Effect of Duhuo Jisheng Tang on PERK/Bip Signaling Pathway in Knee Osteoarthritis Model Rats

Lin-can ZHAO; Wei-wei LI; Yi-ming WU; Wei-feng DUAN

doi:10.13422/j.cnki.syfjx.20190937

您当前的位置：

首页 >

文章列表页 >

Effect of Duhuo Jisheng Tang on PERK/Bip Signaling Pathway in Knee Osteoarthritis Model Rats

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Duhuo Jisheng Tang on PERK/Bip Signaling Pathway in Knee Osteoarthritis Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 9, Pages: 18-24(2019)
- 作者机构：
  
  1.郑州澍青医学高等专科学校，郑州　 450000
  2.河南中医药大学　第三附属医院，郑州　 450004
  3.河南省洛阳正骨医院，河南　洛阳　 471000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190937
  CLC： R289; R684.3; R274; R285.5
- Received：02 November 2018，
  
  Published Online：22 January 2019，
  
  Published：05 May 2019
- 稿件说明：
移动端阅览
Lin-can ZHAO, Wei-wei LI, Yi-ming WU, et al. Effect of Duhuo Jisheng Tang on PERK/Bip Signaling Pathway in Knee Osteoarthritis Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 18-24.
DOI：

Lin-can ZHAO, Wei-wei LI, Yi-ming WU, et al. Effect of Duhuo Jisheng Tang on PERK/Bip Signaling Pathway in Knee Osteoarthritis Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 18-24. DOI： 10.13422/j.cnki.syfjx.20190937.

摘要

目的：

基于蛋白激酶R样内质网激酶(PERK)／免疫球蛋白结合蛋白(Bip)信号通路探讨独活寄生汤缓解膝骨关节炎的可能机制。

方法：

采用寒冷刺激法建立膝骨性关节炎模型，将大鼠随机分为空白组，模型组，塞来昔布组(0.021 g·kg

－1

)和独活寄生汤低、中、高剂量组(8.37，16.72，33.48 g·kg

－1

)，空白组和模型组给予等体积的生理盐水，记录各组大鼠膝关节直径变化。采用苏木素-伊红(HE)染色观察大鼠膝关节软骨病理形态变化；酶联免疫吸附测定(ELISA)检测血清中肿瘤坏死因子-

(TNF-

)，白细胞介素-1

(IL-1

)，透明质酸(HA)的表达；采用实时荧光定量PCR(Real-time PCR)和蛋白免疫印迹法(Western bolt)检测软骨中PERK

Bip，半胱氨酸天冬氨酸蛋白酶-9(Caspase-9)mRNA和蛋白的表达。

结果：

与模型组比较，塞来昔布组和独活寄生汤高剂量组大鼠膝关节红肿明显改善，且关节直径均显著的减小(

0.01)，塞来昔布组和独活寄生汤高剂量组大鼠膝关节软骨局部裂隙缺损有明显好转。与空白组比较，模型组大鼠TNF-

，IL-1

和HA表达水平显著升高(

0.01)，大鼠软骨中PERK

Bip

Caspase-9的mRNA和蛋白表达水平明显升高(

0.05，

0.01)；与模型组比较，塞来昔布组和独活寄生汤高剂量组大鼠血清中TNF-

，IL-1

和HA表达明显下降(

0.05，

0.01)，塞来昔布组和独活寄生汤高剂量组大鼠软骨中PERK

Bip

Caspase-9的mRNA和蛋白表达水平明显降低(

0.05，

0.01)。

结论：

独活寄生汤能缓解膝骨关节炎模型大鼠的症状，其机制可能与调节大鼠软骨中PERK/Bip信号通路有关。

Abstract

Objective:

To explore the possible mechanism of Duhuo Jisheng Tang in relieving knee osteoarthritis based on protein kinase R-like endoplasmic reticulum kinase(PERK)/immunoglobulin-binding protein(Bip)signaling pathway.

Method:

A model of knee osteoarthritis was established by cold stimulation.Rats were randomly divided into blank group

model group

celecoxib group(0.021 g·kg

-1

)

low

medium and high-dose Duhuo Jisheng Tang groups (8.37

16.72

33.48 g·kg

-1

). Blank group and model group were given equal volume of physiological saline.The changes of knee joint diameter were recorded.The pathological changes of rat articular cartilage were observed by hematoxylin-eosin (HE) staining.The expressions of tumor necrosis factor-alpha(TNF-

)

interleukin-1

(IL-1

)and hyaluronic acid(HA)in serum were detected by enzyme-linked immunosorbent assay(ELISA). The mRNA and protein expression levels of PERK

Bip and cysteinyl as parates pecific protein-9(Caspase-9)in cartilage were detected by Real-time PCR and Western blot.

