Effect of Different Hormone Ratios on Callus Induction and Flavone Accumulation in Polygala tenuifolia

Jie LI; Ben-xiang HU; Liang PENG; Bing-yue YANG; Lu LUO; Fu-lin CAO; Ting ZHAO; Yan-ru AN; Tao HUANG

doi:10.13422/j.cnki.syfjx.20191112

您当前的位置：

首页 >

文章列表页 >

Effect of Different Hormone Ratios on Callus Induction and Flavone Accumulation in Polygala tenuifolia

Resource and Quality Evaluation | 更新时间：2021-02-09

- Effect of Different Hormone Ratios on Callus Induction and Flavone Accumulation in Polygala tenuifolia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 19, Pages: 131-137(2019)
- 作者机构：
  
  陕西中医药大学　药学院，陕西　咸阳　 712046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191112
  CLC： R284.1; R289; R22; R2-031
- Received：17 August 2018，
  
  Published Online：21 February 2019，
  
  Published：05 October 2019
- 稿件说明：
移动端阅览
Jie LI, Ben-xiang HU, Liang PENG, et al. Effect of Different Hormone Ratios on Callus Induction and Flavone Accumulation in Polygala tenuifolia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 131-137.
DOI：

Jie LI, Ben-xiang HU, Liang PENG, et al. Effect of Different Hormone Ratios on Callus Induction and Flavone Accumulation in Polygala tenuifolia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 131-137. DOI： 10.13422/j.cnki.syfjx.20191112.

摘要

目的：

研究不同激素配比对远志根、茎、叶愈伤组织诱导的影响，并对远志根、茎、叶愈伤组织中的黄酮量进行测定分析。

方法：

以MS为基本培养基，分别以远志无菌苗的根、茎、叶为外植体，应用正交试验法确定2，4-二氯苯氧乙酸(2，4-D)，萘乙酸(NAA)，6-苄氨基嘌呤(6-BA)这3种激素对远志根、茎、叶不同部位愈伤组织诱导及其黄酮积累量的影响。

结果：

2，4-D，NAA，6-BA对远志根、茎、叶愈伤诱导率均有显著影响。叶的最佳愈伤诱导组合为MS＋3.0 mg·L

－1

2，4-D＋1.0 mg·L

－1

NAA＋1.5 mg·L

－1

6-BA；茎的最佳愈伤诱导组合为MS＋1.0 mg·L

－1

2，4-D＋3.0 mg·L

－1

NAA＋1.5 mg·L

－1

6-BA；根的最佳愈伤诱导组合为MS＋1.0 mg·L

－1

2，4-D＋1.0 mg·L

－1

NAA＋1.0 mg·L

－1

6-BA。2，4-D，NAA，6-BA对远志茎愈伤组织中黄酮积累均影响显著，以MS＋3.0 mg·L

－1

2，4-D＋1.0 mg·L

－1

NAA＋0.5 mg·L

－1

6-BA为最佳黄酮积累组合；NAA，6-BA对远志叶愈伤组织中黄酮积累影响均显著，而2，4-D则无明显影响，以MS＋3.0 mg·L

－1

2，4-D＋2.0 mg·L

－1

NAA＋1.0 mg·L

－1

6-BA为最佳黄酮积累组合；3种激素分别对远志根愈伤组织中黄酮积累无明显影响，以MS＋2.0 mg·L

－1

2，4-D＋1.0 mg·L

－1

NAA＋1.5 mg·L

－1

6-BA为最佳黄酮积累组合。

结论：

在该条件下远志根、茎、叶愈伤组织诱导率均达100%，其中尤以远志叶愈伤组织长势最好，其次分别是远志茎和远志根。在该条件下远志根、茎、叶愈伤组织中黄酮量分别达到21.31，24.56，23.61 mg·g

－1

。

Abstract

Objective:

To study the effect of different hormone ratios on the callus induction of roots

stems and leaves of

Polygala tenuifolia

and determine and analyze the amount of flavonoids in roots

stems and leaves of

tenuifolia

Method:

With MS as the basic medium and roots

stems and leaves of

tenuifolia

sterile seedings as explants

the effects of 2

4-D

NAA and 6-BA on callus induction and flavonoid accumulation in different parts of roots

stems and leaves of

tenuifolia

were determined by orthogonal test.

