Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis

Dong-sheng WU; Hui CAO; Yu ZHANG; Ya GAO; Dong-hua BIN

doi:10.13422/j.cnki.syfjx.20190936

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Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis

Classic Prescriptions | 更新时间：2021-02-09

- Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 9, Pages: 6-11(2019)
- 作者机构：
  
  1.湖南中医药大学，长沙　 410208
  2.湖南中医药大学　第一附属医院，长沙　 410007
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190936
  CLC： R289; R318.14; R516.1; R364.7; R285.5
- Received：03 November 2018，
  
  Published Online：18 January 2019，
  
  Published：05 May 2019
- 稿件说明：
移动端阅览
Dong-sheng WU, Hui CAO, Yu ZHANG, et al. Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 6-11.
DOI：

Dong-sheng WU, Hui CAO, Yu ZHANG, et al. Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 6-11. DOI： 10.13422/j.cnki.syfjx.20190936.

摘要

目的：

探讨芍药汤对2，4，6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎大鼠肠黏膜免疫屏障的影响。

方法：

将60只SD大鼠随机分为正常组，模型组，美沙拉嗪组(0.067 g·kg

－1

)，芍药汤低、中、高剂量组(1.8，3.6，7.2 g·kg

－1

)，采用TNBS诱导溃疡性结肠炎模型，对应给予生理盐水、美沙拉嗪、芍药汤灌胃治疗7 d，采用苏木素-伊红(HE)染色观察结肠组织病理学改变，免疫组化法和蛋白免疫印迹法(Western blot)检测肠黏膜中CD4

＋

T淋巴细胞数量和分泌型免疫球蛋白A(SIgA)蛋白的表达。

结果：

与正常组比较，模型组大鼠肠黏膜损伤程度评分及病理学评分显著升高(

0.01)；CD4

＋

T淋巴细胞，SIgA蛋白表达显著下降(

0.01)；与模型组比较，各给药组大鼠结肠黏膜损伤程度评分及病理学评分显著下降(

0.01)，肠黏膜CD4

＋

T淋巴细胞，SIgA表达明显升高(

0.05，

0.01)；与美沙拉嗪组及芍药汤低剂量组比较，芍药汤中、高剂量组肠黏膜损伤程度评分和病理学评分显著下降(

0.01)，芍药汤中、高剂量组大鼠肠黏膜中CD4

＋

T淋巴细胞及SIgA蛋白表达明显升高(

0.05)。

结论：

芍药汤可通过增加肠黏膜中CD4

＋

T细胞数量及SIgA分泌的表达，起到减轻肠黏膜损伤，保护肠黏膜免疫屏障功能作用。

Abstract

Objective:

To investigate effect of Shaoyaotang on intestinal mucosal immune barrier induced by 2

6-trinitrobenzene sulphonic acid (TNBS) in rats with ulcerative colitis.

Method:

Sixty SD rats were randomly divided into normal group

model group

mesalazine group (0.067 mg·kg

-1

)

low

medium and high-dose Shaoyaotang groups (1.8

3.6

7.2 g·kg

-1

). In the TNBS-induced ulcerative colitis model

saline

mesalazine

peony soup were administered by gavage for 7 days. Hematoxylin-eosin(HE) staining was used to detect the histopathological changes of colon tissue. The number of CD4

T lymphocytes and the expression of secretory immunoglobulin A(SIgA) in intestinal mucosa were detected by immunohistochemistry and Western blot.

Result:

Compared with normal group

the scores of intestinal mucosal injury and the pathological scores in model group increased significantly (

0.01). The expressions of CD4

T lymphocytes and SIgA in the intestinal mucosa of model group decreased significantly (

0.01). Compared with model group

the scores of colonic mucosal damage and the pathological scores decreased significantly in rats (

0.01). The expressions of CD4

T lymphocytes and SIgA in the intestinal mucosa of rats in each group elevated significantly (

0.05

0.01). Compared with mesalazine group and low-dose Shaoyaotang group

the intestinal mucosal injury score and the pathological scores in medium and high-dose Shaoyaotang groups decreased significantly (

0.01). The expression levels of CD4

T lymphocytes and SIgA protein in the intestinal mucosa of rats in middle and high doses Shaoyaotang groups increased significantly (

0.05).

Conclusion:

Shaoyaotang can reduce the intestinal mucosal damage and protect the intestinal mucosal immune barrier by increasing the number of CD4

T cells and the expression of SIgA secretion in the intestinal mucosa.

关键词

Keywords

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