Study on Immunoregulatory Effect of Compatibility of Salviae Miltiorrhizae Radix et Rhizoma Combined with Sophorae Flavescentis Radix in Immunosuppressive Mice

Yu YANG; Jia-wei WU; Peng CHENG; Zhong-hong WEI; Ai-yun WANG; Wen-xing CHEN; Yin LU

doi:10.13422/j.cnki.syfjx.20191138

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Study on Immunoregulatory Effect of Compatibility of Salviae Miltiorrhizae Radix et Rhizoma Combined with Sophorae Flavescentis Radix in Immunosuppressive Mice

Pharmacology | 更新时间：2021-02-09

- Study on Immunoregulatory Effect of Compatibility of Salviae Miltiorrhizae Radix et Rhizoma Combined with Sophorae Flavescentis Radix in Immunosuppressive Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 13, Pages: 60-65(2019)
- 作者机构：
  
  南京中医药大学　药学院，江苏省中药药效与安全性评价重点实验室，江苏省中医药防治肿瘤协同创新中心，南京　 210023
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191138
  CLC： R285.5; R392; R446.63; R329.2
- Received：08 January 2019，
  
  Published Online：20 February 2019，
  
  Published：05 July 2019
- 稿件说明：
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Yu YANG, Jia-wei WU, Peng CHENG, et al. Study on Immunoregulatory Effect of Compatibility of Salviae Miltiorrhizae Radix et Rhizoma Combined with Sophorae Flavescentis Radix in Immunosuppressive Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 60-65.
DOI：

Yu YANG, Jia-wei WU, Peng CHENG, et al. Study on Immunoregulatory Effect of Compatibility of Salviae Miltiorrhizae Radix et Rhizoma Combined with Sophorae Flavescentis Radix in Immunosuppressive Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 60-65. DOI： 10.13422/j.cnki.syfjx.20191138.

摘要

目的：

通过使用环磷酰胺所致免疫抑制小鼠模型探讨丹参-苦参提取物对免疫缺陷小鼠的免疫调节作用的机制。

方法：

采用环磷酰胺造免疫抑制动物模型，空白组，模型组，丹参-苦参提取物低、中、高剂量组(25，50，100 mg·kg

－1

)，采用小鼠脏器指数评价脏器功能，采用小鼠碳粒廓清模型评价小鼠吞噬细胞功能，采用luminex法检测血清中相关细胞因子水平，流式检测辅助性T细胞数量。体外培养小鼠单核巨噬细胞RAW264.7，给予丹参-苦参提取物后，采用噻唑蓝(MTT)比色法检测丹参-苦参提取物对RAW264.7细胞增殖的影响，采用实时荧光定量聚合酶链式反应(Real-time PCR)对其增殖的影响及其可能机制进行探讨。

结果：

与空白组比较，模型组显著抑制肝指数(

0.01)和脾脏指数(

0.05)，动物血清白细胞介素-6(IL-6)的分泌明显降低(

0.05)，吞噬指数显著下降(

0.01)，同时50 mg·L

－1

环磷酰胺显著抑制RAW264.7细胞的增殖(

0.01)；与模型组比较，丹参-苦参提取物中、高剂量均可以显著提高肝脏指数(

0.01)，并显著促进趋化因子C-X-C配体1(CXCL1)的分泌(

0.01)，丹参-苦参提取物高剂量可以显著促进IL-6的分泌(

0.01)，丹参-苦参提取物中、高剂量能够明显提高小鼠腋下以及颈部淋巴结中辅助性T细胞的数量(

0.05，

0.01)，显著性升高吞噬指数的趋势(

0.01)，显著性促进RAW264.7细胞的增殖(

0.01)，且促进IL-6信号传导与转录激活因子(STAT3)调控下游B淋巴细胞瘤-2(Bcl-2)，Bcl-2样蛋白1(Bcl-2L1)以及细胞周期蛋白D

(CCND1)mRNA的表达，起抗凋亡作用。

结论：

丹参-苦参提取物可对抗环磷酰胺所导致的免疫抑制，增强动物免疫功能。

Abstract

Objective:

To investigate the mechanism of immunomodulatory effect of extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix on immunodeficiency mice by immunosuppressive mouse model induced by cyclophosphamide.

Method:

An immunosuppressive animal model was established by cyclophosphamide. The blank group

the model group

the low

medium and high dose group of Salvia miltiorrhiza and Sophora extract groups (25

100 mg·kg

-1

)

using mouse organs organ evaluation index using a mouse model to evaluate the carbon clearance phagocytic cell function using luminex to detect levels of relevant cytokines in serum and using flow to detect the number of helper T cells. Mouse mononuclear macrophage leukemia cells (RAW264.7) were cultured

in vitro

and the effect of Salvia miltiorrhiza extracts on the proliferation of RAW264.7 cells were detected by methylthiazolyldiphenyl-tetrazolium bromide(MTT)assay. The proliferation was induced by Real-time PCR. The impact and its possible mechanisms are explored.

Result:

Compared with blank group

cyclophosphamide significantly inhibited liver index (

0.01) and spleen index (

0.05)

and the secretion of serum interleukin-6 (IL-6) was significantly decreased (

0.05)

the phagocytic index decreased significantly (

0.01)

while 50 mg·L

-1

cyclophosphamide significantly inhibited the proliferation of mononuclear macrophage RAW264.7 (

0.01). Compared with model group

high dose of extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix can significantly increase the liver index (

0.01). The high dose of extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix can significantly promote the chemokine CXC motif ligand 1 (CXCL1

0.01)

high dose can significantly promote the secretion of IL-6 (

0.01). The high dose of extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix can significantly improve the auxiliary in the axillary and cervical lymph nodes of mice. The number of T cells (

0.05

0.01)

the high dose of extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix could significantly increase the phagocytic index (

0.01). Medium and high saliency promoted the proliferation of RAW264.7 cells (

0.01)

and promoted IL-6 signaling and activator of transcription 3 (STAT3) regulation of the downstream B lymphoma-2 gene (Bcl-2)

Bcl-2 like protein 1 (Bcl-2L1) and the cyclin D

gene (CCND1) plays an anti-apoptotic role.

Conclusion:

The extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix can counteract the immunosuppression caused by cyclophosphamide and enhance the immune function of animals.

关键词

Keywords

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