Effect of Capsaicin on Proliferation and HMGB1 and IL-6 Expressions in Hepatoma SMMC-7721 Cells

Chan-chan XIAO; Mao-jian CHEN; Fan-biao MEI; Guan-hua REN; Tian-ren HUANG; Ji-lin LI; Wei DENG

doi:10.13422/j.cnki.syfjx.20190721

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Effect of Capsaicin on Proliferation and HMGB1 and IL-6 Expressions in Hepatoma SMMC-7721 Cells

Topic on Antitumor Research of Capsaicin | 更新时间：2021-02-09

- Effect of Capsaicin on Proliferation and HMGB1 and IL-6 Expressions in Hepatoma SMMC-7721 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 89-93(2019)
- 作者机构：
  
  广西医科大学　附属肿瘤医院，南宁　 530021
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190721
  CLC： R22;R242;R2-031;R285.5
- Received：12 October 2018，
  
  Published Online：20 December 2018，
  
  Published：05 April 2019
- 稿件说明：
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Chan-chan XIAO, Mao-jian CHEN, Fan-biao MEI, et al. Effect of Capsaicin on Proliferation and HMGB1 and IL-6 Expressions in Hepatoma SMMC-7721 Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 89-93.
DOI：

Chan-chan XIAO, Mao-jian CHEN, Fan-biao MEI, et al. Effect of Capsaicin on Proliferation and HMGB1 and IL-6 Expressions in Hepatoma SMMC-7721 Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 89-93. DOI： 10.13422/j.cnki.syfjx.20190721.

摘要

目的：

观察辣椒碱对肝癌SMMC-7721细胞增殖的影响，并探讨其作用的分子机制。

方法：

设立辣椒碱(50，100，150，200，250，300 μmol·L

－1

)组以及空白组，采用细胞计数试剂盒-8(CCK-8)法检测辣椒碱(50，100，150，200，250，300 μmol·L

－1

)作用SMMC-7721细胞24，48，72 h后的细胞活性；采用光学倒置显微镜观察辣椒碱(150，200，250 μmol·L

－1

)作用SMMC-7721细胞形态的变化；实时荧光定量PCR(Real-time PCR)检测辣椒碱(150，200，250 μmol·L

－1

)作用SMMC-7721细胞24 h后细胞中高迁移率族蛋白B1(HMGB1)和白细胞介素-6(IL-6)mRNA表达水平；采用酶联免疫吸附测定(ELISA)检测辣椒碱(150，200，250 μmol·L

－1

)作用SMMC-7721细胞24 h后细胞培养上清中HMGB1和IL-6的水平。

结果：

与空白组比较，辣椒碱50，100 μmol·L

－1

组作用24，48，72 h差异均无统计学意义；其余辣椒碱(150，200，250，300 μmol·L

－1

)作用不同时间点，增殖抑制率均明显升高(

0.05)，且具有明显的浓度和时间依赖效应；与空白组比较，辣椒碱(150，200，250 μmol·L

－1

)组SMMC-7721细胞均出现不同程度地形态学改变，表现为细胞变圆、皱缩，贴壁差，脱落增多，给药浓度越高，效果就越明显；与空白组比较，辣椒碱(150，200，250 μmol·L

－1

)组SMMC-7721细胞中HMGB1

IL-6 mRNA表达明显下调(

0.05)，上清中HMGB1和IL-6分泌明显降低(

0.05)。

结论：

辣椒碱对肝癌SMMC-7721细胞增殖具有抑制作用，潜在机制可能与其抑制细胞HMGB1和IL-6的表达与分泌有关。

Abstract

Objective:

To investigate the effect of capsaicin on proliferation in human hepatoma SMMC-7721 cells and its possible molecular mechanism.

Method:

Capsaicin (50

100

150

200

250

300 μmol·L

-1

) groups and blank group were set up. The cell viability was detected by cell counting kit-8 (CCK-8) assay after SMMC-7721 cells were treated with capsaicin (50

100

150

200

250

300 μmol·L

-1

) for 24

72 h. The morphological changes were observed under an inverted microscope after SMMC-7721 cells were treated with capsaicin (150

200

250 μmol·L

-1

) for 24 h. The mRNA expression levels of high mobility group box 1 (HMGB1) and interleukin-6(IL-6) were measured by Real-time PCR after SMMC-7721 cells were treated with capsaicin (150

200

250 μmol·L

-1

) for 24 h. The levels of HMGB1 and IL-6 in cell culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA) after SMMC-7721 cells were treated with capsaicin (150

200

250 μmol·L

-1

) for 24 h.

Result:

Compared with the blank group

there was no significant difference between 50 and 100 μmol·L

-1

capsaicin groups treated for 24

72 h; after treated with the other concentrations of capsaicin (150

200

250

300 μmol·L

-1

) at different time points

the proliferation inhibition rate was statistically significant (

0.05)

with significant concentration and time-dependent effect; compared with the blank group

capsaicin (150

200

250 μmol·L

-1

) groups showed different degrees of morphological changes in SMMC-7721 cells

which became round and wrinkled

with a poor attachment and more exfoliation; compared with the blank group

the mRNA expressions of HMGB1 and IL-6 in SMMC-7721 cells of capsaicin (150

200

250 μmol·L

-1

) groups were significantly down-regulated (

0.05)

and the secretions of HMGB1 and IL-6 were significantly decreased in the supernatant (

0.05).

Conclusion:

Capsaicin inhibits cell proliferation of SMMC-7721 cells

and the possible mechanism may be related to the down-regulation of HMGB1 and IL-6 at the mRNA and protein levels.

关键词

Keywords

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