Result:

4-D

NAA and 6-BA had significant effects on the callus induction rate of roots

stems and leaves of

tenuifolia

. The optimal callus induction combination of leaves was MS+ 3.0 mg·L

-1

4-D+ 1.0 mg·L

-1

NAA+ 1.5 mg·L

-1

6-BA

the optimal callus induction combination of stems was MS+ 1.0 mg·L

-1

4-D+ 3.0 mg·L

-1

NAA+ 1.5 mg·L

-1

6-BA

the optimal callus induction combination for roots was MS+ 1.0 mg·L

-1

4-D+ 1.0 mg·L

-1

NAA+ 1.0 mg·L

-1

6-BA. And 2

4-D

NAA and 6-BA had significant effects on flavonoid accumulation in the stem callus of

tenuifolia

and MS+ 3.0 mg·L

-1

4-D+ 1.0 mg·L

-1

NAA+ 0.5 mg·L

-1

6-BA was the best flavonoid accumulation combination.NAA

6-BA had significant effects on flavonoid accumulation in the leave callus of

tenuifolia

while 2

4-D had no significant effect

and MS+ 3.0 mg·L

-1

4-D+ 2.0 mg·L

-1

NAA+ 1.0 mg·L

-1

6-BA was the optimal flavonoid accumulation combination

the three hormones had no significant effect on the accumulation of flavonoids in the root callus of

tenuifolia

and MS+ 2.0 mg·L

-1

4-D+ 1.0 mg·L

-1

NAA+ 1.5 mg·L

-1

6-BA was the best flavonoid accumulation combination.

Conclusion:

Under the conditions

the callus induction rate of roots

stems and leaves of

tenuifolia

is 100%

especially

the callus of

tenuifolia

leaves was the optimal

which is followed by

tenuifolia

stems and

tenuifolia

roots. Under the conditions

the amount of flavonoids in roots

stems and leaves of

tenuifolia

reach 21.31

24.56

23.61 mg·g

-1

respectively.

关键词

Keywords

references

国家药典委员会．中华人民共和国药典．一部 [M]．北京：中国医药科技出版社， 2015 ．

刘丽．中药远志的研究进展概论 [J]．中医临床研究， 2017 ， 9 ( 34 )： 146 - 148 ．

林敬开，闫小平，官仕杰，等．远志不同炮制品皂苷类成分含量的比较 [J]．中国实验方剂学杂志， 2011 ， 17 ( 11 )： 89 - 91 ．

杨改红，程昊，黄群，等． HPLC-MS/MS快速测定远志中远志口山酮Ⅲ和3，6′-二芥子酰基蔗糖的含量 [J]．中国实验方剂学杂志， 2014 ， 20 ( 24 )： 39 - 42 ．

马菁菁，刘斌，罗跃娥．远志化学成分和药理活性的研究进展 [J]．辽宁中医药大学学报， 2009 ， 11 ( 12 )： 161 - 163 ．

王丹丹，白璐，许晓双，等．从农艺性状及化学成分测定分析远志药材商品品规和良种选育的合理性 [J]．中国中药杂志， 2016 ， 41 ( 20 )： 3733 - 3740 ．

易东阳，闫磊，张慧，等．远志的化学成分及治疗阿尔茨海默病的药理作用研究进展 [J]．中国药房， 2014 ， 25 ( 11 )： 1049 - 1051 ．

王晓梅，曹稳根．黄酮类化合物药理作用的研究进展 [J]．宿州学院学报， 2007 ( 1 )： 105-107，41 ．

曹纬国，刘志勤，邵云，等．黄酮类化合物药理作用的研究进展 [J]．西北植物学报， 2003 ( 12 )： 2241 - 2247 ．

刘蕊．黄酮类化合物的药理作用研究进展 [J]．黑龙江医药， 2010 ， 23 ( 2 )： 234 - 236 ．

SHI T X , LI Y G , JIANG Y , et al . Isolation of flavonoids from the aerial parts of Polygala tenuifolia Willd. and their antioxidant activities [J]. J Chin Pharmaceut Sci , 2013 , 22 ( 1 ): 36 .

