Correlation Between Effective Components Content and Color Values of Gentianae Radix et Rhizoma Based on Color Difference Principle

Ying SU; Di LI; Xiao-lin HOU; Li-li WENG

doi:10.13422/j.cnki.syfjx.20191314

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Correlation Between Effective Components Content and Color Values of Gentianae Radix et Rhizoma Based on Color Difference Principle

Resource and Quality Evaluation | 更新时间：2021-02-09

- Correlation Between Effective Components Content and Color Values of Gentianae Radix et Rhizoma Based on Color Difference Principle
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 13, Pages: 151-156(2019)
- 作者机构：
  
  长春中医药大学，长春　 130117
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191314
  CLC： R284.1; R289; R22; R2-031
- Received：01 November 2018，
  
  Published Online：20 March 2019，
  
  Published：05 July 2019
- 稿件说明：
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Ying SU, Di LI, Xiao-lin HOU, et al. Correlation Between Effective Components Content and Color Values of Gentianae Radix et Rhizoma Based on Color Difference Principle[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 151-156.
DOI：

Ying SU, Di LI, Xiao-lin HOU, et al. Correlation Between Effective Components Content and Color Values of Gentianae Radix et Rhizoma Based on Color Difference Principle[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 151-156. DOI： 10.13422/j.cnki.syfjx.20191314.

摘要

目的：

通过测定龙胆色差值及有效成分龙胆苦苷的含量，将代表颜色的外观指标值与代表质量的内在含量指标值相关联，探讨有效成分含量与颜色之间的相关性，为常用中药龙胆的质量评价提供参考依据。

方法：

利用色差仪对龙胆粉末进行测定，以2015年版《中国药典》中龙胆含量测定项下方法，采用HPLC进行测定，通过SPSS 21.0软件进行统计分析。

结果：

龙胆中龙胆苦苷含量与

(代表颜色深浅)，

(色差值)存在极显著正相关关系(

0.01)，表明在一定程度上，

，

值越大，龙胆苦苷含量越高；而龙胆苦苷含量与

(代表颜色红绿色度)，

(代表颜色黄蓝色度)存在极显著负相关关系(

0.01)，表明在一定程度上，

，

值越小，龙胆苦苷含量越高。

结论：

龙胆外观颜色与有效成分龙胆苦苷含量有关，且颜色偏浅黄色的龙胆有效成分含量较高，质量相对较好。通过色差仪对龙胆色差值进行测定，快速预测龙胆中有效成分的含量，可为该药材质量评价体系的建立提供参考依据。

Abstract

Objective:

To measure the color values of Gentianae Radix et Rhizoma and the contents of gentiopicroside

the appearance index value of the representative color was correlated with the intrinsic index value representing the quality

in order to explore the correlation between the contents of active ingredients and the color values

and provide basis for the quality evaluation of Gentianae Radix et Rhizoma.

Method:

The color difference values of Gentianae Radix et Rhizoma powder was measured by colorimeter.The content determination method of gentiopicroside in the 2015 edition of

China Pharmacopoeia

was adopted. The content of gentiopicroside was determined by HPLC

and the correlation and regression analysis was carried out by SPSS 21.0 software.

Result:

There was a significantly positive correlation between the contents of gentiopicroside and

(representing colorshade)and

(representing total color difference)(

0.01)

while the contents of gentiopicroside and

(representing color red-green direction) and

(representing color yellow-blue direction) had a significantly negative correlation (

0.01).

Conclusion:

The color value of Gentianae Radix et Rhizoma has a certain correlation with the content of gentiopicroside

and yellowish Gentianae Radix et Rhizoma contain more active ingredients

with a better quality.The contents of gentiopicroside active ingredients in Gentianae Radix et Rhizoma can be quickly predicted by determining the color difference values

which can provide a new idea for quality evaluation of this herb.

关键词

Keywords

references

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