Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model

Yue CAI; Bo ZHANG; Jing GUO

doi:10.13422/j.cnki.syfjx.20191003

您当前的位置：

首页 >

文章列表页 >

Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 11, Pages: 29-33(2019)
- 作者机构：
  
  天津中医药大学　第一附属医院，天津　 300193
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191003
  CLC： R2-0; R22; R285.5; R289
- Received：01 October 2018，
  
  Published Online：12 February 2019，
  
  Published：05 June 2019
- 稿件说明：
移动端阅览
Yue CAI, Bo ZHANG, Jing GUO. Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 29-33.
DOI：

Yue CAI, Bo ZHANG, Jing GUO. Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 29-33. DOI： 10.13422/j.cnki.syfjx.20191003.

摘要

目的：

观察佐剂性关节炎(AA)小鼠滑膜组织成纤维样细胞(FLS)增殖的变化及甘草附子汤对其的影响。

方法：

将48只雄性Balb/c小鼠随机分为正常组，模型组，甘草附子汤组(7.8 g·kg

－1

)，雷公藤多苷组(10 mg·kg

－1

)。采用AA小鼠模型，模型成功后，治疗组小鼠给予相应给药灌胃18 d，1次/d，正常组和模型组小鼠灌胃等体积生理盐水。采用足肿胀法评价药物治疗作用；苏木素-伊红(HE)染色观察各组踝关节组织病理变化；Vimentin免疫组化法检测滑膜组织成纤维样增殖；免疫荧光法检测肿瘤坏死因子-

(TNF-

)表达水平以及蛋白免疫印迹法(Western blot)检测滑膜组织中细胞周期蛋白 D

(Cyclin D

)，增殖细胞核抗原(PCNA)，p53和p21蛋白表达。

结果：

与正常组比较，模型组足肿胀度增加，滑膜增生和骨侵蚀明显，滑膜组织成纤维样细胞增殖率升高(

0.05)，滑膜组织中TNF-

，Cyclin D

，PCNA蛋白表达明显升高(

0.05)，p53和p21蛋白表达降低(

0.05)。与模型组比较，甘草附子汤组小鼠足肿胀，滑膜增生和骨侵蚀有所改善，滑膜组织成纤维样细胞增殖率降低(

0.05)，滑膜组织中TNF-

水平，Cyclin D

，PCNA蛋白表达显著降低，而p53和p21蛋白表达升高(

0.05)。

结论：

甘草附子汤对AA小鼠关节和滑膜损伤具有明显改善作用，可能与降低局部慢性炎症，调节AA小鼠滑膜Cyclin D

，PCNA，p53和p21的蛋白异常表达，抑制FLS增殖有关。

Abstract

Objective:

To investigate the effect of Gancao Fuzitang on the proliferation of fibroblast-like synoviocytes (FLS) in synovial tissue of mice with adjuvant-induced arthritis (AA).

Method:

The 48 male Balb/c mice were randomly divided into 4 groups: normal control group

model group(arthritis induced through injection with adjuvant)

Gancao Fuzitang group and triptolide group. The normal control group and the model control group were orally given physiological saline. After the successful modeling

the mice of the other two groups were treated with Gancao Fuzitang or triptolide orally for 18 days. Then the animals were euthanized

the paw edema of the animals was measured

and arthritic ankles were observed by histological examination. Tumor necrosis factor (TNF)-

was examined by immunoassays. The proliferation of synovial cells of foot joints was detected by immunohistochemical expressions of Vimentin. The expressions of Cyclin D

proliferating cell nuclear antigen (PCNA)

p53 and p21 were detected by Western blot.

Result:

Compared with the normal control group

the mice of synovial hyperplasia and bone erosion in model group were obvious. The joint swelling degree

TNF-

level and proliferation of FLS were increased (

0.05). The expressions of Cyclin D

PCNA were up-regulated(

0.05)

but the expressions of p53 and p21 were down-regulated(

0.05). Compared with the model group

Gancao Fuzitang significantly alleviated joint swelling and injuries

reduced the level of TNF-

cell cycles (Cyclin D

and PCNA)

and increased protein expressions of p53 and p21 (

0.05).

Conclusion:

Gancao Fuzitang has a therapeutic effect on AA mice

and the mechanism might be associated with its anti-inflammatory effect and the effects in regulating Cyclin D

PCNA

p53 and p21 expressions and inhibiting FLS proliferation.

关键词

Keywords

references

熊江华，李艳．中药复方对类风湿性关节炎干预机制的研究进展 [J]．中国实验方剂学杂志， 2017 ， 23 ( 9 )： 230 - 234 ．

Tolboom T C , Pieterman E , van der Laan W H , et al . Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10 [J]. Ann Rheum Dis , 2002 , 61 : 975 - 980 .

