Effect of Chuankezhi Injection on Prevention and Treatment of H1N1 Influenza Virus in Mice

Rong-mei YAO; Xin MAO; Tian-ge QU; Yan-yan BAO; Jing SUN; Han SHI; Yu-jing SHI; Xiao-lan CUI

doi:10.13422/j.cnki.syfjx.20191236

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Effect of Chuankezhi Injection on Prevention and Treatment of H1N1 Influenza Virus in Mice

Pharmacology | 更新时间：2021-02-09

- Effect of Chuankezhi Injection on Prevention and Treatment of H1N1 Influenza Virus in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 12, Pages: 43-49(2019)
- 作者机构：
  
  1.中国中医科学院　中药研究所，北京　 100700；
  2.华北理工大学　中医学院，河北　唐山　 063210；
  3.北京中医药大学　基础医学院，北京　 100029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191236
  CLC： R289; R373.1; R322.3; R285.5
- Received：18 September 2018，
  
  Published Online：05 March 2019，
  
  Published：20 June 2019
- 稿件说明：
移动端阅览
Rong-mei YAO, Xin MAO, Tian-ge QU, et al. Effect of Chuankezhi Injection on Prevention and Treatment of H1N1 Influenza Virus in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 43-49.
DOI：

Rong-mei YAO, Xin MAO, Tian-ge QU, et al. Effect of Chuankezhi Injection on Prevention and Treatment of H1N1 Influenza Virus in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 43-49. DOI： 10.13422/j.cnki.syfjx.20191236.

摘要

目的：

评价喘可治注射液对甲型H1N1流感病毒FM1株致小鼠肺炎模型的药效作用。

方法：

将ICR小鼠随机分为正常组、模型组、达菲组(27.5 mg·kg

－1

·d

－1

)、喘可治注射液组(1.5 mL·kg

－1

·d

－1

)。除正常组外，其余各组采用甲型H1N1流感病毒FM1株滴鼻感染ICR小鼠造成病毒性肺炎模型，分别进行死亡保护实验、治疗性给药实验和预防性给药实验。死亡保护实验中每组20只小鼠，以2倍半数致死量(LD

)流感病毒液滴鼻感染，感染后当天开始给药，每天1次，连续4 d，观察感染后14 d内动物的死亡情况，并计算死亡率、死亡保护率；治疗性给药实验中每组10只小鼠，以0.8倍LD

流感病毒液滴鼻感染，感染后当天开始给药，每天1次，连续4 d，于实验第5天处死小鼠，采用酶联免疫吸附测定(ELISA)检测肺组织中白细胞介素-8(IL-8)含量及脑组织内发热介质前列腺素E

(PGE

)，精氨酸加压素(AVP)的含量，实时荧光定量聚合酶链式反应(Real-time PCR)检测肺组织内相对病毒载量；预防性给药实验中每组10只小鼠，分组当天开始给药，每天1次，连续5 d，末次给药1 h后，除正常组外，其余各组以0.8倍LD

流感病毒滴鼻感染ICR小鼠，感染后第4天称重后处死小鼠，计算各组肺指数、脾指数、胸腺指数及肺指数抑制率，Real-time PCR检测肺组织内相对病毒载量。

结果：

与正常组比较，模型组小鼠IL-8，PGE2含量，肺指数及肺组织内流感病毒株病毒载量均显著升高(

0.01)；与模型组比较，治疗性给予喘可治注射液可明显降低感染后小鼠的死亡率、肺组织内IL-8含量、脑组织内PGE

含量及肺组织中流感病毒病毒载量(

0.05，

0.01)；预防性给予喘可治注射液可显著提高感染小鼠的胸腺指数(

0.01)，明显降低感染小鼠肺指数及肺组织中流感病毒病毒载量(

0.05)；两种给药方式均可明显减轻支气管、血管周围及肺泡间质等组织炎性细胞浸润，减少管腔内红细胞渗出。

结论：

喘可治注射液对甲型H1N1流感病毒感染所致病毒性肺炎小鼠模型有较好的治疗和预防作用，其作用机制可能与减轻炎症反应和抑制病毒复制有关。

Abstract

Objective:

To evaluate the effect of Chuankezhi injection on mouse model of pneumonia induced by influenza A (H1N1) FM1 strain.

Method:

ICR mice were randomly divided into normal group

model group

tamiflu control group (27.5 mg·kg

-1

·d

-1

) and Chuankezhi injection group (1.5 mL·kg

-1

·d

-1

). In the death protection experiment

mice were infected with 2×half lethal dose (LD

) of influenza virus FM1.The Chuankezhi injection was given once a day for 4 days. The number of death animal within 14 days was counted. The mortality and the death protection rate were calculated. In the treatment experiment

mice were infected with 0.8×LD

of influenza virus

and the Chuankezhi injection was given once a day for 4 days. On the 5

day after the infection

the levels of interleukin-8 (IL-8) in lung

prostaglandin E

(PGE

) and vasopressin (AVP) in brain were tested by enzyme-linked immunosorbent assay (ELISA). The viral load of influenza virus in lung was tested by Real-time PCR. In the pre-treatment experiment

mice were given Chuankezhi injection once a day for 5 days. 1 hour after the last treatment

mice were infected with 0.8×LD

influenza virus. 4 days after the infection

the lung index

spleen index

thymus index

and viral load in lung tissue were calculated.

Result:

Compared with normal group

the IL-8

PGE

content

lung index and viral load in the lung tissue of model group were significantly increased (

0.01). Compared with model group

Treatment of Chuankezhi injection could reduce the rate of deaths. Significantly inhibit the level of IL-8

PGE

and the viral load of influenza(

0.05

0.01). Pre-treatment of Chuankezhi injection could significantly increase the thymus index of infected mice(

0.01)

reduce lung index and the viral load of influenza(

0.05). Both administration methods can significantly reduce the infiltration of inflammatory cells in the bronchial

perivascular and alveolar interstitium and reduce the exudation of red blood cells in the lumen.

Conclusion:

Chuankezhi injection could effectively prevent the mouse model of pneumonia induced by influenza A (H1N1) virus. The mechanism might be related to the reduction of inflammation and inhibiting viral replication.

关键词

Keywords

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