Effect and Mechanism of Jiaotaiwan on Cognitive Impairment in Diabetic Mice

Juan-e LI; Xiao-fan JIANG

doi:10.13422/j.cnki.syfjx.20190921

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Effect and Mechanism of Jiaotaiwan on Cognitive Impairment in Diabetic Mice

Pharmacology | 更新时间：2021-02-09

- Effect and Mechanism of Jiaotaiwan on Cognitive Impairment in Diabetic Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 17, Pages: 23-27(2019)
- 作者机构：
  
  陕西省人民医院，西安交通大学　第三附属医院，西安　 710068
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190921
  CLC： R22; R242; R2-031; R285.5
- Received：11 October 2018，
  
  Published Online：17 January 2019，
  
  Published：05 September 2019
- 稿件说明：
移动端阅览
Juan-e LI, Xiao-fan JIANG. Effect and Mechanism of Jiaotaiwan on Cognitive Impairment in Diabetic Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(17): 23-27.
DOI：

Juan-e LI, Xiao-fan JIANG. Effect and Mechanism of Jiaotaiwan on Cognitive Impairment in Diabetic Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(17): 23-27. DOI： 10.13422/j.cnki.syfjx.20190921.

摘要

目的：

观察交泰丸(Jiaotaiwan，JTW)不同配伍比例对糖尿病胰岛素抵抗、认知功能障碍的作用，并探讨其机制。

方法：

自发性糖尿病db/db小鼠40只，随机分为模型组，交泰丸1号组(1.68 g·kg

－1

)，交泰丸2号组(3.36 g·kg

－1

)，交泰丸3号组(8.40 g·kg

－1

)，每组8只，另设同周龄C57BL/6J小鼠8只为正常组。干预8周后，检测小鼠学习记忆，空间探索能力；检测空腹血糖(fasting blood glucose，FBG)，胰岛素水平(fasting insulin，Fins)，计算胰岛素抵抗指数(HOMA-IR)，总甘油三酯(total triglyceride，TG)，总胆固醇(total cholesterol，TC)，游离脂肪酸(free fatty acids，FFA)，高密度脂蛋白(high density lipoprotein，HDL)，低密度脂蛋白(low density lipoprotein，LDL)等；蛋白免疫印迹法(Western blot)检测tau蛋白pSer199，pSer202，pSer214位点，磷酸化蛋白激酶B(p-Akt)，糖原合酶激酶-3

(glycogen synthase kinase-3

，GSK-3

)，磷酸化GSK-3

(p-GSK-3

)蛋白表达。

结果：

与模型组比较，交泰丸2，3号组FBG，Fins和HOMA-IR显著降低(

0.01)；TG，TC，HDL，FFA显著降低(

0.01)；分辨指数、穿越平台次数显著提高(

0.01)；小鼠海马tau蛋白pSer199，pSer202，pSer214位点，GSK-3

蛋白表达明显降低(

0.05，

0.01)，p-Akt，p-GSK-3

表达显著升高(

0.01)。

结论：

交泰丸3号(黄连-肉桂 10∶1)具有改善胰岛素抵抗及认知功能障碍的作用，可能与其抑制tau蛋白过度磷酸化，下调GSK-3

蛋白表达和上调p-Akt，p-GSK-3

表达有关。

Abstract

Objective:

To explore effect and mechanism of Jiaotaiwan (JTW) on cognitive impairment in diabetic mice.

Method:

The 40 db/db mice of spontaneous diabetes were randomly divided into model group

JTW 1 group (1.68 g·kg

-1

)

JTW 2 group (3.36 g·kg

-1

)

and JTW 3 group (8.40 g·kg

-1

)

with 8 mice in each group

and 8 C57BL/6J mice were included into normal group. After 8 weeks of treatment

behavioral test

fasting blood glucose (FBG)

fasting insulin (Fins)

insulin resistance index (HOMA-IR)

total triglyceride (TG)

total cholesterol (TC)

free fatty acids (FFA)

high density lipoprotein (HDL)

and low density lipoprotein (LDL) were detected. Western blot was used to detect protein expressions of pSer199

pSer202

pSer214

p-Akt

glycogen synthase kinase-3

(GSK-3

)

phospho-GSK-3

(p-GSK-3

Result:

Compared with model group

FBG

Fins

HOMA-IR

HDL

FFA in JTW group decreased significantly (

0.01)

with a significant improvement in cognitive function (

0.01)

protein expressions of taupSer199

pSer202

pSer214 and GSK-3

in hippocampus of mice decreased significantly (

0.05

0.01)

and expressions of p-Akt and p-GSK3 beta significantly increased (

0.01).

Conclusion:

JTW 3 (Coptidis Rhizoma-Cinnamomi Cortex 10∶1) can alleviate insulin resistance and cognitive impairment. The mechanism may be related to the inhibition of over-phosphorylation of tau protein

the down-regulation of GSK-3

protein expression and the up-regulation of p-Akt and p-GSK-3

expressions.

关键词

Keywords

references

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