Effect of Rosae Chinensis Flos Total Flavones on Focal Cerebral Ischemia-reperfusion in Rats

Yan LI; Ya-gang SONG; Ming-san MIAO; Shuai SHAO

doi:10.13422/j.cnki.syfjx.20190804

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Effect of Rosae Chinensis Flos Total Flavones on Focal Cerebral Ischemia-reperfusion in Rats

Pharmacology | 更新时间：2021-02-09

- Effect of Rosae Chinensis Flos Total Flavones on Focal Cerebral Ischemia-reperfusion in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 12, Pages: 64-70(2019)
- 作者机构：
  
  1.天津中医药大学，天津　 300193；
  2.河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190804
  CLC： R2-0; R22; R285.5
- Received：27 August 2018，
  
  Published Online：03 January 2019，
  
  Published：20 June 2019
- 稿件说明：
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Yan LI, Ya-gang SONG, Ming-san MIAO, et al. Effect of Rosae Chinensis Flos Total Flavones on Focal Cerebral Ischemia-reperfusion in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 64-70.
DOI：

Yan LI, Ya-gang SONG, Ming-san MIAO, et al. Effect of Rosae Chinensis Flos Total Flavones on Focal Cerebral Ischemia-reperfusion in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 64-70. DOI： 10.13422/j.cnki.syfjx.20190804.

摘要

目的：

通过研究月季花总黄酮对大鼠局灶性脑缺血再灌注模型的影响，探讨其作用机制。

方法：

将大鼠随机分为假手术组、模型组、月季花总黄酮高、中、低剂量组(200，100，50 mg·kg

－1

)及阳性组[尼莫地平组(20 mg·kg

－1

)，脑络通组(500 mg·kg

－1

)]。预先连续给药7 d，末次给药1 h后，复制大鼠大脑中动脉局灶性栓塞(MCAO)模型。造模2 h后再灌注22 h，对死亡率、神经功能缺失评分，检测血清中S-100

；脑组织中丙二醛(MDA)，超氧化物歧化酶(SOD)，一氧化氮(NO)，一氧化氮合酶(NOS)，肿瘤坏死因子-

(TNF-

)，白细胞介素-1

(IL-1

)，细胞间黏附分子-1(ICAM-1)，三磷酸腺苷(ATP)酶；并观察脑组织形态学改变。

结果：

大鼠局灶性脑缺血再灌注模型复制成功。与模型组比较，月季花总黄酮高、中、低剂量组均可显著降低大鼠神经功能缺失的评分(

0.01)，显著降低血清中的S-100

含量(

0.01)，显著降低脑组织中MDA，NO，NOS活性(

0.01)，显著升高脑组织中SOD活性(

0.01)，显著升高脑组织中Na

＋

-ATP酶，Mg

2＋

-ATP酶，Ca

2＋

-ATP酶活性(

0.01)，显著降低脑组织中的TNF-

，IL-1

，ICAM-1含量(

0.01)；显著改善脑组织的损伤(

0.01)。

结论：

月季花总黄酮对大鼠脑缺血再灌注损伤具有保护作用，其作用机制可能与其抗自由基，减轻脑组织的炎症反应及改善脑缺血再灌注损伤后脑组织的能量代谢有关。

Abstract

Objective:

To study the effect of Rosae Chinensis Flos total flavones(RCTF) on the focal cerebral ischemia-reperfusion model in rats

in order to preliminarily explore the mechanism of action.

Method:

Rats were randomly divided into sham-operated group

model group

large

medium

and low-dose RCTF group(200

100

50 mg·kg

-1

) and positive group [Nimodipine group(20 mg·kg

-1

) and Naoluotong group (500 mg·kg

-1

)] . After 7 days of continuous administration

1 hour later after the last administration

the middle cerebral artery middle cerebral artery occlusion (MCAO) model was duplicated. After 2 hours of modeling

perfusion was performed for 22 hours. Mortality and neurological deficits were scored. Serum S-100

was detected; brain tissue malondialdehyde(MDA)

superoxide dismutase (SOD)

nitric oxide (NO)

nitric oxide synthase (NOS)

tumour necrosis factor-

(TNF-

)

interleukin-1

(IL-1

)

intercellular adhesion molecule-1(ICAM-1)

adenosine triphosphate (ATP)ase were measured. The brain tissue morphological changes were observed.

Result:

The rat model of focal cerebral ischemia and reperfusion was successfully replicated. Compared with the model group

RCTF in large

medium

and low-dose RCTF group significantly decreased the score of neurological deficit in rats (

0.01)

significantly decreased the content of S-100

in serum (

0.01)

significantly reduce the levels of MDA

NO and NOS in brain tissue (

0.01)

significantly increase the level of SOD in brain tissue (

0.01)

and significantly increase Na

-ATPase

and Ca

in brain tissue (

0.01)

significantly reduced TNF-

content

IL-1

ICAM-1 content in brain tissue (

0.01)

and significantly improved brain tissue damage (

0.01).

Conclusion:

RCTF have a protective effect on cerebral ischemia-reperfusion injury in rats. The mechanism may be related to the resistance of anti-free radicals

the reduction of inflammation in brain tissue and the improvement of brain energy metabolism after cerebral ischemia reperfusion injury.

关键词

Keywords

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