Protective Effect of Gastrodiae Rhizoma Powder with Different Particle Sizes on Cerebral Ischemia Reperfusion Injury in Rats

Ting-ting CHEN; Fan LIU; Yan CHEN; Zi-peng GONG; Tao TAO; Ai-min WANG

doi:10.13422/j.cnki.syfjx.20190803

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Protective Effect of Gastrodiae Rhizoma Powder with Different Particle Sizes on Cerebral Ischemia Reperfusion Injury in Rats

Pharmacology | 更新时间：2021-02-09

- Protective Effect of Gastrodiae Rhizoma Powder with Different Particle Sizes on Cerebral Ischemia Reperfusion Injury in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 8, Pages: 71-76(2019)
- 作者机构：
  
  1.贵州医科大学　贵州省药物制剂重点实验室，省部共建药用植物功效与利用国家重点实验室，贵阳　 550004；
  2.贵州医科大学　药学院，贵阳　 550004；
  3.贵州省人民医院，贵阳　 550004；
  4.贵州医科大学　民族药与中药开发应用教育部工程研究中心，贵阳　 550004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190803
  CLC： R2-0;R22;R285.5
- Received：20 August 2018，
  
  Published Online：04 January 2019，
  
  Published：20 April 2019
- 稿件说明：
移动端阅览
Ting-ting CHEN, Fan LIU, Yan CHEN, et al. Protective Effect of Gastrodiae Rhizoma Powder with Different Particle Sizes on Cerebral Ischemia Reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 71-76.
DOI：

Ting-ting CHEN, Fan LIU, Yan CHEN, et al. Protective Effect of Gastrodiae Rhizoma Powder with Different Particle Sizes on Cerebral Ischemia Reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 71-76. DOI： 10.13422/j.cnki.syfjx.20190803.

摘要

目的：

研究不同粒径的天麻超微粉对局灶性脑缺血再灌注损伤大鼠的保护作用，为超微粉技术在天麻相关产品中的应用提供理论依据。

方法：

SD大鼠随机分为假手术组，模型组，天麻超微粉Ⅰ组(1.2 g·kg

-1

)，天麻超微粉Ⅱ组(1.2 g·kg

-1

)，天麻超微粉Ⅲ组(1.2 g·kg

-1

)，阳性药尼莫地平组(12.6 g·kg

-1

)，连续口服给药7 d后造模。采用单侧线栓法闭塞中动脉1 h后再灌注24 h，制备局灶性缺血再灌注损伤模型

5-氯化二苯基四氮唑(TTC)染色法测定脑组织梗死面积，比色法测定脑组织匀浆液和血清中的超氧化物歧化酶(SOD)的活性、丙二醛(MDA)含量、一氧化氮合酶(NOS)活性，液相色谱串联质谱法(LC/MSMS)记录脑海马中谷氨酸(Glu)，天冬氨酸(Asp)，甘氨酸(Gly)含量变化。

结果：

与假手术组比较，模型组出现显著的神经行为学功能缺失体征(

0.01)，脑梗死面积显著增加(

0.01)。与模型组比较，天麻超微粉Ⅰ，Ⅱ，Ⅲ组均能明显改善神经行为学功能(

0.05)，明显降低脑梗死面积比

Glu

Asp

MDA含量和NOS活性(

0.05，

0.01)，明显升高SOD活性和Gly含量(P

0.05)。与天麻超微粉Ⅰ组比较，天麻超微粉Ⅲ组SOD活性和Gly含量明显高于超微粉Ⅰ组(

0.05)

Glu

Asp

MDA含量和NOS活性均明显低于超微粉Ⅰ组(

0.05)，天麻超微粉Ⅱ组则均无显著性变化。

结论：

不同粒径的天麻超微粉均对脑缺血再灌注损伤具有预防保护作用，但天麻超微粉Ⅲ较天麻超微粉Ⅰ，Ⅱ预防保护作用较明显，表明天麻超微粉对大鼠大脑中动脉缺血再灌注的脑保护作用与其粒径大小具有一定相关性。

Abstract

Objective:

To study the protective effect of Gastrodiae Rhizoma ultrafine powder with different particle sizes on focal cerebral ischemia-reperfusion injury in rats

in order to provide theoretical basis for the application of ultrafine powder technology in related products.

Method:

The SD rats were randomly divided into six groups

namely sham-operated group

ischemia-reperfusion group

Gastrodiae Rhizoma ultramicro-powder Ⅰ group(1.2 g·kg

-1

)

Gastrodiae Rhizoma ultramicro-powder Ⅱ group(1.2 g·kg

-1

)

Gastrodiae Rhizoma ultramicro-powder Ⅲ group(1.2 g·kg

-1

)

and nimodipine positive drug group(12.6 g·kg

-1

). The cerebral ischemia-reperfusion model was established after 7 days of drug administration. The focal ischemia reperfusion injury model was prepared by middle cerebral artery occlusion (MCAO) for 1 h followed by reperfusion for 24 h. The infarct size of brain tissue was measured by triphenyltetrazolium Chloride(TTC) staining. The activities of superoxide dismutase (SOD)

malondialdehyde (MDA) and nitricoxide synthase (NOS) in brain homogenate and serum were measured by colorimetry. The contents of glutamate(Glu)

aspartic acid(Asp) and glycine(Gly) in hippocampus were determined by liquid chromatography-tandem mass spectrometry (LC/MSMS).

Result:

The model group showed obvious signs of neurobehavioral deficit (

0.01) compared with the sham operation group. The area ratio of cerebral infarction was decreased significantly

the contents of Glu

Asp

MDA and the activity of NOS were significantly decreased

while the activity of SOD and content of Gly were significantly increased (

0.05

0.01) in Gastrodiae Rhizoma ultramicro-powder Ⅰ

Ⅱ

Ⅲ groups compared with the model group. SOD activity and Gly content in the Gastrodiae Rhizoma ultramicro-powder Ⅲ group were significantly higher than those in the Gastrodiae Rhizoma ultramicro-powder Ⅰ group and the Gastrodiae Rhizoma ultramicro-powder Ⅱ group (

0.05)

and Glu

Asp

MDA content and NOS activity in the Gastrodiae Rhizoma ultramicro-powder Ⅰ group were significantly lower than those in the Gastrodiae Rhizoma ultramicro-powder Ⅱ group (

0.05).

Conclusion:

Gastrodiae Rhizoma ultramicro-powder with different particle sizes has a protective effect on cerebral ischemia-reperfusion injury

and the Gastrodiae Rhizoma ultramicro-powder Ⅲ group has a more obvious protective effect than the the Gastrodiae Rhizoma ultramicro-powder Ⅰ group. The results showed that the protective effect of Gastrodiae Rhizoma ultramicro-powder on middle cerebral artery ischemia-reperfusion in rats was related to its particle size.

关键词

Keywords

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