，高健生，接传红，等．密蒙花方对非增殖期糖尿病视网膜眼底病变影响的研究 [J]. 世界中西医结合杂志， 2013 , 8 ( 3 ): 246 - 248 .

接传红，高健生，严京，等．密蒙花方对单纯型糖尿病视网膜病变患者视网膜功能的影响 [J]. 中国中医眼科杂志， 2013 , 23 ( 3 ): 157 - 160 .

接传红，高健生，严京，等．密蒙花方对单纯型糖尿病视网膜病变患者视网膜功能的影响 [J]. 中国中医眼科杂志， 2010 , 20 ( 6 ): 323 - 325 .

CHEN M X , LV H B , GAN J K , et al . Tang wang ming mu granule attenuates diabetic retinopathy in type 2 diabetes rats [J]. Front Physiol , 2017 , doi: 10.3389/fphys.2017.01065 doi:10.3389/fphys.2017.01065 .

张利青，张占刚，付岩，等．桂皮醛药理作用的研究进展 [J]. 中国中药杂志， 2015 , 40 ( 23 ): 4568 - 4572 .

黄链莎，刘铜华，孙文，等．桂皮醛对糖尿病小鼠血糖水平的影响及机制 [J]. 中国实验方剂学杂志， 2018 , 24 ( 10 ): 95 - 100 .

李秋霞，吴凝，聂闪闪，等．葛根交泰丸对2型糖尿病大鼠的治疗作用 [J]. 中国实验方剂学杂志， 2018 , 24 ( 7 ): 133 - 138 .

王刚，张凯伦．人脐静脉内皮细胞的研究和应用 [J]. 医学综述， 2011 , 17 ( 4 ): 972 - 975 .

宋姗姗，刘明华．血管内皮生长因子与肿瘤血管生成的研究进展 [J]. 四川生理科学杂志， 2010 , 32 ( 4 ): 167 - 169 .

Storkebaum E , Lambrechts D , Carmeliet P. VEGF: once regarded as a specific angiogenic factor, now implicated in neuroprotection [J]. Bioessays , 2004 , 26 ( 9 ): 943 - 954 .

游逸安，许雯怡，朱乐如．增殖性糖尿病视网膜病变玻璃体与血清SDF-1、VEGF含量分析 [J]. 医学研究杂志， 2013 , 42 ( 1 ): 100 - 104 .

Olsson A K , Dimberg A , Kreuger J , et al . VEGF receptor signaling in control of vascular function [J]. Nat Rev Mol Cell Biol , 2006 , 7 ( 5 ): 359 - 371 .

韩琳．麝香保心丸中促血管新生活性的药效物质及其作用机制研究 [D]．上海：第二军医大学， 2014 .

石鑫超，吴忌，王庆甫，等．桂皮醛对人膝骨关节炎滑膜成纤维细胞增殖的影响 [J]. 中国中医骨伤科杂志， 2014 , 22 ( 9 ): 1 - 3 .

郑志．活性氧在糖尿病性视网膜病变中作用机制及干预研究 [D]．上海：上海交通大学， 2007 .

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