Effect of Qingzao Jiufei Tang on Rate-limiting Enzymes HK2, PFK2 and PKM2 in Glycolytic Pathway in Lewis Lung Cancer-bearing Mice Cells

Gong YU; Jiang-tao CHEN; Qiao HU; Bin RAO; Bin XIE

doi:10.13422/j.cnki.syfjx.20191325

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Effect of Qingzao Jiufei Tang on Rate-limiting Enzymes HK2, PFK2 and PKM2 in Glycolytic Pathway in Lewis Lung Cancer-bearing Mice Cells

Topic on Anti-lung Cancer of Qingzao Jiufei Tang | 更新时间：2021-02-09

- Effect of Qingzao Jiufei Tang on Rate-limiting Enzymes HK2, PFK2 and PKM2 in Glycolytic Pathway in Lewis Lung Cancer-bearing Mice Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 4, Pages: 54-58(2020)
- 作者机构：
  
  江西中医药大学，南昌　 330004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191325
  CLC： R22; R242; R2-031; R285.5
- Received：07 March 2019，
  
  Published Online：20 March 2019，
  
  Published：20 February 2020
- 稿件说明：
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Gong YU, Jiang-tao CHEN, Qiao HU, et al. Effect of Qingzao Jiufei Tang on Rate-limiting Enzymes HK2, PFK2 and PKM2 in Glycolytic Pathway in Lewis Lung Cancer-bearing Mice Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 54-58.
DOI：

Gong YU, Jiang-tao CHEN, Qiao HU, et al. Effect of Qingzao Jiufei Tang on Rate-limiting Enzymes HK2, PFK2 and PKM2 in Glycolytic Pathway in Lewis Lung Cancer-bearing Mice Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 54-58. DOI： 10.13422/j.cnki.syfjx.20191325.

摘要

目的：

观察清燥救肺汤对荷Lewis小鼠肺癌细胞糖酵解关键限速酶己糖激酶2(hexokinase 2，HK2)，6-磷酸果糖激酶2(phosphofructokinase，PFK2)，M2型丙酮酸激酶(pyruvate kinase isoform M2，PKM2)的表达及葡萄糖含量的影响。

方法：

雄性C57BL/6J小鼠50只，随机分为模型组，环磷酰胺(cyclophosphamide，CTX)组，清燥救肺汤高、中、低剂量组，每组10只。所有小鼠右腋下注射Lewis肺癌细胞建立肺癌荷瘤模型，清燥救肺汤高、中、低剂量组剂量分别为11，5.5，2.75 g·kg

-1

·d

-1

，造模前2周开始灌胃给药，CTX组以50 mg·kg

-1

·(2 d)

-1

腹腔注射给药，模型组以等体积生理盐水灌胃给药，接种后继续给药2周，处死小鼠取瘤组织；氧化酶法检测葡萄糖含量；实时荧光定量聚合酶链式反应(Real-time PCR)检测HK2 mRNA表达；蛋白质免疫印迹法(Western blot)检测PFK2蛋白表达；酶联免疫吸附测定(enzyme-linked immunesorbent assay，ELISA)检测PKM2活性。

结果：

与模型组比较，清燥救肺汤高、中、低剂量组肺癌细胞HK2 mRNA表达及PKM2活性显著降低，葡萄糖含量显著升高(

0.01)；与模型组比较，清燥救肺汤高、中剂量组肺癌细胞PFK2蛋白表达明显降低(

0.05，

0.01)。

结论：

清燥救肺汤可有效抑制荷Lewis肺癌细胞增殖，降低肺癌细胞葡萄糖摄取速率，糖酵解关键限速酶HK2，PFK2，PKM2可能是其药效作用靶点。

Abstract

Objective:

To study the effect of Qingzao Jiufei Tang on the expression of key limiting enzymes hexokinase 2(HK2)

phosphofructokinase 2(PFK2) and pyruvate kinase M2 (PKM2)

and the glucose content in Lewis mice colon cancer cells.

Method:

A total of 50 male C57BL/6J mice were randomly divided into model group

chemotherapy group

and high

middle and low-dose Qingzao Jiufei Tang groups

with 10 mice in each group. The lung cancer cell model was established by injecting Lewis lung cancer cells into the right axilla. The high

middle and low dose groups were administered at the doses of 11

5.5

2.75 g·kg

-1

·d

-1

for 2 weeks before modeling. The drug was administered through intraperitoneal injection at a dose of 50 mg·kg

-1

·(2 d)

-1

in the chemotherapy group. The model group was intragastrically administered with an equal volume of normal saline. After the inoculation

the drug was administered for two weeks. Two weeks later

all of the mice were put to death

and tumor tissues were collected. The mRNA expression of HK2 was detected by Real-time PCR. the protein expression of PFK2 was detected by Western blot

the PKM2 activity was detected by enzyme-linked immunosorbent assay (ELISA).

Result:

Compared with the model group

mRNA expressions and activity of PKM2 in lung cancer cells of treatment groups were significantly declined

and glucose content increased significantly

with significant differences from those of model group (

0.01). The PFK2 protein expressions in lung cancer cells of treatment groups (high

medium and low-dose groups) were significantly decreased (

0.05

0.01).

Conclusion:

Qingzao Jiufei Tang could inhibit Lewis proliferation

and decrease the glucose intake in lung cancer cells. The effect targets may be the key rate-limiting enzymes HK2

PFK2

PKM2.

关键词

Keywords

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Related Author

Jia-ping LI

Bin XIE

Gong YU

Qiao HU

Jia-ping LI

XIE Xiong

XIE Bin

RAO Bin

Related Institution

Henan Province Hospital of Traditional Chinese Medicine

The Second Clinical Medical College， Henan University of Chinese Medicine

The Affiliated Cancer Hospital of Zhengzhou University/Henan Cancer Hospital

Henan Province Hospital of Traditional Chinese Medicine/The Second Affiliated Hospital of Henan University of Chinese Medicine

School of Basic Medicine， Gansu University of Chinese Medicine

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