Analysis of Chemical Constituents of Volatile Oils in Menthae Haplocalycix Herba- Schizonepetae Herba and Herbal Pairs and Comparison of Anti-bacterial Effects <italic style="font-style: italic">in Vitro</italic>

Ya-chun SHU; Yu DUAN; Ya-jun CHEN; Kai-lei FAN; Hong SUN; Jing LIU; Hao CAI

doi:10.13422/j.cnki.syfjx.20190714

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Analysis of Chemical Constituents of Volatile Oils in Menthae Haplocalycix Herba- Schizonepetae Herba and Herbal Pairs and Comparison of Anti-bacterial Effects in Vitro

Compatibility | 更新时间：2021-02-09

- Analysis of Chemical Constituents of Volatile Oils in Menthae Haplocalycix Herba- Schizonepetae Herba and Herbal Pairs and Comparison of Anti-bacterial Effects in Vitro
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 6-13(2019)
- 作者机构：
  
  1.南京中医药大学　附属江苏省中医院，南京　 210029；
  2.南京中医药大学　国家教育部中药炮制规范化及标准化工程研究中心，南京　 210023；
  3.南京医科大学　附属妇产医院，南京　 210004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190714
  CLC： R284.1;R289;R22;R2-031
- Received：31 May 2018，
  
  Published Online：18 December 2018，
  
  Published：05 April 2019
- 稿件说明：
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Ya-chun SHU, Yu DUAN, Ya-jun CHEN, et al. Analysis of Chemical Constituents of Volatile Oils in Menthae Haplocalycix Herba- Schizonepetae Herba and Herbal Pairs and Comparison of Anti-bacterial Effects in Vitro[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 6-13.
DOI：

Ya-chun SHU, Yu DUAN, Ya-jun CHEN, et al. Analysis of Chemical Constituents of Volatile Oils in Menthae Haplocalycix Herba- Schizonepetae Herba and Herbal Pairs and Comparison of Anti-bacterial Effects in Vitro[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 6-13. DOI： 10.13422/j.cnki.syfjx.20190714.

摘要

目的：

研究薄荷、荆芥配伍前后挥发油成分变化及体外抑菌作用比较，为薄荷-荆芥药对物质基础研究提供实验依据。

方法：

分别用水蒸气蒸馏法提取薄荷-荆芥药对及薄荷、荆芥单味饮片挥发油，通过GC-MS对其挥发性成分进行对比分析，并进行体外抑菌作用比较。

结果：

在薄荷挥发油中共鉴定出50种化合物，在荆芥挥发油中共鉴定出41种化合物，在薄荷-荆芥药对挥发油中共鉴定出60种化合物。3种挥发油样品共有化合物27种，薄荷-荆芥药对挥发油中新测得5种化合物，9种化合物未检测到。在体外抑菌作用上，薄荷-荆芥药对及其单味药挥发油对粪肠球菌(ATCC2922)，金黄色葡萄球菌(ATCC2923)，大肠埃希菌(ATCC35218)，大肠埃希菌(ATCC25922)均有抑菌作用，其中薄荷-荆芥药对挥发油对粪肠球菌(ATCC2922)和金黄色葡萄球菌(ATCC2923)的抑菌作用强于薄荷、荆芥单味药挥发油，对大肠埃希菌(ATCC35218和ATCC25922)的抑制作用，药对挥发油＝薄荷挥发油>荆芥挥发油。

结论：

薄荷-荆芥药对挥发油成分组成和相对含量较薄荷、荆芥单味药均有一定变化，薄荷-荆芥药对挥发油的体外抑菌作用略优于单味药挥发油。

Abstract

Objective:

To discuss the effect of herbal compatibility on the components of volatile oils in Menthae Haplocalycix Herba and Schizonepetae Herba

and the comparison of the anti-bacterial effects

in vitro

in order to provide the experimental basis for investigating the effective material basis of their herb-pair.

Method:

Steam distillation was used to extract volatile oils of Menthae Haplocalycix Herba

Schizonepetae Herba

and their combination. GC-MS was employed to analyze their volatile oils

and the anti-bacterial effects

in vitro

were compared.

Result:

Totally 50 compounds were identified from the volatile oils of Menthae Haplocalycix Herba; 41 compounds were identified from the volatile oils of Schizonepetae Herba; and 60 compounds were identified from the volatile oils of their herbal pairs. A total of 27 common compounds were found in the samples of three volatile oils. Totally 5 new compounds were detected in the volatile oils of their herbal pairs

and 9 compounds were not detected.

In vitro

volatile oils of both their herbal pairs and individual herbs had bacteriostatic effects on dung

Enterococcus

(ATCC2922)

Staphylococcus aureus

(ATCC2923)

Escherichia coli

(ATCC35218)

and

coli

(ATCC25922). Among them

volatile oils of their herbal pairs had stronger bacteriostatic effects on dung enterococcus (ATCC2922) and staphylococcus aureus (ATCC2923) than volatile oils of individual herbs. Moreover

the order of the inhibitory effects of volatile oils on

coli

(ATCC35218 and ATCC25922) were "herbal pairs=peppermint

herba schizonepetae" .

Conclusion:

There were some changes between the volatile oils of individual herbs and the volatile oils of their herbal pairs. These phenomena may be caused by the occurrence of certain chemical reactions and physical changes in compatibility of decocting process. The antibacterial effects of volatile oils of their herbal pairs were better than those of individual herbs

in vitro

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Keywords

references

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Analysis of Chemical Constituents of Volatile Oils in Menthae Haplocalycix Herba- Schizonepetae Herba and Herbal Pairs and Comparison of Anti-bacterial Effects in Vitro

DOI：10.13422/j.cnki.syfjx.20190714

摘要

Abstract

关键词

Keywords

references