Protective Effect of Phenolic Compound of Osmundae Rhizoma on Systemic Inflammatory Response Syndrome in Mice

Kai LI; Qing YANG; Tian-tian ZHOU; Si-chao YAN; Xiao-gang WENG; Lan YANG; Ying CHEN; Ya-jie WANG; Xiao-hui JIANG; Yuan GUO; Xiao-xin ZHU; Yu-jie LI

doi:10.13422/j.cnki.syfjx.20190909

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Protective Effect of Phenolic Compound of Osmundae Rhizoma on Systemic Inflammatory Response Syndrome in Mice

Pharmacology | 更新时间：2021-02-09

- Protective Effect of Phenolic Compound of Osmundae Rhizoma on Systemic Inflammatory Response Syndrome in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 9, Pages: 55-60(2019)
- 作者机构：
  
  1.安徽中医药大学，合肥　 230038
  2.中国中医科学院　青蒿素研究中心，北京　 100700
  3.中国中医科学院　中药研究所，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190909
  CLC： R2-0; R22; R285.5
- Received：20 December 2018，
  
  Published：05 May 2019
- 稿件说明：
移动端阅览
Kai LI, Qing YANG, Tian-tian ZHOU, et al. Protective Effect of Phenolic Compound of Osmundae Rhizoma on Systemic Inflammatory Response Syndrome in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 55-60.
DOI：

Kai LI, Qing YANG, Tian-tian ZHOU, et al. Protective Effect of Phenolic Compound of Osmundae Rhizoma on Systemic Inflammatory Response Syndrome in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 55-60. DOI： 10.13422/j.cnki.syfjx.20190909.

摘要

目的：

建立小鼠全身炎症反应综合征(SIRS)模型，探索紫萁贯众醇提取物中单体成分对羟基苄叉丙酮(4-hydroxybenzylideneacetone

HBAc)，3，4-二羟基苄叉丙酮(3，4-dihydroxybenzylideneacetone

DHBAc)对SIRS模型小鼠的保护作用及机制。

方法：

BALB/c小鼠随机分为正常组，模型组，HBAc

DHBAc低、中、高剂量(25，50，100 μg·kg

－1

)组。预防给药7 d后腹腔注射脂多糖(LPS)，造模5 h后检测小鼠肛温、呼吸频率、白细胞、血小板计数、白细胞分类、糖脂代谢以及肺组织炎症因子和炎症相关蛋白磷酸化情况。

结果：

与正常组比较，模型组小鼠腹腔注射LPS(6 mg·kg

－1

)可致小鼠呼吸频率降低(

0.05)，体温明显降低(

0.01)，外周血白细胞数和单核细胞百分比增加(

0.01)，血小板减少(

0.01)，血糖水平降低(

0.05)，肺组织中白细胞介素-1

分泌增多(

0.01)。与模型组比较，HBAc

DHBAc均明显增加动物呼吸频率，升高动物体温，降低外周白细胞水平以及单核细胞百分比(

0.05，

0.01)，并显著升高血糖水平(

0.05，

0.01)，减少肺组织中白细胞介素-1

的分泌(

0.01)。

结论：

腹腔注射LPS致小鼠SIRS模型成立，HBAc

DHBAc对LPS致小鼠SIRS模型有一定的保护作用，可能通过I

c-JUN通路发挥抗炎作用。

Abstract

Objective:

To investigate the protective effect of phenolic compounds 4-hydroxybenzylideneacetone and(HBAc)3

4-dihydroxybenzylideneacetone (DHBAc) of Osmundae Rhizoma on the systemic inflammatory response syndrome(SIRS) in mice by establishing the mice model of SIRS.

Method:

BALB/c mice were randomly divided into the normal group

the SIRS model group and the different doses of HBAC and DHBAc group (25

100 μg·kg

-1

). Lipopolysaccharides (LPS) was injected intraperitoneally after 7 days of prophylactic administration. After 5 hours of modeling

the anus temperature

respiratory rate

the number of white blood cell(WBC) and platelets(PLT)

WBC classification

glycolipid metabolism

inflammatory factor and signal transducing phosphorylated protein of lung were measured.

Result:

Intraperitoneal injection with LPS (6 mg·kg

-1

) in mice can significantly reduce the respiratory rate (

0.05) and the body temperature (

0.01)

decrease the number of WBC

PLT (

0.01

0.01) and blood glucose (

0.05)

and increase the secretion of interleukin-1

(

0.01). HBAC and DHBAC significantly increased the respiratory rate (

0.05

0.01) and the body temperature (

0.05

0.01) of mice

decreased MONO (

0.05

0.01)

and significantly increased the level of blood glucose (

0.05

0.01)

and decreased the secretion of interleukin-1

(

0.01).

Conclusion:

The SIRS model can be established through intraperitoneal injection of LPS. HBAc and DHBAc have protective effects on endotoxin-induced SIRS in mice

and may exert anti-inflammatory effects through IκB and c-JUN pathways.

关键词

Keywords

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