Effect of Erzhiwan on Invasion and Metastasis of Human Colon Cancer Cells

Hua-ying WANG; Wen-yan YU; Guo-juan WANG; Hong-ning LIU; Xiang-yan WEI; Peng-yun YING

doi:10.13422/j.cnki.syfjx.20190922

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Effect of Erzhiwan on Invasion and Metastasis of Human Colon Cancer Cells

Pharmacology | 更新时间：2021-02-09

- Effect of Erzhiwan on Invasion and Metastasis of Human Colon Cancer Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 17, Pages: 28-33(2019)
- 作者机构：
  
  1.江西中医药大学　中医基础理论分化发展研究中心，江西省中医病因生物学重点实验室，南昌　 330004；
  2.江西中医药大学　附属医院，南昌　 330006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190922
  CLC： R22; R242; R2-031; R285.5
- Received：14 October 2018，
  
  Published Online：17 January 2019，
  
  Published：05 September 2019
- 稿件说明：
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Hua-ying WANG, Wen-yan YU, Guo-juan WANG, et al. Effect of Erzhiwan on Invasion and Metastasis of Human Colon Cancer Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(17): 28-33.
DOI：

Hua-ying WANG, Wen-yan YU, Guo-juan WANG, et al. Effect of Erzhiwan on Invasion and Metastasis of Human Colon Cancer Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(17): 28-33. DOI： 10.13422/j.cnki.syfjx.20190922.

摘要

目的：

探讨二至丸(Erzhiwan，EZW)对人属结肠癌细胞株HCT116侵袭及迁移的作用。

方法：

以人结肠癌细胞株HCT116为研究对象，以化疗药奥沙利铂(oxaliplatin

L-OHP)作为阳性对照，以20%，10%，5% EZW含药血清做为高、中、低剂量，运用Transwell法检测EZW及联用L-OHP后对HCT116细胞侵袭的影响，划痕实验观察细胞迁移情况，蛋白免疫印迹法(Western blot，WB)检测其对细胞血管内皮生长因子(vascular endothelial growth factor，VEGF)，E-钙黏蛋白(E-cadherin)表达的影响。

结果：

①Transwell实验显示，与空白组比较，各用药组穿模细胞数均减少(

0.05)，以联用后穿模细胞数最少。②划痕实验显示，与空白组比较，各用药组均能抑制HCT116细胞的迁移(

0.05)。12 h EZW高浓度组抑制率高于L-OHP组(

0.05)，其余时间段EZW各剂量组抑制率低于L-OHP组(

0.05)，各时间段联用组抑制率均高于EZW高、中、低剂量组(

0.05)，12 h联用组抑制率高于L-OHP组(

0.05)，48 h联用组抑制率低于L-OHP组(

0.05)。③WB实验显示，与空白组比较，10%，20%EZW组，VEGF蛋白表达量均明显降低(

0.05)，联用组与L-OHP组相比蛋白表达量降低(

0.05)；与空白组相比，EZW含药血清各组降低了E-cadherin蛋白的表达(

0.05)，联用组和L-OHP组E-cadherin蛋白表达量均显著升高，且联用后E-cadherin蛋白表达量最高(

0.05)。

结论：

二至丸能抑制结肠癌HCT116细胞侵袭转移，并以协同L-OHP后效果最佳，可能与其降低 VEGF 蛋白表达，协同L-OHP升高 E-cadherin 蛋白表达有关。

Abstract

Objective:

To investigate the effect of Erzhiwan (EZW) on the invasion and metastasis of human colon cancer HCT116 cells.

Method:

The abilities of invasion (number of transmembrane cells) and migration (relative width of 48 h scratch) were observed by Transwell assay. Western blot was used to detect vascular endothelial growth factor (VEGF) and E-cadherin protein

respectively.

Result:

① Transwell results showed that compared with the blank control group

the number of model piercing cells in each drug group was decreased (

0.05)

and the number of perforating model cells was the least after combined use of L-OHP. ②Scratch test results showed that compared with the blank control group

the migration of HCT116 cells was inhibited in each treatment group (

0.05). The inhibitory rate of high-dose EZW group was higher than that of L-OHP group at 12 h (

0.05)

and the inhibition rate of EZW in other time groups was lower than that of L-OHP group (

0.05). The inhibition rate of combined treatment group was higher than that of EZW groups (

0.05). The figure of combined group was higher than that of L-OHP group at 12 h (

0.05)

and combined group was lower than L-OHP group at 48 h (

0.05). ③ Western blot results showed that compared with the control group

the expressions of VEGF protein in 10% and 20%EZW group were significantly decreased (

0.05)

and the expression of VEGF protein in 5.19 mg·L

-1

L-OHP combined with 10% EZW group was lower than that in L-OHP group (

0.05). Compared with the blank control group

EZW groups decreased the expression of E-cadherin protein (

0.05). And 5.19 mg·L

-1

L-OHP combined with 10% EZW group and L-OHP group increased the expression of E-cadherin protein significantly. Compared with L-OHP group

the expression of E-cadherin protein was the highest (

0.05).

Conclusion:

EZW can inhibit the invasion and metastasis of colon cancer HCT116 cells

and the best effect is achieved after combined with L-OHP. This may be related to the decrease of VEGF protein expression and the increase of E-cadherin protein expression after combination with L-OHP.

关键词

Keywords

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