Effect of Pudilan Xiaoyan Oral Liquid on Acute Lung Injury Rat Induced by Lipopolysaccharide

Yi-fei YANG; Guang-ping ZHANG; Yun-hang GAO; Meng MA; Shuang-rong GAO; Han LI; Ling SONG; Zu-guang YE

doi:10.13422/j.cnki.syfjx.20191338

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Effect of Pudilan Xiaoyan Oral Liquid on Acute Lung Injury Rat Induced by Lipopolysaccharide

Pharmacology | 更新时间：2021-02-09

- Effect of Pudilan Xiaoyan Oral Liquid on Acute Lung Injury Rat Induced by Lipopolysaccharide
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 13, Pages: 55-59(2019)
- 作者机构：
  
  中国中医科学院　中药研究所，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191338
  CLC： R285; R563.1; R364.5; R361+ .2
- Received：18 December 2018，
  
  Published Online：19 March 2019，
  
  Published：05 July 2019
- 稿件说明：
移动端阅览
Yi-fei YANG, Guang-ping ZHANG, Yun-hang GAO, et al. Effect of Pudilan Xiaoyan Oral Liquid on Acute Lung Injury Rat Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 55-59.
DOI：

Yi-fei YANG, Guang-ping ZHANG, Yun-hang GAO, et al. Effect of Pudilan Xiaoyan Oral Liquid on Acute Lung Injury Rat Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 55-59. DOI： 10.13422/j.cnki.syfjx.20191338.

摘要

目的：

探讨蒲地蓝消炎口服液(PDL)对脂多糖(LPS)导致的大鼠急性肺损伤的影响及作用机制。

方法：

72只Wistar大鼠，按体质量随机分为空白组，模型组，地塞米松组，PDL 7，3.5，1.75 g·kg

－1

·d

－1

组。模型组，地塞米松组，PDL 7，3.5，1.75 g·kg

－1

·d

－1

组雾化吸入LPS制备大鼠急性肺损伤模型。检测各组大鼠肺泡灌洗液(BALF)中白细胞总数，酶联免疫吸附测定(ELISA)检测肺组织中核转录因子-

B(NF-

B)，白细胞介素-10(IL-10)的含量，苏木素-伊红(HE)染色观察肺组织形态学改变，探讨不同剂量PDL对急性肺损伤大鼠的影响。

结果：

与模型组比较，PDL 7，3.5 g·kg

－1

·d

－1

组BALF中白细胞总数明显降低(

0.05)；PDL 7，3.5，1.75 g·kg

－1

·d

－1

组NF-

B表达量明显降低(

0.05)；PDL 3.5 g·kg

－1

·d

－1

组IL-10表达量明显升高(

0.05)；PDL 7 g·kg

－1

·d

－1

组肺组织中炎症反应减轻，水肿、瘀血等病理改变明显缓解(

0.05)。

结论：

PDL对急性肺损伤模型具有保护作用，可减轻大鼠急性肺损伤模型中肺组织的炎症反应，其机制与NF-

B，IL-10的表达相关，对肺组织的病理损伤有修复作用。

Abstract

Objective:

To explore the effect and mechanism of Pudilan Xiaoyan oral liquid(PDL) on the acute lung injury rat induced by lipopolysaccharide (LPS).

Method:

The 72 Wistar rats were randomly divided into control group

model group

dexamethasone group

PDL 7

3.5

1.75 g·kg

-1

·d

-1

group according to body weight.The acute lung injury model was made through inhalation with lipopolysaccharide in the model group

hexadecadrol group

PDL 7

3.5

1.75 g·kg

-1

·d

-1

group.To examining each rat alveolar lavage fluid (BALF) of the total number of white blood cells

enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of nuclear transcription factors-kappa B (NF-

B) and interleukin-10 (IL-10). Hematoxylin-eosin(HE) staining was used to observe morphological changes of lung tissue and explore different doses of PDL effect on acute lung injury in rats.

Result:

Compared with model group

the account of leukocyte in BALF decreased significantly in PDL 7 g·kg

-1

·d

-1

group and PDL 3.5 g·kg

-1

·d

-1

group (

0.05). The expression of NF-

B significantly decreased in PDL 7

3.5

1.75 g·kg

-1

·d

-1

group (

0.05). And the expression of IL-10 significantly increased in PDL 3.5 g·kg

-1

·d

-1

group (

0.05). In PDL 7 g·kg

-1

·d

-1

group

the inflammation

edema and congestion in lung tissue reduced (

0.05).

Conclusion:

PDL has a significant protective effect on the inflammation of acute lung injury model

and its mechanism is related to the expressions of NF-

B and IL-10. PDL could also repair the injury of lung in acute lung injury model.

关键词

Keywords

references

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