Effect of Different Processed Products of Crataegi Fructus on Dyspepsia by <sup>1</sup>H-NMR Metabonomics

Chun-xia NIE; Yan-yan HAO; Pan HE; Cong LIU; Yan NI; Xu-liang HAO

doi:10.13422/j.cnki.syfjx.20191353

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Effect of Different Processed Products of Crataegi Fructus on Dyspepsia by ¹H-NMR Metabonomics

Drug Metabolism | 更新时间：2021-02-09

- Effect of Different Processed Products of Crataegi Fructus on Dyspepsia by ¹H-NMR Metabonomics
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 13, Pages: 111-118(2019)
- 作者机构：
  
  1.山西中医药大学，太原　 030619；
  2.山西省中医药研究院，太原　 030045
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191353
  CLC： R24; R934.1; R969.1; R28; C37
- Received：28 January 2019，
  
  Published Online：19 March 2019，
  
  Published：05 July 2019
- 稿件说明：
移动端阅览
Chun-xia NIE, Yan-yan HAO, Pan HE, et al. Effect of Different Processed Products of Crataegi Fructus on Dyspepsia by ¹H-NMR Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 111-118.
DOI：

Chun-xia NIE, Yan-yan HAO, Pan HE, et al. Effect of Different Processed Products of Crataegi Fructus on Dyspepsia by ¹H-NMR Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 111-118. DOI： 10.13422/j.cnki.syfjx.20191353.

摘要

目的：

采用

H-NMR技术的血清代谢组学方法，并结合传统生化指标分析，研究炮制对山楂促消化作用的影响。

方法：

实验动物喂饲特制高蛋白、高热量饲料复制食积模型，以小鼠小肠推进率和大鼠胃肠道激素水平作为药效学指标，应用

H-NMR测定大鼠血清，结合多元统计分析方法对比给予大鼠净山楂、炒山楂、焦山楂后血清代谢物的变化，比较各炮制品对食积模型大鼠的治疗作用。

结果：

山楂各炮制品组对于食积因素引起的小鼠肠推进障碍及胃肠激素分泌紊乱均有改善作用，且以焦山楂组改善效果最为显著。大鼠血清代谢组学结果显示，模型组与空白组存在明显的代谢差异，根据S-plot图的变量重要性投影(VIP)值、单因素ANOVA以及受试者工作特征曲线(ROC曲线)下的面积(AUC)筛选出3-羟基丁酸，甘油磷酰胆碱(GPC)，

-乙酰糖蛋白，

-乙酰糖蛋白，氧化三甲胺，丙氨酸，乙酸，谷氨酸，谷氨酰胺，肌酸，亮氨酸，乳酸，葡萄糖共13个与食积相关的生物标志物。比较给药后山楂各炮制品组引起的代谢变化，发现焦山楂组对

-乙酰糖蛋白、谷氨酰胺和GPC的回调趋势更接近空白组，其余代谢产物组成与净山楂组和炒山楂组基本一致。

结论：

山楂炮制后消食导滞作用存在一定差异，焦山楂改善食积疗效最为显著，可能主要通过调节胃肠动力障碍及能量代谢等发挥作用。

Abstract

Objective:

The effect of processing on promoting digestion of Crataegi Fructus was investigated by the method of serum metabonomics based on

H-NMR and the analysis of traditional biochemical indexes.

Method:

The dyspepsia model was induced by high protein and calorie diet. The small intestine propulsive rate in mice and the hormone level in rat gastrointestinal tract were used as pharmacodynamic indexes. After administration of Crataegi Fructus

fried and charred Crataegi Fructus

the changes of serum metabolites were analyzed by

H-NMR technique combined with multivariate statistical analysis

so as to compare the therapeutic effects of each processed products on rats with dyspepsia model.

Result:

Each processed products group of Crataegi Fructus could improve the ability of intestinal propulsion and gastric emptying

especially in charred Crataegi Fructus group

caused by dyspepsia. The serum metabonomics proved that there were significant metabolic differences between the model group and the blank group. A total of 13 biomarkers related to dyspepsia

covering 3-hydroxybutyric acid

glycerophosphoryl choline (GPC)

-acetylglycoprotein

trimethylamine oxide (TMAO)

alanine

acetic acid

glutamic acid

glutamine

creatine

leucine

lactic acid and glucose

were screened on the grounds of VIP value of S-plot

single factor ANOVA and area under receiver operating characteristic curve (ROC curve) (AUC). The metabolite composition of each administration group was the same except that the callback tendency of

-acetylglycoprotein

glutamine and GPC in charred Crataegi Fructus group was closer to that in the blank group.

Conclusion:

There are differences in the effect of promoting digestion to eliminate stagnation among processed products of Crataegi Fructus

charred Crataegi Fructus is more effective in improving dyspepsia

which may play a role in regulating gastrointestinal motility and energy metabolism.

关键词

Keywords

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Effect of Different Processed Products of Crataegi Fructus on Dyspepsia by 1H-NMR Metabonomics

DOI：10.13422/j.cnki.syfjx.20191353

摘要

Abstract

关键词

Keywords

references

Effect of Different Processed Products of Crataegi Fructus on Dyspepsia by ¹H-NMR Metabonomics