Result:

The knee joint redn ess and the joint diameter of celecoxib group and high-dose Duhuo Jisheng Tang group were improved

and the joint diameter was reduced significantly(

0.01). The local fractures of knee joint cartilage in the celecoxib group and the high-dose Duhuo Jisheng Tang group were significantly improved.Compared with blank group

the expression levels of TNF-

IL-1

and HA were increased in model group(

0.01). The gene and protein expression levels of PERK

Bip and Caspase-9 in cartilage of the model group were increased(

0.05

0.01). Compared with model group

the expressions of TNF-

IL-1

and HA in serum of celecoxib group and high-dose Duhuo Jisheng Tang group were decreased(

0.05

0.01). The mRNA and protein expression levels of PERK

Bip and Caspase-9 in cartilage of celecoxib group and high-dose Duhuo Jisheng Tang group were decreased(

0.05

0.01).

Conclusion:

Duhuo Jisheng Tang can alleviate the symptoms of knee osteoarthritis model rats

and its mechanism may be related to the regulation of PERK/Bip signaling pathway in rat cartilage.

关键词

Keywords

references

王文燕，庞金辉，石继祥，等．骨关节炎炎症的研究进展 [J]. 世界最新医学信息文摘 2018 , 18 ( 15 ): 34 - 35 .

薛浩，刘岩，冉博，等．膝关节骨性关节炎治疗现状 [J]. 医学综述， 2018 , 24 ( 2 ): 321 - 325,330 .

俞颖豪，赵继军，艾山尔吐，等．阿合奇县柯尔克孜族膝关节骨关节炎流行病学调查 [J]. 实用骨科杂志， 2018 , 24 ( 3 ): 226 - 229 .

陈峰，石晓兵．膝骨性关节炎临床分期的现状和研究进展 [J]. 广西医科大学学报， 2018 , 35 ( 3 ): 412 - 415 .

陈加荣，李凭跃．膝关节骨性关节炎的阶梯治疗原则 [J]. 中国骨科临床与基础研究杂志， 2018 , 10 ( 1 ): 53 - 57 .

田惠萍，刘莹，王海洋，等．膝骨关节炎的中医药治疗临床研究进展 [J]. 风湿病与关节炎， 2018 , 7 ( 1 ): 67 - 71 .

WANG F M , Galson D L , Roodman G D , et al . Resveratrol triggers the pro-apoptotic endoplasmic reticulum stress response and represses pro-survival XBP1 signaling in human multiple myeloma cells [J]. Exp Hematol , 2011 , 39 ( 10 ): 999 - 1006 .

陈俊，吴广文，许惠凤，等．独活寄生汤干预膝骨关节炎模型大鼠软骨PERK/Bip信号通路的表达 [J]. 中国组织工程研究， 2018 , 22 ( 28 ): 4493 - 4500 .

郑自然，唐仕欢．骨质疏松症与骨性关节炎中医治疗方剂用药规律比较分析 [J]. 中国中药杂志， 2014 , 39 ( 16 ): 3172 - 3175 .

马拥军．独活寄生汤治疗膝关节骨性关节炎32例临床观察 [J]. 中医学报， 2018 , 33 ( 1 ): 153 - 157 .

傅品来，丘青中，李剑．独活寄生汤联合塞来昔布胶囊防治早期膝骨性关节炎临床研究 [J]. 世界中西医结合杂志， 2018 , 13 ( 1 ): 66 - 69 .

夏璇，黄清春，何羿婷．独活寄生汤治疗膝骨关节炎临床疗效Meta分析 [J]. 新中医， 2018 , 50 ( 5 ): 208 - 211 .

谢鸣．方剂学 [M]．北京：人民卫生出版社， 2010 ： 271 .

李翠葳．从Wnt/β-catenin通路探讨益气养血方干预膝骨关节炎大鼠软骨退变的机制研究 [D]．昆明：云南中医学院， 2017 .

叶大林，谢平金，魏合伟，等．补肾活血方调控膝骨性关节炎大鼠软骨下骨的作用机制研究 [J]. 中国骨质疏松杂志， 2018 , 24 ( 12 ): 1606 - 1611 .

