Effect of Modified Erchentang on PPARγ Expression in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease

Wen-ying XIE; Yong-sheng BAO; Jun-yue WANG; Li-zhi SHANG; Ke WU; Liang-zhi ZHANG; Liang LI; Xiao-yan WANG; Qian-hong SONG

doi:10.13422/j.cnki.syfjx.20191501

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Effect of Modified Erchentang on PPARγ Expression in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease

Study on the Effect of Modified Erchentang on Chronic Obstructive Pulmonary Disease | 更新时间：2021-02-09

- Effect of Modified Erchentang on PPARγ Expression in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 26-33(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191501
  CLC： R2-0; R22; R285.5; R289
- Received：30 December 2018，
  
  Published Online：18 April 2019，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
Wen-ying XIE, Yong-sheng BAO, Jun-yue WANG, et al. Effect of Modified Erchentang on PPARγ Expression in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 26-33.
DOI：

Wen-ying XIE, Yong-sheng BAO, Jun-yue WANG, et al. Effect of Modified Erchentang on PPARγ Expression in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 26-33. DOI： 10.13422/j.cnki.syfjx.20191501.

摘要

目的：

探索二陈汤加味对慢性阻塞性肺疾病(COPD)大鼠模型肺组织中过氧化物酶体增殖物激活受体

(PPAR

)蛋白和mRNA表达的影响，以及血清、肺组织匀浆液和支气管肺泡灌洗液中白细胞介素-6(IL-6)，白细胞介素-10(IL-10)，肿瘤坏死因子-

(TNF-

)表达的影响。

方法：

将70只SD大鼠随机分为7组，每组10只，分别为正常组、模型组、二陈汤加味高、中、低剂量(40，20，10 g·kg

－1

·d

－1

)组，消咳喘组(5 g·kg

－1

·d

－1

)，二陈汤组(5 g·kg

－1

·d

－1

)。以烟熏与脂多糖(LPS)气管滴注并用的方法来制备大鼠COPD模型。成功后，治疗组灌胃给药，正常及模型组则灌等量的生理盐水。酶联免疫吸附测定(ELISA)检测大鼠血清、肺组织匀浆液和支气管肺泡灌洗液(BALF)中IL-6，IL-10和TNF-

的含量。实时荧光定量聚合酶链式反应(Real-time PCR)检测PPAR

mRNA表达，免疫组化(IHC)，蛋白免疫印迹法(Western blot)检测肺组织PPAR

的蛋白表达。

结果：

与正常组比较，模型组大鼠血清、肺组织匀浆液和BALF中IL-6，TNF-

水平显著升高(

0.01)，IL-10水平显著降低(

0.01)。模型组大鼠肺组织PPAR

mRNA水平显著降低(

0.01)，蛋白表达也明显受抑制(

0.01)。与模型组比较，各治疗组血清、肺组织匀浆液及BALF中IL-6，TNF-

水平均不同程度的降低(

0.01)，IL-10则不同程度升高，其中二陈汤加味中剂量组IL-10的兴奋和IL-6，TNF-

的抑制尤为显著。各治疗组大鼠肺组织PPAR

mRNA和蛋白水平均有不同程度的升高(

0.01)。

结论：

二陈汤加味可能通过升高PPAR

的表达，并以此降低IL-6，TNF-

的含量，提升IL-10的含量，来对抗COPD大鼠的炎症反应，改善肺功能。

Abstract

Objective:

To explore the effect of modified Erchentang on peroxisome proliferator-activated receptor gamma (PPAR

) protein and gene expressions in lung tissue of chronic obstructive pulmonary disease (COPD) rat model

and the expressions of interleukin-6 (IL-6)

interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-

) in serum

lung homogenate and bronchoalveolar lavage fluid.

Method:

Seventy SD rats were randomly divided into seven groups: normal group

model group

high

medium and low-dose modified Erchentang groups (40

10 g·kg

-1

·d

-1

)

Xiaokechuan group (5 g·kg

-1

·d

-1

)

and Erchentang group (5 g·kg

-1

·d

-1

). The rat COPD model was established through smoking and intratracheal instillation of lipopolysaccharide (LPS). After successful modeling

the treatment group was given drug by gavage

while the normal group and the model group were given the same amount of saline. The concentrations of IL-6

IL-10 and TNF-

in serum

lung homogenate and bronchoalveolar lavage fluid(BALF) of rats were measured by enzyme-linked immunosorbent assay (ELISA). Real-time fluorescence quantitative PCR (Real-time PCR) was used to detect the expression of peroxisome proliferator-activated receptor gamma (PPAR

)

and immunohistochemistry (IHC) and Western blot were used to detect the expression of PPAR

in lung tissue.

Result:

Compared with the normal group

the levels of IL-6 and TNF-

in serum

lung homogenate and BALF of the model group rats increased significantly (

0.01)

while IL-10 decreased significantly (

0.01). The expressions of PPAR

mRNA in lung tissue of rats in model group were significantly decreased (

0.01)

and the expression of PPAR

protein was significantly inhibited(

0.01). Compared with the model group

the levels of IL-6 and TNF-

in serum

lung homogenate and BALF of each treatment group decreased to varying degrees(

0.01)

while IL-10 increased to varying degrees. The excitation of IL-10 and the inhibition of IL-6 and TNF-

in the middle-dose Erchentang group were particularly significant. The PPAR

mRNA and protein expressions in lung tissue of rats in each treatment group were increased to varying degrees (

0.01).

Conclusion:

Modified Erchentang may improve pulmonary inflammation and pulmonary function in COPD rats by increasing the expression of PPAR

and the content of IL-10 and decreasing the contents of IL-6 and TNF-

关键词

Keywords

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