Analysis on Fingerprint of Achyranthis Bidentatae Radix

Xian-kui LIANG; Jing-wei LEI; Hai-yan GONG; Wei-wei TANG; Cai-xia XIE; Rui-juan LI

doi:10.13422/j.cnki.syfjx.201911914

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Analysis on Fingerprint of Achyranthis Bidentatae Radix

Resource and Quality Evaluation | 更新时间：2021-02-09

- Analysis on Fingerprint of Achyranthis Bidentatae Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 19, Pages: 109-116(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.201911914
  CLC： R284.2; R285; R22; R2-031
- Received：27 December 2018，
  
  Published Online：17 June 2019，
  
  Published：05 October 2019
- 稿件说明：
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Xian-kui LIANG, Jing-wei LEI, Hai-yan GONG, et al. Analysis on Fingerprint of Achyranthis Bidentatae Radix[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 109-116.
DOI：

Xian-kui LIANG, Jing-wei LEI, Hai-yan GONG, et al. Analysis on Fingerprint of Achyranthis Bidentatae Radix[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 109-116. DOI： 10.13422/j.cnki.syfjx.201911914.

摘要

目的：

对不同产地牛膝药材、同一产地不同规格牛膝药材的HPLC指纹图谱进行研究，比较不同产地牛膝药材、同一产地不同规格牛膝药材的HPLC指纹图谱差异，探讨产地、规格对牛膝药材质量的影响及规格与牛膝药材内部质量的联系；为牛膝药材的产地鉴别提供理论依据；为牛膝药材质量标准制定、规格完善及临床用药提供参考。

方法：

采用超声提取法制备样品，通过HPLC法采集不同产地牛膝药材、同一产地不同规格牛膝药材的指纹图谱，运用相似度、聚类分析、主成分分析的方法进行分析，并比较不同产地牛膝药材、不同规格牛膝药材的HPLC指纹图谱差异。

结果：

不同产地分析，主成分分析能将5个产地牛膝药材区分开，且产地鉴别结果优于聚类分析和相似度分析。不同规格分析，相似度和主成分分析均不能将不同规格牛膝药材区分开。

结论：

不同产地牛膝药材化学成分种类、峰高差异显著；不同规格牛膝药材化学成分种类、峰高差异较小；主成分分析可用于牛膝药材的产地鉴别。

Abstract

Objective:

To study HPLC fingerprints of Achyranthis Bidentatae Radix from different origins

compare different specifications in the same origin

and explore the effect of origin and specifications on the quality of Achyranthis Bidentatae Radix and relationship between the specifications and the internal quality of Achyranthis Bidentatae Radix

in order to provide basis for the identification of its origin.

Method:

The HPLC fingerprints of Achyranthis Bidentatae Radix from different origins and with different specifications in the same origin were collected. The similarity analysis

cluster analysis and principal component analysis were adopted to analyze the fingerprints

the differences in fingerprints of Achyranthis Bidentatae Radix from different origins and with different specifications in the same origin were compared.

Result:

Analysis of different origins and principal component analysis could be used to distinguish Achyranthis Bidentatae Radix from five producing areas

and the identification results of origin analysis was better than those of cluster analysis and similarity analysis. Analysis of different specifications

similarity analysis or principal component analysis could not distinguish Achyranthis Bidentatae Radix with different specifications.

Conclusion:

There are significant differences in chemical composition and peak height among Achyranthis Bidentatae Radix from different origins

with less differences in chemical composition and peak height of Achyranthis Bidentatae Radix with different specifications

the principal component analysis could be used to identify origins of Achyranthis Bidentatae Radix.

关键词

Keywords

references

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