Callus Induction from Amomum villosum

Ming-xiao LI; Shu-hui CHEN; Jing SU; Li-yun TANG; Jie XU; Zhuo-hang HE; Guo-zhen HE

doi:10.13422/j.cnki.syfjx.201907819

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Callus Induction from Amomum villosum

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- Callus Induction from Amomum villosum
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 8, Pages: 114-119(2019)
- 作者机构：
  
  1.广州中医药大学　中药学院，广州　 510006；
  2.浙江施强制药有限公司，杭州　 310000；
  3.阳春市砂仁试验示范场，广东　阳春　 529600；
  4.华南农业大学　生命科学学院，广州　 510642；
  5.广东一方制药有限公司，广东　佛山　 528200；
  6.广州中医药大学，岭南中药资源教育部重点实验室，广州　 510006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.201907819
  CLC： R284.2;R289;R22;R2-031
- Received：05 October 2018，
  
  Published Online：05 January 2019，
  
  Published：20 April 2019
- 稿件说明：
移动端阅览
Ming-xiao LI, Shu-hui CHEN, Jing SU, et al. Callus Induction from Amomum villosum[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 114-119.
DOI：

Ming-xiao LI, Shu-hui CHEN, Jing SU, et al. Callus Induction from Amomum villosum[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 114-119. DOI： 10.13422/j.cnki.syfjx.201907819.

摘要

目的：

通过阳春砂组织培养获得愈伤组织，建立阳春砂愈伤组织的诱导体系。

方法：

将阳春砂根状茎芽及阳春砂试管苗的茎段、根尖作为愈伤组织诱导的外植体材料，接种到以MS为基本培养基，分别添加不同浓度的6-苄基腺嘌呤(6-BA)，ɑ-萘乙酸(NAA)及2

4-二氯苯氧乙酸(2

4-D)的培养基中(各培养基的pH约为5.8)，探讨不同外植体材料及不同培养基对阳春砂愈伤组织诱导的影响。

结果：

研究结果显示，阳春砂根状茎芽及试管苗的茎段、根尖3种外植体材料均能有效地诱导产生愈伤组织；其中阳春砂根状茎芽、试管苗茎段2种外植体材料诱导产生愈伤组织的最适培养基为MS+6-BA(1.5 mg·L

-1

)+2

4-D(1.0 mg·L

-1

)+NAA(0.5 mg·L

-1

)，最高诱导率分别为15%和60%；试管苗根尖诱导产生愈伤组织的最适培养基为MS+6-BA(2.0 mg·L

-1

)+2

4-D(1.0 mg·L

-1

)+NAA(1.0 mg·L

-1

)，最高诱导率76%，为阳春砂愈伤组织诱导的最佳外植体材料。

结论：

该研究初步建立了阳春砂的根状茎芽及试管苗的茎段、根尖3种外植体的愈伤组织诱导体系，试管苗根尖为愈伤组织诱导的最佳外植体。

Abstract

Objective:

To set up a callus induction system for

Amomum villosum

by tissue culture.

Method:

The rhizome buds of

villosum

and stem segments

root tip segments of sterile

villosum

plantles were used as explants and cultured in MS media with different concentrations of 6-BA

NAA and 2

4-D (the pH of each medi is about 5.8). A callus induction system was established to explore the effect of different explants and different medium on callus induction for

villosum

Result:

The findings showed that the rhizome buds and sterile plantlet stems and root tip segments of three different explants can be successfully induced into calli. The most suitable medium for callus induction from rhizome buds and sterile plantlet stems was MS with 6-BA (1.5 mg·L

-1

)

4-D (1.0 mg·L

-1

) and NAA (0.5 mg·L

-1

) with the highest induction rates of 15%and 60%respectively. MS medium combined with 6-BA (2.0 mg·L

-1

)

4-D (1.0 mg·L

-1

) and NAA (1.0 mg·L

-1

) was the most suitable proposal for inducing the callus from sterile root tip segments with the highest induction rate of 76%.

Conclusion:

Under certain culture conditions

rhizome buds

stem or root tip segments of sterile plantlet can be effectively induced into callus. The callus induction system of

villosum

is preliminarily established

and root tip segments of sterile plantlet are the optimal explant.

关键词

Keywords

references

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Related Author

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