Analysis of Rhizosphere Soil Fungal Community Composition and Diversity of Salvia miltiorrhiza Based on ITS Sequences

Wei LIU; Bing-qian ZHOU; Xiao WANG; Heng LU; Lan-ping GUO; Feng-sheng LI

doi:10.13422/j.cnki.syfjx.20190715

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Analysis of Rhizosphere Soil Fungal Community Composition and Diversity of Salvia miltiorrhiza Based on ITS Sequences

Resource and Quality Evaluation | 更新时间：2021-02-09

- Analysis of Rhizosphere Soil Fungal Community Composition and Diversity of Salvia miltiorrhiza Based on ITS Sequences
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 9, Pages: 130-135(2019)
- 作者机构：
  
  1.齐鲁工业大学（山东省科学院）山东省分析测试中心，山东省中药质量控制技术重点实验室（筹），济南　 250014
  2.中国中医科学院　中药资源中心，中国中医科学院博士后科研流动站，北京　 100700
  3.莱芜紫光生态园有限公司，山东　莱芜　 271100
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190715
  CLC： R284.1; R289; R22; R2-031
- Received：28 May 2018，
  
  Published Online：21 December 2018，
  
  Published：05 May 2019
- 稿件说明：
移动端阅览
Wei LIU, Bing-qian ZHOU, Xiao WANG, et al. Analysis of Rhizosphere Soil Fungal Community Composition and Diversity of Salvia miltiorrhiza Based on ITS Sequences[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 130-135.
DOI：

Wei LIU, Bing-qian ZHOU, Xiao WANG, et al. Analysis of Rhizosphere Soil Fungal Community Composition and Diversity of Salvia miltiorrhiza Based on ITS Sequences[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 130-135. DOI： 10.13422/j.cnki.syfjx.20190715.

摘要

目的：

该文研究了丹参连作根际土壤真菌群落组成及多样性变化，有利于阐述丹参-土壤-真菌根际微环境的变化，为揭示连作障碍形成的原因提供理论基础。

方法：

基于高通量测序技术，分别对未种植丹参(CK)，连作1年(Y1)及连作两年(Y2)的根际土壤进行转录间隔区(ITS)测序，3种处理中分别得到14 153，19 024及7 622条有效序列，注释为1 027，1 095及712种真菌可操作分类单元(OTU)。

结果：

随着连作年限的增加，真菌群落多样性呈现出先下降后上升的变化趋势。在纲分类水平上，优势菌群分别为伞菌纲(Agaricomycetes)，锤舌菌纲(Leotiomycetes)及银耳纲(Tremellomycetes)，在3种处理中所占比例大小分别为Y1>CK>Y2，Y2>Y1>CK

CK>Y2>Y1；在属分类水平上，CK中优势菌属为耐冷酵母(Guehomyces)，Y1及Y2较CK相比分别下降96.13%和90.21%，异常威克汉姆酵母(Wickerhamomyces)在Y2中所占比例增幅明显(6.64%)，较CK及Y1相比分别增加290.59%及564.00%。

结论：

CK及Y1样品中根际土壤真菌在属水平上群落结构上具有更高的相似性，Y2样品群落结构分布热图显示炭疽菌属(

Cryptosporiopsis

)等致病真菌成为优势菌属，推测与连作丹参病害率高有一定相关性。研究结果为阐述丹参连作障碍形成、发展绿色种植提供理论支撑。

Abstract

Objective:

To study rhizosphere soil fungal community composition and diversity of

Salvia miltiorrhiza

in order to explain the changes of the rhizosphere microenvironment of

miltiorrhiza

and provide theoretical basis for revealing the reasons for the formation of continuous obstacles.

Method:

Based on the high-throughput sequencing technology

three kinds of soil samples were collected

namely soil of non-plant (CK)

soil for cultivating for one year (Y1) and soil for cultivating for two years (Y2)

respectively. According to the sequencing of the internal transcribed space of nuclear ribosomal DNA(ITS) region of fungal rRNA gene

14 153

19 024 and 7 622 valid sequences were obtained and annotated as 1 027

1 095 and 712 varieties of OTUs. The diversity of fungal communities showed a trend of decreasing first and then increasing. Agaricomycetes

Leotiomycetes and Tremellomycetes were the dominant bacterial communities at the class level

their orders in three treatments were Y1>CK>Y2

Y2>Y1>CK

CK>Y2>Y1.At the genus level

Guehomyces was dominant species in CK

and both Y1 and Y2 had a decrease of 96.13%and 90.21%

respectively. Wickerhamomyces had a significantly increase in Y2 (6.64%)

250.59%and 564.00%compared with CK and Y1

respectively.

Result:

The results of cluster analysis showed that CK and Y1 had a higher similarity at the genus level in soil fungal community composition compared with Y2.

Conclusion:

Cryptosporiopsis

and other pathogenic fungal communities were dominant genuses according to the heatmap analysis in Y2

suggesting a correlation with the incidence of

miltiorrhiza

. The findings provide theoretical basis for the formation of continuous cropping obstacle.

关键词

Keywords

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