Analysis on Mechanism of Shema Zhichuan Liquid in Treating Asthma Based on Network Pharmacology

Xiu-fang HUANG; Yun-ji GAO; Gang LIAO; Hui-ting HUANG; Shao-feng ZHAN; Yan-ni LAI; Xiao-hong LIU

doi:10.13422/j.cnki.syfjx.20191461

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Analysis on Mechanism of Shema Zhichuan Liquid in Treating Asthma Based on Network Pharmacology

Data Mining | 更新时间：2021-02-09

- Analysis on Mechanism of Shema Zhichuan Liquid in Treating Asthma Based on Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 187-195(2019)
- 作者机构：
  
  1.广州中医药大学　第一临床医学院，广州　 510405；
  2.广州中医药大学　深圳医院，广东　深圳　 518000；
  3.广州中医药大学　第一附属医院　刘小虹广东省名中医传承工作室，广州　 510405
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191461
  CLC： R22; R256.12; R28; R318.04; R96
- Received：20 December 2018，
  
  Published Online：04 April 2019，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
Xiu-fang HUANG, Yun-ji GAO, Gang LIAO, et al. Analysis on Mechanism of Shema Zhichuan Liquid in Treating Asthma Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 187-195.
DOI：

Xiu-fang HUANG, Yun-ji GAO, Gang LIAO, et al. Analysis on Mechanism of Shema Zhichuan Liquid in Treating Asthma Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 187-195. DOI： 10.13422/j.cnki.syfjx.20191461.

摘要

目的：

运用网络药理学探讨射麻止喘液治疗哮喘的潜在机制。

方法：

利用中药分子机制的生物信息学分析工具、中药系统药理数据库和分析平台查找射麻止喘液的成分及其靶点，从比较毒理基因组学数据库(CTD)获取与哮喘相关的基因；将射麻止喘液-基因和哮喘-基因的数据集导入Draw Venn Diagram进行交集分析，得出射麻止喘液-哮喘-基因数据集；使用String 11.0进行射麻止喘液-哮喘-基因数据集的蛋白质-蛋白质相互作用(PPI)分析，运用Cytoscape 3.6.1进行可视化，用其插件MCODE分析重要模块；采用DAVID 6.8分析射麻止喘液-哮喘-基因的通路富集和生物学过程。

结果：

获得射麻止喘液399个化合物与2 099个潜在的靶点；同时检索出98个与哮喘相关靶点；应用交集和PPI分析筛选出45个共同基因和关键基因16个，包括转化生长因子-

，血红素氧合酶-1，白细胞介素-4等；富集分析显示射麻止喘液和哮喘的共同生物学过程与炎症反应、气道的收缩与重塑、细胞增殖与凋亡等相关，共同生物学通路包括肿瘤坏死因子信号通路、免疫球蛋白E(IgE)的高亲和力受体信号通路、核转录因子-

B信号通路、核苷酸结合寡聚化结构域样受体信号通路等。

结论：

射麻止喘液是通过多靶点、多通路治疗哮喘，可为其深入研究和临床应用提供参考。

Abstract

Objective:

To explore the potential mechanism of Shema Zhichuan liquid in treatment of asthma by network pharmacology.

Method:

Bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine (TCM)

systematic pharmacological database and analysis platform of TCM were employed to find the components in Shema Zhichuan liquid and their targets

and asthma-related genes were obtained from the comparative toxicogenomics database (CTD). The data set of Shema Zhichuan liquid-gene and asthma-gene were imported into the Draw Venn Diagram for intersection analysis. The obtained data set of Shema Zhichuan liquid-asthma-gene was imported into String 11.0 for protein-protein interaction (PPI) analysis

and was visualized by Cytoscape 3.6.1

and further important modules were analyzed with MCODE. DAVID 6.8 was used to analyze pathway enrichment and biological process of Shema Zhichuan liquid-asthma-gene.

Result:

A total of 399 components and 2 099 potential targets were obtained from Shema Zhichuan liquid

98 asthma-related targets were retrieved

45 common genes and 16 hub genes were screened

including transforming growth factor-

(TGF-

)

heme oxygenase-1 (HMOX1)

interleukin-4 (IL-4)

etc. Enrichment analysis showed that the common biological processes of Shema Zhichuan liquid and asthma were related to inflammation

contraction and remodeling of airway

cell proliferation and apoptosis

etc. The common biological pathways mainly included tumor necrosis factor (TNF) signaling pathway

receptor with high affinity for immunoglobulin E (Fc epsilon RI) signaling pathway

nuclear transcription factor-kappa B (NF-

B) signaling pathway

nucleotide binding oligomerization domain (NOD)-like receptor signaling pathway and so on.

Conclusion:

Shema Zhichuan liquid serves as a multi-target

multi-pathway treatment for asthma

which can provide a reference for the further research and clinical application of this preparation.

关键词

Keywords

references

Masoli M , Fabian D , Holt S , et al . The global burden of asthma: executive summary of the GINA Dissemination Committee report [J]. Allergy , 2004 , 59 ( 5 ): 469 - 478 .

HU X , WANG J , XIA Y , et al . Resveratrol induces cell cycle arrest and apoptosis in human eosinophils from asthm atic individuals [J]. Mol Med Rep , 2016 , 14 ( 6 ): 5231 - 5236 .

Hetherington K J , Heaney L G . Drug therapies in severe asthma-the era of stratified medicine [J]. Clin Med(Lond) , 2015 , 15 ( 5 ): 452 - 456 .

