Effect of Modified Sijunzi Tang on Protein and mRNA Expressions of Occludin, ZO-1 and Claudin-1 in Cerebral Ischemia Rats

Bing-rong TANG; Hua LI; Wang-hua LIU; Kang-li HU

doi:10.13422/j.cnki.syfjx.20191201

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Effect of Modified Sijunzi Tang on Protein and mRNA Expressions of Occludin, ZO-1 and Claudin-1 in Cerebral Ischemia Rats

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- Effect of Modified Sijunzi Tang on Protein and mRNA Expressions of Occludin, ZO-1 and Claudin-1 in Cerebral Ischemia Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 15, Pages: 57-63(2019)
- 作者机构：
  
  湖南中医药大学　中医诊断学湖南省重点实验室，长沙　 410208
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191201
  CLC： R2-0; R22; R285.5; R289
- Received：19 November 2018，
  
  Published Online：01 March 2019，
  
  Published：05 August 2019
- 稿件说明：
移动端阅览
Bing-rong TANG, Hua LI, Wang-hua LIU, et al. Effect of Modified Sijunzi Tang on Protein and mRNA Expressions of Occludin, ZO-1 and Claudin-1 in Cerebral Ischemia Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 57-63.
DOI：

Bing-rong TANG, Hua LI, Wang-hua LIU, et al. Effect of Modified Sijunzi Tang on Protein and mRNA Expressions of Occludin, ZO-1 and Claudin-1 in Cerebral Ischemia Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 57-63. DOI： 10.13422/j.cnki.syfjx.20191201.

摘要

目的：

探讨加味四君子汤对脑缺血大鼠脑组织闭合蛋白(Occludin)，密封蛋白-1(Claudin-1)，闭锁连接蛋白-1(ZO-1)表达的影响，并探讨相关的作用机制。

方法：

将60只雄性SD大鼠随机分为假手术组，模型组，依达拉奉组(3.2×10

－3

g·kg

－1

)，加味四君子汤加减低、中、高剂量组(4.77，9.54，19.08 g·kg

－1

)。采用线栓法制备大脑中动脉栓塞(MCAO)模型，治疗7 d后处死，运用蛋白免疫印迹法(Western blot)检测大鼠缺血侧脑皮质区Occludin，ZO-1，Claudin-1蛋白的表达，运用实时荧光定量聚合酶链式反应(Real-time PCR)检测大鼠缺血侧脑皮质区Occludin，ZO-1，Claudin-1 mRNA表达。

结果：

与假手术组比较，模型组Occludin，Claudin-1，ZO-1蛋白显著减少(

0.01)；与模型组比较，依达拉奉组、加味四君子汤各剂量组Occludin，Claudin-1，ZO-1蛋白均显著增高(

0.01)。与假手术组比较，模型组大鼠Occludin，Claudin-1，ZO-1 mRNA表达下调(

0.01)；与模型组比较，依达拉奉组、加味四君子汤各剂量组大鼠Occludin，Claudin-1，ZO-1 mRNA表达上升(

0.01)。

结论：

加味四君子汤可能通过增加紧密连接蛋白Occludin，ZO-1，Claudin-1的表达，保护血脑屏障，减轻缺血性脑卒中大鼠脑水肿。

Abstract

Objective:

To investigate the effect of modified Sijunzi Tang on protein and mRNA expressions of Occludin

Claudin-1 and zonula occludens-1(ZO-1) in cerebral ischemia rats.

Method:

Totally 60 male Sprague-Dawley rats were randomly divided into sham operation group

model group

edaravone group

small-dose modified Sijunzi Tang group

middle-dose modified Sijunzi Tang group and high-dose modified Sijunzi Tang group. The middle cerebral artery occlusion (MCAO) model was prepared by suture method. After 7 days of treatment

the modeling group was put to death. Western blot was used to detect the contents of Occludin

Claudin-1 and ZO-1 in the ischemic cerebral cortex of rats. Detection of Occludin

ZO-1

Claudin-1 mRNA expression in the ischemic cortex of rats by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR).

Result:

Compared with the sham operation group

protein expressions of Occludin

Claudin-1

and ZO-1 in the model group were significantly down-regulated (

0.01). Compared with the model group

protein expressions of Occludin

ZO-1

Claudin-1 in the positive control group and modified Sijunzi Tang groups increased significantly

with statistically significant differences (

0.01). Compared with the Sham operation group

the expression of Occludin

Claudin-1

and ZO-1 mRNA in the model group was down-regulated (

0.01). Compared with the model group

mRNA expressions of Occludin

Claudin-1

ZO-1 in the positive control group and modified Sijunzi Tang groups increased

with statistically significant differences (

0.01).

Conclusion:

Modified Sijunzi Tang may protect the blood-brain barrier and reduce brain edema in ischemic stroke rats by increasing the expression of tight junction proteins Occludin

ZO-1 and Claudin-1.

关键词

Keywords

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