Effect of Yiqi Huoxue Recipe on Vascular Endothelial Function in Rats with Qi Deficiency and Blood Stasis Syndrome

Ting-ting HUANG; Xiao HAN; Jian-xun LIU; Ya-hong SHI; Lei LI; Chun-yuan YIN; Jun-guo REN

doi:10.13422/j.cnki.syfjx.20190838

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Effect of Yiqi Huoxue Recipe on Vascular Endothelial Function in Rats with Qi Deficiency and Blood Stasis Syndrome

Pharmacology | 更新时间：2021-02-09

- Effect of Yiqi Huoxue Recipe on Vascular Endothelial Function in Rats with Qi Deficiency and Blood Stasis Syndrome
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 8, Pages: 40-46(2019)
- 作者机构：
  
  1.北京中医药大学　研究生院，北京　 100029；
  2.中国中医科学院　西苑医院　基础医学研究所，中药药理北京市重点实验室，北京　 100091
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190838
  CLC： R289;R241.8;R714.252;R331.3
- Received：13 November 2018，
  
  Published Online：04 January 2019，
  
  Published：20 April 2019
- 稿件说明：
移动端阅览
Ting-ting HUANG, Xiao HAN, Jian-xun LIU, et al. Effect of Yiqi Huoxue Recipe on Vascular Endothelial Function in Rats with Qi Deficiency and Blood Stasis Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 40-46.
DOI：

Ting-ting HUANG, Xiao HAN, Jian-xun LIU, et al. Effect of Yiqi Huoxue Recipe on Vascular Endothelial Function in Rats with Qi Deficiency and Blood Stasis Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 40-46. DOI： 10.13422/j.cnki.syfjx.20190838.

摘要

目的：

探讨气虚血瘀证大鼠血管内皮细胞功能变化及益气活血方(YQHXF)对其的影响。

方法：

实验分为空白组，气虚血瘀组，YQHXF高、低剂量组(5.54

2.77 g·kg

-1

)。采用水环境小平台站立长期无规律、不完全的睡眠剥夺法，每天剥夺16 h，持续6周，制造大鼠神劳加体劳的双重消耗建立大鼠气虚血瘀证候模型，自第5周开始灌胃给药，连续给药2周至实验结束。酶联免疫吸附测定(ELISA)检测大鼠血浆中内皮素-1(ET-1)，血管性血友病因子(vWF)，血管细胞黏附分子-1(VCAM-1)，细胞间黏附分子(ICAM-1)，P选择素，白细胞介素-6(IL-6)，肿瘤坏死因子-

(TNF-

)含量，硝酸还原酶法检测一氧化氮(NO)水平。

结果：

与空白组比较，气虚血瘀组大鼠呈现毛发粗糙晦暗，体质量、脉搏幅度显著降低(

0.01)，舌面R

B值显著降低(

0.01)，全血黏度高、中切值，血浆黏度均显著增高(

0.01)

ET-1，vWF

VCAM-1

ICAM-1

P选择素

IL-6

TNF-

等血管内皮功能相关指标明显升高(

0.05，

0.01)；与气虚血瘀组比较，YQHXF高、低剂量可显著升高气虚血瘀证大鼠脉搏幅度及舌面R

B值(

0.01)，明显降低全血黏度高、中、低切值及血浆黏度(

0.05，

0.01)，同时明显降低大鼠ET-1

IL-6，VCAM-1

P选择素水平(

0.05，

0.01)。

结论：

睡眠剥夺法可诱导大鼠气虚血瘀证形成，并可导致血管内皮功能失调。YQHXF具有保护血管内皮的功能，通过调节血管内皮活性物质分泌、减少细胞黏附、抑制炎症等途径改善气虚血瘀证大鼠气虚及血瘀症状。

Abstract

Objective:

To explore the changes and benefits of vascular endothelial cell function in rats with qi deficiency and blood stasis syndrome

and the effect of Yiqi Huoxue recipe (YQHXF) of such changes.

Method:

Rats were randomly divided into blank control group

Qi deficiency and blood stasis group

and YQHXF high and low dose groups (5.54

2.77 g·kg

-1

). A small platform of water environment was used to make the rats stand for a long-term with irregular and incomplete sleep deprivation

16 h per day for six weeks

so that both mentality and labor of rats were consumed to establish qi deficiency and blood stasis rat models. From the fifth week

intragastric administration was given for 2 weeks

until end of the experiment. Then levels of endothelin-1(ET-1)

von willebrand factor (vWF)

vascular cell adhesion molecule-1(VCAM-1)

intercellular adhesion molecule (ICAM-1)

P-selectin

interleukin-6 (IL-6)

and tumor necrosis factor-

(TNF-

) in rat plasma were detected by enzyme-linked immunosorbent assay (ELISA) and nitric oxide (NO) was detected by nitrate reductase assay.

Result:

Compared with blank control group

rats in Qi deficiency and blood stasis group showed rough and dark hair

with significantly decreased body weight and pulse amplitude (

0.01); R

B values of the tongue surface were decreased significantly (

0.01); high and moderate shear values of whole blood viscosity

as well as plasma viscosity were significantly increased (

0.01)

and the indexes of vascular endothelial function such as ET-1

vWF

VCAM-1

ICAM-1

P-selectin

IL-6 and TNF-

were abnormally increased after sleep deprivation (

0.05

0.01). High and low dose YQHXF significantly increased the pulse amplitude and tongue R

B values(

0.01)

reduced the high

moderate and low shear values of whole blood viscosity

as well as plasma viscosity (

0.05

0.01); at the same time

the levels of ET-1

IL-6

VCAM-1 and P-selectin in rats were decreased (

0.05

0.01).

Conclusion:

Sleep deprivation can induce the formation of Qi deficiency and blood stasis syndrome in rats

and lead to vascular endothelial dysfunction. YQHXF has the function of protecting the vascular endothelium. It can improve the Qi deficiency and blood stasis symptoms in rats with Qi deficiency and blood stasis syndrome by regulating the secretion of vascular endothelial active substances

reducing cell adhesion and inhibiting inflammation.

关键词

Keywords

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