冯小波，朱思刚．独活寄生汤对髌骨软化症髌骨软骨及膝关节液中IL-1，MMP-1，GAG的影响 [J]. 中国实验方剂学杂志， 2015 , 21 ( 17 ): 156 - 160 .

程志安，胡广兵，刘幸明，等．独活寄生汤治疗老年性腰膝疼痛临证体悟 [J]. 新中医， 2018 , 50 ( 2 ): 175 - 177 .

高尚轸，张小宁．独活寄生汤组方治疗慢性腰痛思路探析 [J]. 亚太传统医药， 2014 , 10 ( 12 ): 53 - 54 .

麦海燕，黄小舟，徐能．独活寄生汤缓解膝关节骨性关节炎疼痛的疗效分析 [J]. 中国当代医药， 2013 , 20 ( 19 ): 97 - 98 .

张君涛，吴超超，张栋林，等．膝关节骨性关节炎动物模型研究进展 [J]. 重庆医学， 2018 , 47 ( 15 ): 2061 - 2063,2067 .

宋寒冰，赵峰，葛圆圆，等．膝关节骨性关节炎动物模型建立的研究现状 [J]. 中国医药导报， 2017 , 14 ( 30 ): 46 - 49 .

张冰，王洋，王建民．膝关节骨性关节炎的诊断与治疗进展 [J]. 甘肃医药， 2018 , 37 ( 3 ): 204 - 208 .

WANG H J , YING A O , CHEN L X , et al . Second-look arthroscopic evaluation of the articular cartilage after primary single-bundle and double-bundle anterior cruciate ligament reconstructions [J]. Chinese Med J-Peking , 2011 , 124 ( 21 ): 3551 .

贾峻，荣兵，李建，等．独活寄生汤含药血清对膝骨性关节炎大鼠软骨细胞代谢，BMP-7及SIRT1表达的影响 [J]. 中国实验方剂学杂志， 2017 , 23 ( 17 ): 159 - 165 .

Herrman M , Anders S , Straub R H , et al . TNF inhibits catecholamine production from induced sympathetic neuron-like cells in rheumatoid arthritis and osteoarthritis in vitro [J]. Sci Rep , 2018 , 8 ( 1 ): 9645 - 9657 .

Vinatier C , Domínguez E , Guicheux J , et al . Role of the inflammation-autophagy-senescence integrative network in osteoarthritis [J]. Front Physiol , 2018 , 9 ( 41 ): 706 - 721 .

Bokhari R A , Naca T , Lau S F , et al . Java tea(orthosiphon stamineus)protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study [J]. Inflammopharmacology , 2018 , 26 ( 2 ): 1 - 11 .

WEI Y , JIA J , JIN X , et al . Resveratrol ameliorates inflammatory damage and protects against osteoarthritis in a rat model of osteoarthritis [J]. Mol Med Rep , 2018 , 17 ( 1 ): 1493 - 1498 .

Recnik G , Kralj-Iglič V , Iglič A , et al . Higher peak contact hip stress predetermines the side of hip involved in idiopathic osteoarthritis [J]. Clin Biomech , 2007 , 22 ( 10 ): 1119 - 1124 .

叶锦霞，吴广文，赖舜淼，等．透骨消痛胶囊对膝骨关节炎大鼠内质网应激影响的研究 [J]. 风湿病与关节炎， 2015 , 4 ( 11 ): 5 - 11 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Chinese Medicine in Treatment of Osteoarthritis by Regulating Tumor Necrosis Factor and Signaling Pathways： A Review

Invasion and Metastasis Inhibition of Lung Cancer by Traditional Chinese Medicine： A Review

Traditional Chinese Medicine Intervention in Signaling Pathways Related to Benign Prostatic Hyperplasia： A Review

Mechanism of Anmeidan in Improving Learning and Memory in Insomnia Model Rats by Mediating Immunoinflammation via cGAS/STING Signaling Pathway

Role of VEGF Signaling Pathway in Pathological Mechanism of Colorectal Cancer and Traditional Chinese Medicine Intervention： A Review

Related Author

JIA Ziyue

ZHANG Shaozhuo

ZHENG Fengyu

LI Jia

SUN Xu

ZHANG Lu

LIU Huaimin

LI Shenglong

Related Institution

School of Chinese Materia Medica，Tianjin University of Traditional Chinese Medicine

The Second Clinical Medical College， Henan University of Chinese Medicine

The Affiliated Cancer Hospital of Zhengzhou University/Henan Cancer Hospital

School of Traditional Chinese and Western Medicine，Gansu University of Chinese Medicine

School of Basic Medical Sciences，Gansu University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