Quantitative and Fingerprint of Ilex pubescens Slices

Hong-xing MA; Long-fei LIN; Yu-ling LIU; Sai FU; Jin-xin SHAO; Ji-zhong ZHU; Chuan-gui LIU; Hui LI; Wen-cong LIU

doi:10.13422/j.cnki.syfjx.20190818

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Quantitative and Fingerprint of Ilex pubescens Slices

Resource and Quality Evaluation | 更新时间：2021-02-09

- Quantitative and Fingerprint of Ilex pubescens Slices
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 13, Pages: 140-150(2019)
- 作者机构：
  
  1.吉林农业大学，长春　 130118；
  2.中国中医科学院　中药研究所，北京　 100700；
  3.承德医学院，河北　承德　 067000；
  4.吉林华康药业股份有限公司，吉林　敦化　 133700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190818
  CLC： R282.2; R289; R22; R2-031
- Received：25 September 2018，
  
  Published Online：11 January 2019，
  
  Published：05 July 2019
- 稿件说明：
移动端阅览
Hong-xing MA, Long-fei LIN, Yu-ling LIU, et al. Quantitative and Fingerprint of Ilex pubescens Slices[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 140-150.
DOI：

Hong-xing MA, Long-fei LIN, Yu-ling LIU, et al. Quantitative and Fingerprint of Ilex pubescens Slices[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 140-150. DOI： 10.13422/j.cnki.syfjx.20190818.

摘要

目的：

建立毛冬青饮片的HPLC-UV指纹图谱，同时测定46批次毛冬青饮片中毛冬青皂苷A

和毛冬青皂苷B

的含量，为毛冬青饮片的质量标准制定提供参考。

方法：

Kromasil C

色谱柱(4.6 mm×250 mm，5 μm)，流动相乙腈-0.1%磷酸水溶液梯度洗脱，流速1.0 mL·min

－1

，检测波长210 nm。采用中药色谱指纹图谱相似度评价系统(2012版)分析毛冬青指纹图谱，SPSS 20.0软件对共有峰的峰面积进行聚类分析；主成分分析法对共有峰进行降维分析。

结果：

毛冬青饮片指纹图谱中根部位与枝部位差异较大，分别建立毛冬青根和枝的指纹图谱，并对共有峰进行聚类分析，聚类结果将根类毛冬青饮片聚为三类，枝类毛冬青饮片聚为二类；对比不同部位及不同产地间毛冬青饮片的整体性和差异性，主成分分析筛选出毛冬青饮片指纹图谱中起决定性作用的共有成分；建立毛冬青皂苷A

，毛冬青皂苷B

含量测定方法。

结论：

建立的毛冬青饮片指纹图谱和含量测定方法操作简便，适用性好，聚类分析和主成分分析筛选出毛冬青饮片质量控制的关键成分，研究结果可为毛冬青饮片质量控制提供参考。

Abstract

Objective:

To establish HPLC-UV fingerprints of

Ilex pubescens

pieces

and simultaneously determine two components in 46 batches of

pubescens

in pieces of

pubescens

saponin A

and B

in order to provide a reference for the quality standard of

pubescens

slices.

Method:

Methanol was used to extract the

pubescens

saponin samples

and the extracts were measured by HPLC-UV with the absorption wavelength at 210 nm. Kromasil C

column (4.6 mm×250 mm

5 μm) was used for determining the extracts at a flow rate of 1.0 mL·min

-1

. The mobile phase condition was acetonitrile-0.1%phosphoric acid aqueous solution with gradient mode. The chromatographic fingerprint similarity evaluation system of traditional Chinese medicine (2012 edition) was used to analyze

pubescens

fingerprints. SPSS 20.0 software was used to cluster the peak area of common peaks. Principal component analysis was performed to reduce the dimension of common peaks.

Result:

There were great differences between the root and stem parts in

pubescens

fingerprints. The fingerprints of roots and stems of

pubescens

were established respectively

cluster results assorted the roots of

pubescens

into three categories andthe branches of

pubescens

into two categories. The integrity and difference of

pubescens

decoction pieces from different parts and places of origin were compared

and the principal component analysis was performed to screen out the common components that played a decisive role in fingerprint of

pubescens

pieces. And the common peaks were determined. The content of saponin A

and saponin B

in Radix

pubescens

were determined.

Conclusion:

The established

pubescens

fingerprints and content determination methods are simple and suitable. Cluster analysis and principal component analysis are used to screen out the key components of quality control of

pubescens

. The results can provide references for quality control of

pubescens

关键词

Keywords

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