刘佳维，王永辉，李艳彦，等．黄芪桂枝五物汤对CIA模型大鼠关节滑膜细胞凋亡的影响 [J]．中国实验方剂学杂志， 2017 ， 23 ( 14 )： 171 - 176 ．

ZONG M , LU T , FAN S , et al . Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis [J]. Arthritis Res Ther , 2015 , 14 ( 17 ): 100 .

冯亚兵．甘草附子汤治疗风湿病及对抗环瓜氨酸肽抗体和类风湿因子的影响研究 [J]．亚太传统医药， 2018 ， 14 ( 5 )： 191 - 192 ．

罗晓光，曾萍萍，闫兵，等．甘草附子汤治疗寒湿痹阻型急性痛风性关节炎的临床观察 [J]．光明中医， 2018 ， 33 ( 4 )： 528 - 530 ．

牛立新，何丽清．甘草附子汤和姜苓半夏汤合方治疗慢性痛风性关节炎40例临床观察 [J]．山西中医学院学报， 2016 ， 17 ( 4 )： 31 - 32 ．

沈彩红，刘云，余宪文，等．茜草醇提物对佐剂性关节炎大鼠肝脏、脾脏损伤及Foxp3的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 7 )： 147 - 153 ．

LI X F , SHEN W W , SUN Y Y , et al . MicroRNA-20a negatively regulates expression of NLRP3-inflammasome by targeting TXNIP in adjuvant-induced arthritis fibroblast-like synoviocytes [J]. Joint Bone Spine , 2016 , 83 ( 6 ): 695 - 700 .

许红娣．督灸结合甘草附子汤对强直性脊柱炎活动期炎症指标的影响 [J]．针灸临床杂志， 2017 ， 33 ( 10 )： 16 - 19 ．

蔡悦，梁红玉，王建立，等．甘草附子汤对佐剂性关节炎小鼠的治疗作用 [J]．中国实验方剂学杂志， 2018 ， 24 ( 14 )： 142 - 146 ．

Bartok B , Firestein G S . Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis [J]. Immunol Rev Immunol Rev , 2010 , 233 ( 1 ): 233 - 255 .

LAO M , SHI M , ZOU Y , et al . Protein inhibitor of activated STAT3 regulates migration, invasion, and activation of fibroblast-like synoviocytes in rheumatoid arthritis [J]. J Immunol , 2016 , 196 ( 2 ): 596 - 606 .

ZHANG T , LI H , SHI J , et al . p53 predominantly regulates IL-6 production and suppresses synovial inflammation in fibroblast-like synoviocytes and adjuvant-induced arthritis [J]. Arthritis Res Ther , 2016 , 18 ( 1 ): 271 .

马凰富，李海松，赵宗江，等．雷公藤多苷诱导生精障碍大鼠模型的建立 [J]．中华男科学杂志， 2015 ， 21 ( 2 )： 179 - 184 ．

黄顺，林清华，王祖庆，等．甘草附子汤对类风湿关节炎大鼠ASIC3和HIF-1α表达的影响 [J]．中医药导报， 2018 ， 24 ( 18 )： 11-14，24 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Inhibitory Effect of Gancao Fuzitang on Bone Destruction in Collagen-induced Arthritis Mice by Regulating NF-κB Signaling Pathway

Effect of Shexiang Wulong Pill on Pathomorphology and Vascular Endothelial Growth Factor, Endostatin of Synovium in Rats with Adjuvant Arthritis

Effect of Shexiang Wulong Wan on the Pathomorphology and Expresssion of TNF- and IL-6 in Synovium in the Rats with Adjuvant Arthritis

Rapeutic Effect of Gancao Fuzi Tang on Adjuvant Arthritis Mouse Model

Analysis of Mechanism of Astragaloside Ⅳ in Regulating Ferroptosis Through SLC7A11/GPX4 Pathway Against Vascular Smooth Muscle Cell Proliferation

Related Author

QIAN Kai

ZHENG Xuexia

LI Haihong

CHEN Chen

SHEN Xinfeng

LIAO Zhiyi

ZHU Yiping

XU Chuanming

Related Institution

Guangzhou University of Chinese Medicine

Southern Medical University

Hebei Key Laboratory of Traditional Chinese Medicine（TCM） Research on Cardiocerebrovascular Disease，TCM Processing Technology Innovation Center of Hebei Province，College of Pharmacy， Hebei University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Southern Medical University Hospital of Integrated Chinese and Western Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