徐顺富，南淑玲，周涛．中医治疗哮喘临床进展 [J]．辽宁中医药大学学报， 2016 ， 18 ( 8 )： 150 - 153 ．

刘琼．射麻止喘液对支气管哮喘患者肺功能的影响 [J]．河北中医， 2005 ， 27 ( 10 )： 731 - 732 ．

陈芝喜，梁直英，周名璐，等．射麻止喘液对哮喘豚鼠血浆环核苷酸和TxB2、6-K-PGF1α水平的影响 [J]．放射免疫学杂志， 2000 ， 13 ( 2 )： 77 - 78 ．

钟亮环．射麻止喘液治疗哮喘发作期的临床疗效观察及其对外周血IL-5、IL-8的影响 [D]．广州：广州中医药大学， 2005 ．

周宇，刘建博，张玉芳，等．射麻止喘液对哮喘大鼠气道平滑肌细胞作用研究 [J]．辽宁中医药大学学报， 2011 ， 13 ( 1 )： 76 - 78 ．

汤化琪，梁瑞敏，陈宇，等． BATMAN-TCM在线分析工具预测玄胡索散治疗骨关节炎作用及初步验证研究 [J]．中华中医药学刊， 2018 ， 36 ( 1 )： 142 - 145 ．

赖艳妮，严一文，徐培平．基于系统药理学探索莪术有效成分的药理作用机制 [J]．中国实验方剂学杂志， 2017 ， 23 ( 14 )： 177 - 182 ．

LIANG B , LI C , ZHAO J . Identification of key pathways and genes in colorectal cancer using bioinformatics analysis [J]. Med Oncol , 2016 , 33 ( 10 ): 111 - 119 .

Ohta K , Nagase H , Suzukawa M , et al . Antibody therapy for the management of severe asthma with eosinophilic inflammation [J]. Int Immunol , 2017 , 29 ( 7 ): 337 - 343 .

Thomas B J , Kan O K , Loveland K L , et al . In the shadow of fibrosis: innate immune suppression mediated by transforming growth factor-beta [J]. Am J Respir Cell Mol Biol , 2016 , 55 ( 6 ): 759 - 766 .

LYU J , SU W , YU Q , et al . Heme oxygenase-1 protects airway epithelium against apoptosis by targeting the proinflammatory NLRP3-RXR axis in asthma [J]. J Biol Chem , 2018 , 293 ( 48 ): 18454 - 18465 .

Lewis T C , Metitiri E E , Mentz G B , et al . Influence of viral infection on the relationships between airway cytokines and lung function in asthmatic children [J]. Respir Res , 2018 , 19 ( 1 ): 228 - 241 .

WU W , LI Y , JIAO Z , et al . Phyllanthin and hypophyllanthin from Phyllanthus amarus ameliorates immune-inflammatory response in ovalbumin-induced asthma: role of IgE, Nrf2, iNOs, TNF-α, and IL's [J]. Immunopharmacol Immunotoxicol , 2018 , 19 ( 12 ): 1 - 13 .

LI Y , WANG H , YANG X . Effects of catalpol on bronchial asthma and its relationship with cytokines [J]. J Cell Biochem , 2018 , 12 ( 9 ): 1 - 7 .

XU M H , YUAN F L , WANG S J , et al . Association of interleukin-18 and asthma [J]. Inflammation , 2017 , 40 ( 1 ): 324 - 327 .

LV J , YU Q , LV J , et al . Airway epithelial TSLP production of TLR2 drives type 2 immunity in allergic airway inflammation [J]. Eur J Immunol , 2018 , 48 ( 11 ): 1838 - 1850 .

ZHANG Y , Willis-Owen S A G , Spiegel S , et al . The ORMDL3 asthma gene regulates ICAM1 and has multiple effects on cellular inflammation [J]. Am J Respir Crit Care Med , 2019 , 199 ( 4 ): 478 - 488 .

WU X L , LI R , ZHANG H W , et al . Methylation status of ORMDL3 regulates cytokine production and p-ERK/MMP9 pathway expression [J]. Exp Cell Res , 2018 , 372 ( 1 ): 43 - 51 .

Shin D , Park S H , Choi Y J , et al . Dietary compound kaempferol inhibits airway thickening induced by allergic reaction in a bovine serum albumin-induced model of asthma [J]. Int J Mol Sci , 2015 , 16 ( 12 ): 29980 - 29995 .

Pegorier S , Arouche N , Dombret M C , et al . Augmented epithelial endothelin-1 expression in refractory asthma [J]. J Allergy Clin Immunol , 2007 , 120 ( 6 ): 1301 - 1307 .

Curtis J L . Cell-mediated adaptive immune defense of the lungs [J]. Proc Am Thorac Soc , 2005 , 2 ( 5 ): 412 - 416 .

Takahashi H , Hashimoto Y , Ishida-Yamamoto A , et al . Roxithromycin suppresses involucrin expression by modul ation of activator protein-1 and nuclear factor-kappaB activities of keratinocytes [J]. J Dermatol Sci , 2005 , 39 ( 3 ): 175 - 182 .

Mishra V , Banga J , Silveyra P . Oxidative stress and cellular pathways of asthma and inflammation: Therapeutic strategies and pharmacological targets [J]. Pharmacol Ther , 2018 , 181 ( 8 ): 169 - 182 .

De Logu F , Patacchini R , Fontana G , et al . TRP functions in the broncho-pulmonary system [J]. Semin Iummunopathol , 2016 , 38 ( 3 ): 321 - 329 .

Arizmendi N , Kulka M . Adenosine activates Galphas proteins and inhibits C3a-induced activation of human mast cells [J]. Biochem Pharmacol , 2018 , 156 ( 8 ): 157 - 167 .

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