Simultaneous Determination of Seven Constituents in Qinggan Lidan Mixture by HPLC

Yu-xia LOU; Xiang-yang LI; Zhen-guo LI

doi:10.13422/j.cnki.syfjx.20190817

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Simultaneous Determination of Seven Constituents in Qinggan Lidan Mixture by HPLC

Resource and Quality Evaluation | 更新时间：2021-02-09

- Simultaneous Determination of Seven Constituents in Qinggan Lidan Mixture by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 8, Pages: 128-132(2019)
- 作者机构：
  
  1.河南中医药大学，郑州　 450046;
  2.河南省食品药品检验所，郑州　 450018
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190817
  CLC： R282.2;R289;R22;R2-031
- Received：19 September 2018，
  
  Published Online：14 January 2019，
  
  Published：20 April 2019
- 稿件说明：
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Yu-xia LOU, Xiang-yang LI, Zhen-guo LI. Simultaneous Determination of Seven Constituents in Qinggan Lidan Mixture by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 128-132.
DOI：

Yu-xia LOU, Xiang-yang LI, Zhen-guo LI. Simultaneous Determination of Seven Constituents in Qinggan Lidan Mixture by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 128-132. DOI： 10.13422/j.cnki.syfjx.20190817.

摘要

目的：

建立HPLC同时测定清肝利胆口服液中栀子苷，绿原酸，新绿原酸，隐绿原酸，异绿原酸A

C的含量，为清肝利胆口服液的质量控制提供参考。

方法：

采用Luna C

色谱柱(4.6 mm×250 mm

5 μm)，流动相乙腈-0.4%磷酸溶液，梯度洗脱(0～10 min

8%～12%A；10～30 min

12%A；30～60 min

12%～35%A)，流速1.0 mL·min

-1

，柱温35 ℃，检测波长238

327 nm。

结果：

7种待测成分实现完全分离，并与其他成分达到良好地分离；栀子苷，绿原酸，新绿原酸，隐绿原酸，异绿原酸A

C的线性范围分别在0.188～2.355

0.083～1.040

0.074～0.920

0.075～0.940

0.064～0.800

0.076～0.955

0.071～0.888 μg(

均≥0.999 0)；平均加样回收率分别为99.45%

98.45%

99.06%

98.50%

98.16%

101.01%

96.93%

RSD分别为0.5%

1.8%

1.3%

2.4%

2.3%

1.6%

1.6%，栀子苷，绿原酸，新绿原酸，隐绿原酸，异绿原酸A

C在样品中的质量浓度分别处于3.420～3.794

0.835～0.890

1.222～1.275

1.064～1.210

0.377～0.398

0.419～0.464

0.362～0.405 g·L

-1

。

结论：

该方法多种成分同时测定，简便准确，重复性好，灵敏度高，可用于清肝利胆口服液的质量控制。

Abstract

Objective:

To establish an HPLC method for simultaneous determination of geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C in Qinggan Lidan mixture

in order to provide references for its quality control.

Method:

The analysis of methanol extract of this drug was performed on a 35 ℃ Luna C

column (4.6 mm×250 mm

5μm)

with the mobile phase comprised of acetonitrile-0.4%phosphoric acid flowing at 1.0 mL·min

-1

in a gradient elution mode (0-10 min

8%-12%A; 10-30 min

12%A; 30-60 min

12%-35%A)

and the detection wavelengths were set at 238 and 327 nm.

Result:

Geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C were completely separated

and well separated from other constituents. The linear ranges of geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C were 0.188-2.355

0.083-1.040

0.074-0.920

0.075-0.940

0.064-0.800

0.076-0.955

0.071-0.888 μg (

≥0.999 0)

respectively. The average recoveries were 99.45%

98.45%

99.06%

98.50%

98.16%

101.01%

96.93%

with the RSDs of 0.5%

1.8%

1.3%

2.4%

2.3%

1.6%

respectively.The contents of geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C were 3.420-3.794

0.835-0.890

1.222-1.275

1.064-1.210

0.377-0.398

0.419-0.464 and 0.362-0.405 g·L

-1

respectively.

Conclusion:

This method can be used for simultaneous determination of muti-ingredients in Qinggan Lidan mixture

and the established method is simple and accurate

with a good reproducibility and high sensitivity. It can be used for the quality control of Qinggan Lidan mixture.

关键词

Keywords

references

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