Objective:

2

To investigate the effect of modified Ditantang on autophagy and relevant proteins in brain cells of rats with cerebral ischemia reperfusion injury.

Method:

2

The cerebral ischemic reperfusion (CIR) injury model in rats was built by reversible middle cerebral artery occlusion artery suture of middle cerebral embolism method

and randomly divided into sham group

model group

high-dose modified Ditantang group(H-dose)

low-dose modified Ditantang group (L-dose

0.384 g·kg

-1

) and PLXT group (0.1 g·kg

-1

). Sham and model groups were given normal saline by gastric perfusion

H-dose and L-dose groups were given modified Ditantang

and the PLXT group were given Piracetam tablets

intragastric volume 10 mL·kg

-1

. The treatment lasted for 7 d. Within 24 hours after administration

the histopathological examination was performed

the volume of cerebral infarction

neuronal apoptosis and serum levels of inflammatory factors were compared

and the expressions of autophagy related microtuble-associated protein 1 light chain 3(LC3)Ⅱ

Beclin1

B-cell lymphoma-2(Bcl-2) and p62 in brain tissues were determined.

Result:

2

Cells and blood vessel necrosis

neuron swelling and interstitial edema were observed in model group

a few neurons died

edema was reduced

swelling of nerve cells was alleviated in H-dose

L-dose and PLXT groups. The volume of cerebral infarction and neuronal apoptosis in H-dose

L-dose and PLXT groups were lower than those in model group (

P

<

0.05). The levels of tumor necrosis factor (TNF)-

α

intedeukin (IL)-2 and IL-8 in H-dose

L-dose and PLXT groups were lower than those in model group (

P

<

0.05). Compared with sham group

protein and mRNA expressions of LC3 Ⅱ

Bcl-2 and Beclin1 in the ischemic brain tissue of model group were significantly increased

while protein and mRNA expressions of p62 in the ischemic brain tissue of model group were decreased significantly. Compared with the model group

protein and mRNA expressions of LC3 Ⅱ and Beclin1 in H-dose

L-dose and PLXT groups were significantly decreased

while protein and mRNA expressions of p62 were significantly increased

with significant differences (

P

<

0.05).

Conclusion:

2

Modified Ditantang can improve brain injury and interfere with autophagy after MCAO/R

alleviate inflammation

and regulate autophagic activity

which may be related to the down-regulation of expressions of LC3 Ⅱ

Beclin1 and the up-regulation of expression of p62.