Objective:

2

Stems

petioles

stem sections with axillary and leaves of

Scrophularia ningpoensis

were taken as the material

in vitro

to screen out the suitable plantlet regeneration system and optimal exercising seedling conditions.

Method:

2

Different explants

hormones and concentrations on the induction and proliferation of cluster bud were studied by L

16

(4

5

) orthogonal test. One factor analysis of variance (ANOVA) was made on the induction of adventitious buds rooted with different concentrations of hormones. At the same time

different substrates

watering cycles and transition modes were selected to optimize key technologies of exercising seedlings of

S

.

ningpoensis

.

Result:

2

Stem sections with axillary was the best explant

which was followed by stems

leaves and petioles. The suitable media for the induction of adventitious buds was MS+ 6-BA 0.5 mg·L

-1

+ NAA 0.2 mg·L

-1

with the induction rate of 100.0% and the proliferation multiple of 9.84.The suitable media for root induction was 1/2 MS+ IBA 0.2 mg·L

-1

with the rooting rate of 100.0% and the number of roots of 39.45.For matrix

they were transplanted with nutrient soil

vermiculite and perlite (5∶2∶1) as the media

to keep proper matching of fertility

permeability and water retention. The container seedlings can grow well

and the survival rate was more than 95% when they were watered every 2 days

the acclimatization of plantlets took 20 days indoor and 10 days in shaded greenhouses.

Conclusion:

2

The clonal propagation system of

S

.

ningpoensis

was established to provide an effective way for the efficient

rapid and steady plantlet regeneration

the breeding of high-quality seedlings and the suitable exercising seedling conditions of

S

.

ningpoensis

.