胡侃．晋产远志种子萌发、组织培养及根显微结构研究 [D]．太原：山西大学， 2008 ．

田伟，周巧梅，谢晓亮，等．远志种子萌发特性的研究 [J]．时珍国医国药， 2008 ， 19 ( 7 )： 1709 - 1710 ．

路平．浅议植物的组织培养 [J]．农技服务， 2017 ， 34 ( 5 )： 30 ．

彭亮，杨冰月，王媛媛，等．远志种苗的质量分级标准 [J]．中国实验方剂学杂志， 2018 ， 24 ( 17 )： 54 - 59 ．

王媛媛，彭亮，肖建玮，等．远志种子质量分级标准考察 [J]．中国实验方剂学杂志， 2018 ， 24 ( 17 )： 33 - 41 ．

叶敏，孔维兵，王小英．追风伞总黄酮提取工艺优选及其体外抗氧化作用考察 [J]．中国实验方剂学杂志， 2013 ， 19 ( 1 )： 44 - 46 ．

张锦阁，闫玉莹，黄莎，等．粗毛淫羊藿幼叶愈伤组织诱导及其总黄酮含量 [J]．贵州农业科学， 2015 ， 43 ( 5 )： 79-82，87 ．

任晓蕾，曹贵阳，初东君，等．核桃楸不同药用部位总黄酮含量测定及变化规律 [J]．中国实验方剂学杂志， 2012 ， 18 ( 24 )： 104 - 106 ．

胡侃．晋产远志种子萌发、组织培养及根显微结构研究 [D]．太原：山西大学， 2008 ．

曲伟红，赵建国，刘可越．细叶远志愈伤组织的诱导 [J]．安徽农业科学， 2011 ， 39 ( 26 )： 15975 - 15976 ．

胡选萍，张晓娟，任鹏斌，等．不同激素对银杏愈伤组织黄酮积累的影响 [J]．中成药， 2015 ， 37 ( 12 )： 2787 - 2790 ．

溥丽华，刘忠华，魏振园，等．国槐种胚愈伤组织培养与异黄酮量的分析 [J]．中草药， 2013 ， 44 ( 14 )： 1984 - 1989 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Hydrogen Peroxide (H₂O₂) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in Polygala tenuifolia Callus

Structure-activity Omics on Anti-inflammatory and Analgesic Effect of Glycyrrhizae Radix et Rhizoma in Qizhiweitong Granules

Structure-activity Omics on Anti-inflammatory and Analgesic Effect of Cyperi Rhizoma in Qizhi Weitong Granules

Analysis of Chemical Constituents in Puerariae Lobatae Radix Dispensing Granules by UPLC-Q-TOF-MS/MS

Effect of Combined Application of Organic Fertilizer and Chemical Fertilizer on Growth， Yield and Quality of Two-year-old Belamcanda chinensis

Related Author

Fu-lin CAO

Ben-xiang HU

Liang PENG

Lu LUO

Jie LI

Ting ZHAO

Yan-ru AN

Tao HUANG

Related Institution

Shaanxi University of Chinese Medicine

China Resources Sanjiu Medical & Pharmaceutical Co. Ltd.

Liaoning Province Modern TCM Research and Engineering Laboratory

Liaoning Multi-dimensional Analysis of TCM Technical Innovation Center

College of Pharmacy， Liaoning University of Traditional Chinese Medicine （TCM）

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