Effect of Manual and Machine Decocting on Chemical Constituents in Bazhentang Based on Non-targeted Metabolomics

Jun-yi ZHOU; Yu LIN; Qiong LUO; Yao-zhou HUANG; Na LANG; Xiong WANG; Yong-liang ZHAO

doi:10.13422/j.cnki.syfjx.20190850

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Effect of Manual and Machine Decocting on Chemical Constituents in Bazhentang Based on Non-targeted Metabolomics

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- Effect of Manual and Machine Decocting on Chemical Constituents in Bazhentang Based on Non-targeted Metabolomics
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 17, Pages: 7-13(2019)
- 作者机构：
  
  1.中国中医科学院　中药研究所，北京　 100700；
  2.北京黄尧洲中医研究院有限公司，北京　 100142；
  3.中国中医科学院　中国医史文献研究所，北京　 100700；
  4.中国中医科学院　西苑医院，北京　 100091；
  5.北京市密云区中医医院，北京　 101599
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190850
  CLC： R22; R94; R28; C37; R969.1
- Received：16 November 2018，
  
  Published Online：04 January 2019，
  
  Published：05 September 2019
- 稿件说明：
移动端阅览
Jun-yi ZHOU, Yu LIN, Qiong LUO, et al. Effect of Manual and Machine Decocting on Chemical Constituents in Bazhentang Based on Non-targeted Metabolomics[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(17): 7-13.
DOI：

Jun-yi ZHOU, Yu LIN, Qiong LUO, et al. Effect of Manual and Machine Decocting on Chemical Constituents in Bazhentang Based on Non-targeted Metabolomics[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(17): 7-13. DOI： 10.13422/j.cnki.syfjx.20190850.

摘要

目的：

通过非靶向代谢组学方法研究手工与机器煎煮对八珍汤中化学成分的影响，寻找这2种不同煎药方式之间的差异性化学成分。

方法：

以规范化手工煎药与机器煎药2种方式对八珍汤进行煎煮，利用正交偏最小二乘法-判别分析(OPLS-DA)等多元统计方法，结合变量重要性投影(VIP)值及

检验分析2种煎药方式对该复方中化学成分的影响。通过UPLC-LTQ-Orbitrap MS对差异性化合物进行分析，流动相乙腈(A)-0.1%甲酸水溶液(B)梯度洗脱(0～3 min，5%A；3～7 min，5%～8%A；7～9 min，8%～15%A；9～17 min，15%～25%A；17～27 min，25%～35%A；27～29 min，35%～40%A；29～35 min，40%～95%A)，柱温30 ℃，流速0.4 mL·min

－1

；采用电喷雾离子源和正、负离子模式，扫描范围

50～1 500。

结果：

在正、负离子监测模式下，共发现87个差异性成分，通过质谱数据结合文献报道，共识别并鉴定了其中的40个成分，包括洋川芎内酯A，甘草素，阿魏酸等。

结论：

手工与机器煎药获得的八珍汤煎煮液在色泽及化学成分上存在着较为明显的差异。若将二者的优点结合起来，在保持手工煎药优势的基础上，借鉴机器煎药包装药液的方式，建立规范的煎药流程，将使中药汤剂的药效得到最大程度上的发挥。

Abstract

Objective:

To evaluate the effect of manual decocting and machine decocting on the chemical constituents in Bazhentang based on non-targeted metabolomics

and to find the differential chemical constituents of these two decocting methods.

Method:

Bazhentang was boiled by standardized manual decocting and machine decocting methods

respectively. Orthogonal partial least square-discriminate analysis(OPLS-DA) and other multivariate statistical methods

combined with variable importance in the projection(VIP) value and

-test

were employed to analyze the effect of two decocting methods on the chemical constituents in Bazhentang. The differential chemical constituents were analyzed by UPLC-LTQ-Orbitrap MS under positive and negative ion modes

mobile phase was acetonitrile-0.1%formic acid aqueous solution for gradient elution

the scanning range was

50-1 500.

Result:

Under the positive and negative ion modes of high-resolution mass spectrometry

a total of 87 differential components were found

40 of them were identified according to the mass spectrometry data and literature reports

including senkyunolide A

glycyrrhizin

ferulic acid

etc.

Conclusion:

Based on the analysis of color and chemical compositions of Bazhentang

there are obvious differences between the standardized manual decocting and machine decocting. If the advantages of these two methods are combined

a standardized decoction process can be established on the basis of maintaining the advantages of manual decocting

the effectiveness of traditional Chinese medicine decoction will be maximized and it will be convenient for patients to take it.

关键词

Keywords

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