Effect of Fengshi Qutong Capsule on Function of Human Synovial Cells in Rheumatoid Arthritis Induced by TNF-α

Lian-hua HE; Yi-qun LI; Jing-xia WANG; Cong-cong SUN; Chun-fang LIU; Yu JING; Yan-dong MIAO; Na LIN

doi:10.13422/j.cnki.syfjx.20190739

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Effect of Fengshi Qutong Capsule on Function of Human Synovial Cells in Rheumatoid Arthritis Induced by TNF-α

Pharmacology | 更新时间：2021-02-09

- Effect of Fengshi Qutong Capsule on Function of Human Synovial Cells in Rheumatoid Arthritis Induced by TNF-α
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 116-121(2019)
- 作者机构：
  
  1.中国中医科学院　中药研究所，北京　 100700；
  2.北京大学　深圳医院，广东　深圳　 518036；
  3.通化金马药业集团股份有限公司，北京　 100028
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190739
  CLC： R289;R593.22;R446.63
- Received：13 October 2018，
  
  Published Online：24 December 2018，
  
  Published：05 April 2019
- 稿件说明：
移动端阅览
Lian-hua HE, Yi-qun LI, Jing-xia WANG, et al. Effect of Fengshi Qutong Capsule on Function of Human Synovial Cells in Rheumatoid Arthritis Induced by TNF-α[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 116-121.
DOI：

Lian-hua HE, Yi-qun LI, Jing-xia WANG, et al. Effect of Fengshi Qutong Capsule on Function of Human Synovial Cells in Rheumatoid Arthritis Induced by TNF-α[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 116-121. DOI： 10.13422/j.cnki.syfjx.20190739.

摘要

目的：

研究风湿祛痛胶囊(FSQTC)对肿瘤坏死因子-

(TNF-

)诱导的人类风湿关节炎(RA)成纤维样滑膜细胞增殖、迁移、黏附、侵袭和分泌功能的影响。

方法：

TNF-

(20 μg·L

－1

)体外诱导RA患者成纤维样滑膜细胞(MH7A)，加入不同浓度FSQTC(0.02，0.1，0.5 μg·L

－1

)作用后，分别采用噻唑蓝(MTT)比色法，转移小室(transwell)迁移、黏附及侵袭实验检测MH7A细胞的增殖活性、迁移、黏附及侵袭能力，酶联免疫吸附测定(ELISA)检测细胞上清中白细胞介素(IL)-1

及血管内皮细胞生长因子(VEGF)的含量。

结果：

与空白组比较，TNF-

(20 μg·L

－1

)能显著增加MH7A细胞的增殖、迁移、黏附、侵袭能力及分泌IL-1

和VEGF的含量(

0.01)；与TNF-

组比较，FSQTC(0.02，0.1，0.5 μg·L

－1

)作用24 h对TNF-

诱导的MH7A细胞增殖活性没有明显影响，作用48 h能浓度依赖地降低TNF-

诱导增加的MH7A细胞增殖活性(

0.05，

0.01)，作用24 h还能显著降低TNF-

诱导升高的MH7A细胞迁移、黏附、侵袭能力及分泌IL-1

和VEGF的含量(

0.05，

0.01)。

结论：

FSQTC可降低MH7A细胞增殖、迁移、黏附、侵袭及分泌IL-1

和VEGF的能力。

Abstract

Objective:

To study the effects of Fengshi Qutong capsule (FSQTC) on proliferation

migration

adhesion

invasion and secretion of human synovial cells in rheumatoid arthritis (RA) induced by tumor necrosis factor-

(TNF-

) and explore its mechanism.

Method:

Human synovial cells (MH7A) in RA patients were induced

in vitro

by using TNF-

(20 μg·L

-1

). After treatment with different concentrations of FSQTC (0.02

0.1

0.5 μg·L

-1

)

MTT colorimetric assay

transwell migration

adhesion and invasion tests were used to detect the proliferation

migration

adhesion and invasion of the MH7A

respectively. The expression levels of vascular endothelial growth factor (VEGF) and interleukin-1

(IL-1

) in MH7A supernatant were detected by enzyme linked immunosorbent assay (ELISA).

Result:

As compared with blank control group

TNF-

(20 μg·L

-1

) significantly increased the proliferation

migration

adhesion

invasion and secretion of IL-1

and VEGF of MH7A cells (

0.01). FSQTC (0.02

0.1

0.5 μg·L

-1

) had no significant effect on proliferation of TNF-

-induced MH7A cells after treatment for 24 hours. After 48 hours of treatment

proliferation of MH7A cells induced by TNF-

was decreased in a concentration-dependent manner (

0.05

0.01). Within 24 hours

the migration

adhesion

invasion

and secretion of IL-1 and VEGF in MH7A cells were also decreased significantly (

0.05

0.01).

Conclusion:

FSQTC can inhibit the proliferation

migration

adhesion

invasion and secretion of IL-1

and VEGF in MH7A cells.

关键词

Keywords

references

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