Protective Effect and Mechanisms of Geraniin on D-galactosamine-induced Liver Injury in Mice

Jia-ni LI; Zi-meng LI; Ya GAO; Ming-li ZHONG; Qiu-yan CAO; Dan GUO; Ke-feng ZHANG

doi:10.13422/j.cnki.syfjx.20191403

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Protective Effect and Mechanisms of Geraniin on D-galactosamine-induced Liver Injury in Mice

Pharmacology | 更新时间：2021-02-09

- Protective Effect and Mechanisms of Geraniin on D-galactosamine-induced Liver Injury in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 15, Pages: 116-121(2019)
- 作者机构：
  
  1.桂林医学院，广西　桂林　 541004；
  2.甘肃中医药大学，兰州　 730000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191403
  CLC： R2-0; R22; R285.5
- Received：24 December 2018，
  
  Published Online：08 April 2019，
  
  Published：05 August 2019
- 稿件说明：
移动端阅览
Jia-ni LI, Zi-meng LI, Ya GAO, et al. Protective Effect and Mechanisms of Geraniin on D-galactosamine-induced Liver Injury in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 116-121.
DOI：

Jia-ni LI, Zi-meng LI, Ya GAO, et al. Protective Effect and Mechanisms of Geraniin on D-galactosamine-induced Liver Injury in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 116-121. DOI： 10.13422/j.cnki.syfjx.20191403.

摘要

目的：

研究老鹳草素对

-氨基半乳糖(

-GalN)致急性肝损伤小鼠的保护作用及机制。

方法：

60只小鼠随机分为正常组，模型组，水飞蓟素组(180 mg·kg

－1

)，老鹳草素低，中，高剂量组(50，100，200 mg·kg

－1

)，灌胃给药每天1次，正常组及模型组小鼠灌胃生理盐水连续10 d。末次给药2 h后，除正常组外，其余各组腹腔注射

-GalN(500 mg·kg

－1

)，建立

-GalN致小鼠急性肝损伤模型。16 h后，收集血清和肝组织。生化法测定血清中丙氨酸氨基转移酶(ALT)，天门冬氨酸氨基转移酶(AST)，碱性磷酸酶(ALP)，总胆红素(TBIL)和肝脏中丙二醛(MDA)，总超氧化物歧化酶(T-SOD)，谷胱甘肽过氧化物酶(GSH-Px)活性或含量；酶联免疫吸附测定(ELISA)检测血清中肿瘤坏死因子-

(TNF-

)，白细胞介素-1

(IL-1

)，白细胞介素-6(IL-6)和

-干扰素(IFN-

)含量；蛋白免疫印迹法(Western blot)分析肝组织中Toll样受体-4(TLR-4)和核转录因子-

B(NF-

B)蛋白表达情况；苏木素-伊红(HE)染色观察肝组织病理学变化。

结果：

与正常组比较，模型组小鼠血清中ALT，AST，ALP，TBIL和肝脏MDA水平显著升高(

0.01)，与模型组比较，老鹳草素各给药组能够明显降低肝损伤小鼠血清中ALT，AST，ALP，TBIL和肝脏MDA(

0.05，

0.01)，且增强肝脏T-SOD和GSH-Px活性(

0.05，

0.01)，同时抑制血清中TNF-

，IL-1

，IL-6和IFN-

含量的升高和TLR-4，NF-

B的蛋白表达(

0.05，

0.01)；HE染色结果显示其对肝组织损伤程度有明显改善作用。

结论：

综上所述，老鹳草素对

-GalN诱导的急性肝损伤小鼠具有显著的保护作用，其保肝作用机制可能与抑制氧化应激、炎症反应以及TLR-4/NF-

B信号通路有关。

Abstract

Objective:

To explore the hepatoprotective effect and its mechanism of the geraniin on mice with acute liver injury induced by

-galactosamine (

-GalN).

Method:

A total of 60 Kunming mice were randomly divided into normal group

model group

Silymarin group (positive group 180 mg·kg

-1

)

and low

medium and high-dose geraniin groups (50

100

200 mg·kg

-1

). All the mice were given with saline or corresponding dose of drugs (10 mL·kg

-1

) by gavage once a day for 10 d. After 2 h of the last administration

except the normal group

the mice of other groups were injected intraperitoneally with

-GalN (500 mg·kg

-1

) to induce the acute liver injury. After 16 h

the eye balls of mice were removed to take blood

and all mice were put to death to collect samples of liver. Activity or content of alanine aminotransferase (ALT)

aspartate aminotransferase (AST)

alkaline phosphatase (ALP)

total bilirubin (TBIL)

malondialdehyde (MDA)

total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) in liver were determined by biochemical method. The serum levels of tumor necrosis factor-

(TNF-

)

interleukin-1

(IL-1

)

-interferon (IFN-

) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA)

and expressions of Toll-like receptor-4(TLR-4) and nuclear factor(NF)-

B proteins were detected by Western blot. Liver histopathological changes were observed by hematoxylin-eosin (HE) staining.

Result:

Compared with the normal group

the serum levels of ALT

AST

ALP

TBIL and liver MDA in the model group were significantly increased (

0.05

0.01). Compared with the model group

geraniin can reduce activities or contents of ALT

AST

ALP

TBIL in serum and MDA in liver of mice (

0.05

0.01)

but increase activities of T-SOD and GSH-Px in liver (

0.05

0.01)

while inhibiting the contents of TNF-

IL-1

IL-6

IFN-

and the expressions of TLR-4 and NF-

B proteins in serum (

0.05

0.01). HE staining showed that acute liver injury of mice was alleviated obviously by geraniin.

Conclusion:

Geraniin has an obvious protective effective on acute liver injuries induced by

-GalN in mice. Its mechanism may be correlated with oxidative stress

inflammation and TLR-4/NF-

B signaling pathway.

关键词

Keywords

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