Fingerprint of Epimedium wushanense Based on Principal Component,Factor and Cluster Analysis

Yue-yun WANG; Xue-fei WANG; Jiao LIU; Wen-fen XU

doi:10.13422/j.cnki.syfjx.20190717

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Fingerprint of Epimedium wushanense Based on Principal Component,Factor and Cluster Analysis

Resource and Quality Evaluation | 更新时间：2021-02-09

- Fingerprint of Epimedium wushanense Based on Principal Component,Factor and Cluster Analysis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 165-172(2019)
- 作者机构：
  
  贵阳中医学院　药学院，贵阳　 550002
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190717
  CLC： R284.1;R289;R22;R2-031
- Received：10 May 2018，
  
  Published Online：19 December 2018，
  
  Published：05 April 2019
- 稿件说明：
移动端阅览
Yue-yun WANG, Xue-fei WANG, Jiao LIU, et al. Fingerprint of Epimedium wushanense Based on Principal Component,Factor and Cluster Analysis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 165-172.
DOI：

Yue-yun WANG, Xue-fei WANG, Jiao LIU, et al. Fingerprint of Epimedium wushanense Based on Principal Component,Factor and Cluster Analysis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 165-172. DOI： 10.13422/j.cnki.syfjx.20190717.

摘要

目的：

采用HPLC建立巫山淫羊藿的指纹图谱，结合主成分、因子和聚类分析对不同产地巫山淫羊藿综合品质进行研究，为其质量评价提供理论基础。

方法：

采用Agilent InfinityLab Poroshe1l 120 SB-C

色谱柱（3.0 mm×100 mm，2.7 μm），流动相乙腈（A）-水（B）梯度洗脱（0～5 min，25%～26%A；5～6 min，26%～34%A；6～11 min，34%～38.5%A；11～17 min，38.5%～100%A；17～20 min，100%A），流速0.8 mL·min

－1

，检测波长270 nm，柱温30 ℃。

结果：

聚类分析较好地对不同产地的巫山淫羊藿进行分类，贵州产巫山淫羊藿和重庆巫山淫羊藿在聚类分析中明显分为二类，相距较远，说明由于环境气候的影响，不同产地的淫羊藿在品质上存在差异。主成分、因子分析结果，重庆巫山淫羊藿质量总体优于贵州产巫山淫羊藿，其中CQWS-02（重庆市巫山县官阳镇鸦鹊村），CQWS-10（重庆市巫山县官阳镇河湾）两个产地的样品在优质品种筛选时可重点考虑。另外，1号（朝藿定A），2号（朝藿定B），4号（淫羊藿苷），5号（未知成分）共有峰可作为巫山淫羊藿质量评价指标成分。

结论：

通过主成分、因子和聚类分析，可实现快速分析，发挥各自优势，互相验证和补充，多方面综合评判不同产地巫山淫羊藿药材的质量。

Abstract

Objective:

To establish the fingerprint of

Epimedium wushanense

by HPLC

and study the comprehensive quality of

wushanense

by combining principal components

factors and cluster analysis

so as to provide theoretical basis for its quality evaluation.

Method:

The chromatographic column was Agilent infinitylab poroshell 120 SB-C

(3.0 mm× 100 mm

2.7 μm)

the flow phase was acetonitrile (A)-water (B) with a gradient of 0-5 min

25%-26%A; 5-6 min

26%-34%A; 6-11 min

34%-38.5%A; 11-17 min

38.5%-100%A; 17-20 min

100%A

the flow rate was 0.8 mL·min

- 1

the detection wavelength was 270 nm

and the column temperature was 30 ℃.

Result:

The cluster analysis better classified

wushanense

from different producing areas.

wushanense

from Guizhou province and

wushanense

from Chongqing were classified as class Ⅱ.

wushanense

from Guizhou province and Chongqing were far apart

indicating that the quality of

wushanense

varies from place to place affected by environment and climate. The results of principal component analysis showed that the quality of

wushanense

produced in Chongqing was better than that of

wushanense

produced in Guizhou. Among them

CQWS-02 (Yaque village

Guanyang town

Wushan county

Chongqing) and CQWS-10 (Hewan

Guanyang town

Wushan county

Chongqing) can be considered in the selection of high-quality varieties. In addition

No.1 common peak (epimedin A)

No.2 common peak (epimedin B)

No.4 common peak (icariin) and No.5 common peak (unknown component) in the fingerprint of the test samples could be used as the evaluation index components of

wushanense

quality.

Conclusion:

Principal components

factors and cluster analysis are used to achieve the rapid analysis

and their respective advantages are brought into full play for mutual verification and supplement. And the quality of

wushanense

in different origins can be comprehensively evaluated in all-round ways.

关键词

Keywords

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